Transcript Lecture 4

Chapter 4
Functional Anatomy of Prokaryotic
Cells
part A
Comparing Prokaryotic and Eukaryotic Cells
• All cells, whether they are prokaryotic or eukaryotic, have some
common features.
– Plasma membrane - a phospholipid bilayer with proteins
• separates the cell from the surrounding environment
• functions as a selective barrier for the import and export of materials
– Cytoplasm - The rest of the material of the cell within the plasma membrane
• excluding the nucleoid region or nucleus
• consists of a fluid portion called the cytosol and the organelles and other
particulates suspended in it
– DNA
• The genetic material contained in one or more chromosomes
– Ribosomes
• the organelles on which protein synthesis takes place
Comparing Prokaryotic and Eukaryotic Cells
Prokaryote (no nucleolus)
• One circular chromosome, not
in a membrane
• No histones
• Peptidoglycan cell walls
• No organelles
• Binary fission
Eukaryote (true nucleolus)
- Paired chromosomes, in
nuclear membrane
-Histones
- Polysaccharide cell walls
- Organelles
- Mitotic spindle
Size and shape of bacterial cells
• Average size: 0.2 -1.0 µm  2 - 8 µm
• Basic shapes:
– spherical (coccus),
– rod-shaped (bacillus, coccobacillus)
– Spiral (vibrio, spirillum, spirochete)
• Unusual shapes
– Star-shaped Stella
– Square Haloarcula
• Most bacteria are monomorphic
• A few are pleomorphic
Arrangements
• Depend on the planes of dividing bacteria form different structures
Plane of
division
Diplococci
Single bacillus
Streptococci
Diplobacilli
Tetrad
Streptobacilli
Sarcinae
Coccobacillus
Staphylococci
.
Typical structure of a prokaryoticCellcell
wall
Capsule
Pilus
The drawing below and the micrograph at right
show a bacterium sectioned lengthwise to reveal
the internal composition. Not all bacteria have all
the structures shown; only structures labeled in
red are found in all bacteria.
Cytoplasm
70S Ribosomes
Plasma membrane
Nucleoid containing DNA
Inclusions
Plasmid
Fimbriae
Capsule
Cell wall
Plasma
membrane
Flagella
.
© 2013 Pearson Education, Inc.
Although the nucleoid appears split in
the photomicrograph, the thinness of
the “slice” does not convey the
object’s depth.
Cell Wall
•
They are composed of unique components found nowhere else in
nature.
– Bacteria - Peptidoglycan
• Exceptions:
– Mycoplasmas – Lack cell walls
– Archaea – no peptidoglycan
• The cell wall of bacteria is an essential structure for viability.
– Protects the cell (protoplast )from mechanical damage and from osmotic
rupture or lysis
• They provide ligands for adherence.
• They cause symptoms of disease in animals.
• Receptor sites for drugs or viruses.
– They are one of the most important sites for attack by antibiotics.
• They provide immunological variation among strains of bacteria.
Figure 4.6a, b
Cell Wall - Peptidoglycan
• Polymer of disaccharide
(NAG-NAM)n
N-acetylglucosamine (NAG) - N-acetylmuramic acid (NAM)
• Linked by polypeptides
Figure 4.13a
Cell Wall - Structure
• Almost all bacteria can be divided into two large groups based on
the levels of peptidoglycan and physical properties of their cell
walls.
– Gram positive
– Gram negative
Gram-positive
Gram-negative
Gram-Positive cell walls
• The cell wall is thick (15-80 nanometers), consisting of several
layers of peptidoglycan.
• Running perpendicular to the peptidoglycan sheets are a group of
molecules called teichoic acids which are unique to the Grampositive cell wall.
– Wall teichoic acid links to peptidoglycan
– Lipoteichoic acid links to plasma membrane
Figure 4.13b
Gram-Negative cell walls
• The cell wall is relatively thin (10 nanometers) and is composed of:
1. A single layer of peptidoglycan
2. Outer membrane - part of the cell wall.
• Outer membrane composition is distinct from that of the cytoplasmic
membrane
• Unique component, lipopolysaccharide (LPS or endotoxin), which is
toxic to animals.
– O polysaccharide part - antigen
– Lipid A - endotoxin
• Porins (proteins) form channels through
membrane
• Protection from phagocytes, complement,
antibiotics.
