Transcript 12. Cell Cycle, Mitosis

Chapter 12
The Cell Cycle
PowerPoint Lectures for
Biology, Seventh Edition
Neil Campbell and Jane Reece
Lectures by Chris Romero
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Overview: The Key Roles of Cell Division
• The ability of organisms to reproduce best
distinguishes living things from nonliving matter
• The continuity of life is based upon the
reproduction of cells, or cell division
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
• In unicellular organisms, division of one cell
reproduces the entire organism
• Multicellular organisms depend on cell division for:
– Development from a fertilized cell
– Growth
– Repair
• Cell division is an integral part of the cell cycle, the
life of a cell from formation to its own division
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 12-2
100 µm
Reproduction
200 µm
Growth and development
20 µm
Tissue renewal
Concept 12.1: Cell division results in genetically
identical daughter cells
• Cells duplicate their genetic material before they
divide, ensuring that each daughter cell receives
an exact copy of the genetic material, DNA
• A dividing cell duplicates its DNA, allocates the
two copies to opposite ends of the cell, and only
then splits into daughter cells
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Cellular Organization of the Genetic Material
• A cell’s endowment of DNA (its genetic
information) is called its genome
• DNA molecules in a cell are packaged into
chromosomes
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
• Every eukaryotic species has a characteristic
number of chromosomes in each cell nucleus
• Somatic (nonreproductive) cells have two sets of
chromosomes
• Gametes (reproductive cells: sperm and eggs)
have half as many chromosomes as somatic cells
• Eukaryotic chromosomes consist of chromatin, a
complex of DNA and protein that condenses
during cell division
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 12-3
25 µm
Distribution of Chromosomes During Cell Division
• In preparation for cell division, DNA is replicated
and the chromosomes condense
• Each duplicated chromosome has two sister
chromatids, which separate during cell division
• The centromere is the narrow “waist” of the
duplicated chromosome, where the two
chromatids are most closely attached
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 12-4
0.5 µm
Chromosome
duplication
(including DNA
synthesis)
Centromere
Sister
chromatids
Separation
of sister
chromatids
Centromeres
Sister chromatids
• Eukaryotic cell division consists of:
– Mitosis, the division of the nucleus
– Cytokinesis, the division of the cytoplasm
• Gametes are produced by a variation of cell
division called meiosis
• Meiosis yields nonidentical daughter cells that
have only one set of chromosomes, half as many
as the parent cell
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Concept 12.2: The mitotic phase alternates with
interphase in the cell cycle
• In 1882, the German anatomist Walther Flemming
developed dyes to observe chromosomes during
mitosis and cytokinesis
• To Flemming, it appeared that the cell simply grew
larger between one cell division and the next
• Now we know that many critical events occur
during this stage in a cell’s life
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Phases of the Cell Cycle
• The cell cycle consists of
– Mitotic (M) phase (mitosis and cytokinesis)
– Interphase (cell growth and copying of
chromosomes in preparation for cell division)
• Interphase (about 90% of the cell cycle) can be
divided into subphases:
– G1 phase (“first gap”)
– S phase (“synthesis”)
– G2 phase (“second gap”)
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 12-5
INTERPHASE
G1
S
(DNA synthesis)
G2
• Mitosis is conventionally divided into five phases:
– Prophase
– Prometaphase
– Metaphase
– Anaphase
– Telophase
• Cytokinesis is well underway by late telophase
[Animations and videos listed on slide following figure]
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Fig. 12.9
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 12-6ca
G2 OF INTERPHASE
PROPHASE
PROMETAPHASE
LE 12-6da
METAPHASE
ANAPHASE
TELOPHASE AND CYTOKINESIS
Video: Animal Mitosis
