Transcript General Function of Rho-A

Role of Rho-A in
Glaucoma
Jasleen Kaur
Narmada Jayasankar
Vijetha Vemulapalli
Outline
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Open angle Glaucoma
RhoA pathway and hypothesis
CATPA
Open Angle Glaucoma
It can be defined as
damage to the Optic
nerve due to Increase in
Intra ocular pressure
(IOP)
Anatomy of the angle
Trabecular meshwork
Cornea
Retina
Shields textbook of glaucoma-2005
Cell cytoskeleton
Green: actin
Red: mitochondria
Blue: nucleus
TM Cell Contractility
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Aqueous humor regulation is done by TM cell
contractility and re-alignment of pores.
There are two pathways that regulate TM
contractility.
Ca dependent pathways
And the Ca independent pathway.
Rho-a protein is involved in the Ca
independent pathway.
General Function of Rho-A
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Involved in the maintenance of tight junctions.
Tight junctions are found in epithelial cells
and all cells where there is a separation or
demarcation into different regions.
If the Rho-A pathway does not function
properly in the eye, it causes Glaucoma.
Ca independent pathway
Relaxation
MLC
pMLC
Inactive MLCP
M20
Contraction
Active MLCP
PP1Cd
M20
MYPT1
PP1Cd
MYPT1
Plasma membrane
P
GAP
RhoA-GDP
RhoA-GTP
GEF
RhoA-GDP
GDI
GTP
GDP
Rho kinase
CATPA
CATPA
Point Mutations
Point Mutations
References
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1. http://biology.kenyon.edu/BMB/Chime2/
2002/rhoA/FRAMES/captxt.htm
2. http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=165390
3. PDB, CATH, SCOP
4. http://ajpcell.physiology.org/cgi/content/full/275/3/C798
5.http: //www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1220893
6. http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=14815
7. http://147.52.72.117/ijmm/2002/volume9/number6/563-570.pdf
8. http://apodaca2.dept-med.pitt.edu/PDFs/Rajasesekeran-TJs.pdf
9. www.ucl.ac.uk/ioo/research/greenwood.htm