3. Periplasm – area between the outer membrane and the plasma membrane.
Cell wall
Gram-positive cell walls
• Thick peptidoglycan
• Teichoic acids
• In acid-fast cells, contains
mycolic acid
Gram-negative cell walls
Thin peptidoglycan
No teichoic acids
Outer membrane
Lipid A
Atypical Cell Walls
• Mycoplasmas
– Lack cell walls
– Have sterol-like molecules incorporated into their membranes and they are
usually inhabitants of osmotically-protected environments (contain a high
concentration of external solute)
• Archaea
– Walls of pseudomurein (lack NAM and D amino acids)
– Wall-less
Damage to Cell Walls
• Lysozyme digests disaccharide in peptidoglycan.
– Protoplast is a wall-less cell.
– Spheroplast is a bacterial cell with a cell wall that has been
altered or is partly missing, resulting in a spherical shape.
– L forms are wall-less cells that swell into irregular shapes.
– Protoplasts and spheroplasts are susceptible to osmotic lysis.
• Penicillin (beta-lactam antibiotics) inhibits peptide bridges in
peptidoglycan.
Glycocalix
• Glycocalix means sugar coat
• External to the cell (outside of cell wall)
– Many prokaryotes secrete a substance on their surface.
– Usually sticky – polysaccharide, polypeptide or both.
• If the substance is organized and is firmly attached to the cell
wall - capsule
• If the substance is unorganized and loosely attached to the cell
wall –slime layer
• Function
– Extracellular polysaccharide
allows cell to attach
– Capsules prevent phagocytosis
Figure 4.6a, b
Flagella
• Some prokaryotic cells have a flagellum or flagella (plural) tail-like structure that projects from the cell body
– Outside cell wall
– Different arrangement
• Made of chains of protein flagellin
• Attached to a protein hook
• Anchored to the wall and membrane by the basal body
– 2 or 4 rings
Figure 4.8
Flagella
• Function - locomotion
– Rotate flagella to run, swim or tumble
– Some bacteria can “swarm”
• Move toward or away from particular stimuli (taxis) –
chemotaxis and phototaxis
• Flagella proteins are H antigens –useful for distinguishing
among variation within the species
Axial Filaments
• Endoflagella
• Structure similar to flagella
• Located in the periplasmic space (between the cell membrane
and outer membrane) , anchored at one end of a cell
• Rotation causes cell to move
• In Spirochaetes
Figure 4.10a
Fimbriae and pili
• Fimbriae - hair like appendages that are
shorter, straighter and thinner then
flagella
– Used for attachment
• Pili are usually longer than fimbriae,
only one or two per cell
– Used to transfer DNA from one cell
to another - conjugation
Plasma Membrane
• Phospholipid bilayer
Plasma Membrane
• Transmembrane proteins
– Peripheral proteins – easily removed from the membrane
– Integral proteins- can be removed only after disrupting
the lipid bilayer
• Some integral protein are channels
Figure 4.14b
Fluid Mosaic Model
•
•
•
•
Membrane is as viscous as olive oil.
Proteins move to function
Phospholipids rotate and move laterally
The dynamic arrangement of phospholipids and proteins
is referred to as the fluid mosaic model
Figure 4.14b
Functions of the prokaryotic plasma membrane
•
•
Osmotic or permeability barrier.
Location of transport systems for specific solutes (nutrients and
ions).
• Energy generating functions
– Involving respiratory and photosynthetic electron transport systems
• establishment of proton motive force, and ATP-synthesizing ATPase
• Synthesis of membrane lipids
• including lipopolysaccharide in Gram-negative cells
• Synthesis of murein (cell wall peptidoglycan)
• Coordination of DNA replication and segregation with septum
formation and cell division
• Location of specialized enzyme systems
– CO2 fixation - Photosynthetic pigments
– nitrogen fixation
Figure 4.15
Movement Across Membrane
• Selective permeability allows passage of some molecules
• Materials move across plasma membrane of both prokaryotic and eukaryotic
cells by two kings of process:
• Passive process
•
Simple diffusion: Movement of molecules or ions from an area of high concentration to
an area of low concentration.
• Osmosis: Movement of water (solvent molecules) across a selectively permeable
membrane from an area of high water concentration to an area of lower water.
• Facilitated diffusion is a carrier-mediated system that does not require energy and does
not concentrate solutes against a gradient
• Active process
•
Active transport – move the substances across the plasma membrane,
usually from outside to inside against concentration gradient
•
•
Cell uses a transporter protein and energy in the form of ATP
Group translocation of substances – the substance is chemically altered
during the transport (used by bacteria for sugar uptake) .
• Energy from phosphoenolpyruvic acid ( PEP), typical for bacteria
Movement Across Membrane
Plasma Membrane
• Damage to the membrane:
– Alcohols
– Quaternary ammonium salts (detergents) and
– Polymyxin antibiotics causes leakage of cell contents.
