Transcript Slide 1

Wang haitao
March 14 2011
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• Loss of one type of sensory input can cause improved
functionality of other sensory systems. Whereas this form
of plasticity, cross-modal plasticity, is well established, the
molecular and cellular mechanisms underlying it are still
unclear.
• blind individuals compensate their lack of visual input by
responding to somatosensory or auditory input with
improved sensitivity and accuracy. The brain can adapt to
sensory deprivation in one modality (e.g., visual cortex)
by increasing plasticity and retuning neuronal circuits in
other remaining modalities.
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Rectification index=
I (-60 mV)/ I(+40mV)
• GluR2-containing receptors
• GluR2-lacking receptors
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Methods
• In vitro whole cell recording
• Infection of Neocortical Neurons In Vivo
• In Vivo Microdialysis
• In Vivo Extracellular Recording
• Biochemical Analysis
• Immunohistochemistry
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VD Drives GluR1 into Layer 4 to Layer 2/3 Synapses
of the Barrel Cortex in Juvenile Rats
GFP-GluR1
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VD Drives GluR1 into Layer 4 to Layer 2/3 Synapses
of the Barrel Cortex in Juvenile Rats
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VD Procedure for 2 Days Occludes LTP
in the Barrel Cortex
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VD did not alter properties of NMDA component
and inhibitory component
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VD Procedure for 2 Days Occludes LTP in the
Barrel Cortex
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Serotonin Mediates VD-Driven Synaptic GluR1
Delivery in the Juvenile Rat Barrel Cortex
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Serotonin Mediates VD-Driven Synaptic GluR1 Delivery
in the Juvenile Rat Barrel Cortex
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Serotonin Facilitates Synaptic GluR1 Delivery
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Serotonin Facilitates Synaptic GluR1 Delivery
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Serotonin Facilitates Synaptic GluR1 Deliveryc
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VD Induces Phosphorylation of GluR1 at a Site
Critical for Its Synaptic Delivery
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VD Sharpens Functional Whisker-Barrel Map
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• Visual deprivation (VD) drives AMPA receptors
into synapses in the rat barrel cortex
• Seoronin mediates VD-induced facilitation of
synaptic AMPA receptor delivery
• VD increases activation of ERK and increase of
phosphorylation of GluR1 at Ser845
• VD sharpens the map in the barrel cortex
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Thanks
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