Transcript Structure cellulaire

Cellular Structure
Does there exist a unit of living on
the cellular structure?
working hypothesis
• Does there are a unit of living on the cellular structure?
- For test this:
- Study of eucaryot (animal cell, vegetable cell and
yeast)
- Study of procaryot (bacteria)
- Does there are a unit of living on the cellular chimical
composition
- Research of chimical composition by tests to
determine if there are presence of glucid? lipid?
and protein?
Does there are a unit of
living on the cellular
structure?
Observation without microscope
• Only birds eggs are visible cell without microscope,
we can seen a membrane, a nucleus to join egg
yolk.
the colorants to
see invisible
• The blue of méthylène enable to bring
out oxydoreduction reaction.
• The red Soudan III enable to bring out
lipids.
• Liquor of Fehling enable to bring out
glucids.
• The reagent of Bradford enable to
bring out protids (proteins).
Vegetable cell
• Oignon cell color to blue of méthylène observe at the optical
microscope.
squeleton wall
nucleus
cytoplasme
X 400
Animal cell
• We have take oral cell colored with blue of
méthylène observed the optical microscope.
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membrane
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nucleus
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cytoplasm
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X400
yeast
• We have take yeast cell to underline thanks to blue of
méthylène. The yeast is a animal organism unicellular.
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X400
Bacteria and yeast
yeast
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cytoplasm
nucleus
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membrane
bactérie
taking to stem of the garden pool inside
d’Hippocampe
X1000
Bacteria
• We have observed bacteria with blue of
méthylène
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bacterium
X1000
is there unit living on a level
with chimical composition of cell
Glucides
• Fehling liquor we have allow to detect presence of
glucides in the cells studied (banana ,onion ,eggs
,…).
Lipids
• Lipids react with Red Soudan III .
Or at the microscope the membranes appear red so
we can say that : they contain lipids. In fact the
cells are surround of a plasma membrane ,which is
a fundamental barrier between cytoplasm and
milieu outside of cell. The membrane is a safty film
very thin which contain protein and lipids.
• According to our experiences all cells contiain
lipids.
Protein
• To briging to ligth proteins we have used
Bradford’s reactive in solution with
differents cells. this reactive colored
proteins allow for their concentration in the
solution. Next, we introduce this solution
in a spectrophotometer which we give the
absorbation of the solution. More the
absorbation is exalted more the
concentration in protein is pronounced.
• All cells have got proteins according to
our experiences
Protein migration
• Does there are all cell have got the same
protein?
•
Test of migration protein
1.Rule : gel electrophoresis allow to
make migrate proteins allow for their
molecular weight
2.Schema of gel
1.Evidence test
2.Bacteria
3.yeast
4.Animal cell
5. Végétal cell
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the gel after protein migration
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1.Evidence test 2.Bacteria 3.yeast 4.Animal cell 5.Végétal cell
Results
• The eucaryots and the procaryots have
different height and différent form. However
they have similarity like the membrane the
genetic material and the cytoplasm.
• We can conclued according to our
experiences than all cells have similarity
because they all contain lipids,proteins and
glucids. Howevere we don’t know exactly
what they compil which contain the same
lipids, proteins and glucids.