Cytoplasm
• Cytoplasm is the substance inside the plasma membrane
– Consist of about 80% water
– Contains primary proteins (enzymes),carbohydrates, lipids, inorganic
ions and many low-molecular weight compounds
– Protein filaments most likely responsible for the rod and helical cell
shapes of bacteria
Figure 4.6a, b
Figure 4.6 - Overview
Nuclear Area
• Nuclear area - Nucleoid - bacterial chromosome
– Long, continuous, circularly arranged double stranded DNA
• Not surrounded of nuclear membrane
• The chromosome is attached to the plasma membrane
• In actively growing bacteria, as much as 20% of the cell volume is
occupied by DNA.
Figure 4.6a, b
Plasmids
• Small circular, double-stranded DNA molecules.
• Extrachromosomal genetic elements
• They replicate independently of chromosomal DNA
– The cell can carry from one to hundreds of copies of a plasmid
• Contain 5 – 100 genes, generally not crucial for the survival of
bacteria under normal environmental condition.
– Plasmids may carry genes for such activities as antibiotic resistance,
tolerance to toxic metals, the production of toxins, and the synthesis of
enzymes.
• Plasmids can be transferred from one bacteria to another.
Ribosomes
• Ribosomes are cellular structures which function as the
sites of protein synthesis
• Prokaryotic cell contains tens of thousands of this small
structures, which give the cytoplasm a granular appearance
Figure 4.6 - Overview
Ribosomes
• Ribosomes are composed of two subunits – small and large
• The subunits consists of:
– Very large RNA molecules (known as ribosomal RNA or rRNA)
– Multiple smaller protein molecules
• Prokaryotic and Eukaryotic ribosomes differ in the number of proteins and rRNA
molecules they contain.
• Prokaryotic ribosomes are called 70S ribosomes
- A small unit 30S - one molecule of RNA - rRNA
- A larger 50S subunit – two molecules of RNA
S - The sedimentation coefficient of a particle is used to characterize its behaviour in sedimentation processes,
notably centrifugation
Figure 4.19
Ribosomes
• Because of differences in prokaryotic and eukaryotic ribosomes,
the microbial cell can be killed by antibiotic while the eukaryotic
host cell remains unaffected.
• Several antibiotics work by inhibiting protein synthesis on
prokaryotic cells.
– Streptomycin and Gentamycin – attach to the 30S subunits
– Erythromycin and chloramphenicol - attach to the 50S subunits
Reserve deposits - Inclusions
• Metachromatic granules
(volutin)
• Polysaccharide granules
• Lipid inclusions
• Sulfur granules
• Carboxysomes
•Phosphate reserves
• Gas vacuoles
•Protein covered cylinders
•Iron oxide
(destroys H2O2)
• Magnetosomes
•Energy reserves
•Energy reserves
•Energy reserves
•Ribulose 1,5-diphosphate
carboxylase for CO2 fixation
Endospores
• When essential nutrients are depleted, certain gram-positive bacteria
form specialized “resting” cells called endospores
– The primary function of most endospores is to ensure the survival of a
bacterium through periods of environmental stress
– Sporulation in bacteria is not
a mean of reproduction
• Unique to bacteria
– Durable dehydrated cells with thick walls and additional layers
– Resistant to desiccation, heat, chemicals
– They are formed internal to the bacterial cell membrane
• Located- terminally, sub terminally or centrally
• Endospores can remain dormant for thousands of years
• Germination: Return to vegetative state
– Germination is triggered by physical or chemical damage to the endospore’s
coat
Endospores
• Sporulation - endospore formation
– Spore septum
– Forespore - a structure entirely enclosed
within the original cell
– Peptidoglycane layers
– Spore coat
– Release
• Endospore core contains
–
–
–
–
–
DNA,
Small amounts of RNA,
Ribosomes,
Enzymes
Few important small molecules.
Cell division –Binary fission
Learning objectives
• Compare and contrast the overall cell structure of prokaryotes and
eukaryotes.
• Identify the three basic shapes of bacteria
• Describe the structure and function of the clycocalyx, flagella, axial
filaments, fimbriae, and pili.
• Compare and contrast the cell walls of gram-positive bacteria, gram
negative bacteria, acid-fast bacteria, archaea, and mycoplasmas.
• Describe the structure, chemistry and function of the prokaryotic
plasma membrane
• Define simple diffusion, osmosis, active transport, and group
translocation
• Identify the function of the nuclear area, ribosomes, and inclusions
• Describe the functions of endospores, - sporulation, and endospore
germination.
• Describe the process of binary fission