Transcript Histology Laboratories Molecules to Systems

Histology Laboratories
Molecules to Systems
2003
Compiled by
James D. Jamieson, MD/PhD
Thomas L. Lentz, MD
No part of this image collection may be distributed outside of the
Yale University Intranet.
Acknowledgements
Sources of Micrographs, Diagrams and Figures
Alberts, B. et al. Molecular Biology of the Cell. 4th Edition, Garland Science, New
York, 2002.
Gartner, L. P. and Hiatt, J. L. Color Atlas of Histology, Williams & Wilkins,
Baltimore, 1994.
Kerr, J. B. Atlas of Functional Histology. Mosby, London, 1999.
Kessel, R. G. and Kardon, R. H. Tissues and Organs: a text-atlas of scanning
electron microscopy. W. H. Freeman, San Francisco, 1979.
Lentz, T. L. Cell Fine Structure. W. B. Saunders, Philadelphia, 1971.
Lodish, H. et al. Molecular Cell Biology. W. H. Freeman, New York, 2000.
Mizoguti, H. Color Slide Atlas of Histology. Nihon Shashin Shinbunsha, Tokyo.
Young, B. and Heath, J. W. Wheater’s Functional Histology. Churchill
Livingstone, Edinburgh, 2000.
Micrographs taken by George Palade, Marilyn Farquhar, James D. Jamieson,
Nicolai Simionescu, Maya Simionescu, David Castle, Thomas L. Lentz.
Web Resources
http://info.med.yale.edu/webpath/webpath.htm
Cushing Library Educational Software/Cell Biology/Several Histology Resources
Nervous System Laboratory
Spinal Cord
GM
Dorsal root ganglia
WM
V root
Spinal Cord; Transverse Section
Motor Neurons
Ventral Horn
Dendrite
Nissl substance = RER
Axon hillock
Sensory Ganglia
Dorsal Root
Peripheral Nerves
Endoneurium
Perineurium
Epineurium
Myelinated Nerve: XS
Epineurium
Unmyelinated Nerve: XS
Axons
Schwann cell nuclei
Peripheral Nerve: LS
NR
Axon
Myelin Sheath: Node of Ranvier
Myelin
A
A
Schwann cell cyto.
Unmyelinated Axon; LS
Schwann cell nucleus
A
A
Myelinated Axons
Cerebral Cortex
Cerebral cortex
Cerebellum
Horizontal Section
GM
WM
Cerebral Cortex
Nerve cell body
Axons
Glial cells
Grey Matter
Astrocytes
Neuroglia
Cerebellum
Molecular layer
Purkinje cell layer
Granular layer
Cerebellum
Cerebellum: Purkinje Cells
ML
Dendrites
PC
GL
Some pathology to think about.
Alzheimer’s Brain: Gross
Alzheimer’s Brain, H&E
Do you see anything different in the cytoplasm of the neurons? What might this do to them?
Normal Brain Compared to Brain from Parkinson’s Patient, H&E
Which section is from the normal brain and why do you conclude this?
Brain From Stroke Patient
Section from Brain of Stroke victim
The results of anoxia. Which cells are affected?
Brain from Infant with Tay-Sach’s Disease
What organelles in these cells are defective and how does the morphology reflect this?
Cerebrum from Patient with Creutzfeldt-Jacob Disease.
Note “holes” in cortex resulting from prion amyloid deposits. Human form of “mad cow disease: bovine spongioform encephalopathy”.