Video: Sea Urchin (time lapse)
Animation: Mitosis (All Phases)
Animation: Mitosis Overview
Animation: Late Interphase
Animation: Prophase
Animation: Prometaphase
Animation: Metaphase
Animation: Anaphase
Animation: Telophase
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Check these web sites:
• http://www.cellsalive.com
• http:/www.biology.arizona.edu/cell_bio/tutorials/cell_cycle/cell
s2.html
•
http://www.biology.arizona.edu/cell_bio/cell_bio.html
• http://www.loci.wisc.edu/outreach/bioclips/CDBio.html
• http://gslc.genetics.utah.edu/units/stemcells/whatissc
Play this game:
• http://science.nhmccd.edu/biol/bio1int.htm
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
The Mitotic Spindle: A Closer Look
• The mitotic spindle is an apparatus of
microtubules that controls chromosome movement
during mitosis
• Assembly of spindle microtubules begins in the
centrosome, the microtubule organizing center
• The centrosome replicates, forming two
centrosomes that migrate to opposite ends of the
cell, as spindle microtubules grow out from them
• An aster (a radial array of short microtubules)
extends from each centrosome
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
• The spindle includes the centrosomes, the spindle
microtubules, and the asters
• Some spindle microtubules attach to the
kinetochores of chromosomes and move the
chromosomes to the metaphase plate
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 12-7
Aster
Microtubules
Sister
chromatids
Chromosomes
Centrosome
Metaphase
plate
Kinetochores
Overlapping
nonkinetochore
microtubules
Centrosome
1 µm
Kinetochore
microtubules
0.5 µm
• In anaphase, sister chromatids separate and
move along the kinetochore microtubules toward
opposite ends of the cell
• The microtubules shorten by depolymerizing at
their kinetochore ends
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 12-8b
Chromosome
movement
Microtubule
Motor
protein
Chromosome
Kinetochore
Tubulin
subunits
• Nonkinetochore microtubules from opposite poles
overlap and push against each other, elongating
the cell
• In telophase, genetically identical daughter nuclei
form at opposite ends of the cell
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Cytokinesis: A Closer Look
• In animal cells, cytokinesis occurs by a process
known as cleavage, forming a cleavage furrow
• In plant cells, a cell plate forms during cytokinesis
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 12-9a
100 µm
Cleavage furrow
Contractile ring of
microfilaments
Daughter cells
Cleavage of an animal cell (SEM)
LE 12-9b
Vesicles
forming
cell plate
Wall of
parent cell
Cell plate
1 µm
New cell wall
Daughter cells
Cell plate formation in a plant cell (TEM)
LE 12-10
Nucleus
Nucleolus
Chromatin
condensing
Prophase. The
chromatin is condensing.
The nucleolus is
beginning to disappear.
Although not yet visible
in the micrograph, the
mitotic spindle is starting
to form.
Chromosomes
Prometaphase. We
now see discrete
chromosomes; each
consists of two identical
sister chromatids. Later
in prometaphase, the
nuclear envelope will
fragment.
Cell plate
Metaphase. The spindle is
complete, and the
chromosomes, attached
to microtubules at their
kinetochores, are all at
the metaphase plate.
Anaphase. The
chromatids of each
chromosome have
separated, and the
daughter chromosomes
are moving to the ends of
the cell as their
kinetochore microtubules shorten.
10 µm
Telophase. Daughter
nuclei are forming.
Meanwhile, cytokinesis
has started: The cell
plate, which will divide
the cytoplasm in two, is
growing toward the
perimeter of the parent
cell.
Binary Fission
• Prokaryotes (bacteria and archaea) reproduce by
a type of cell division called binary fission
• In binary fission, the chromosome replicates
(beginning at the origin of replication), and the two
daughter chromosomes actively move apart
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 12-11_1
Cell wall
Origin of
replication
Plasma
membrane
E. coli cell
Chromosome
replication begins.
Soon thereafter,
one copy of the origin
moves rapidly toward
the other end of the cell.
Two copies
of origin
Bacterial
chromosome
LE 12-11_2
Cell wall
Origin of
replication
Plasma
membrane
E. coli cell
Chromosome
replication begins.
Soon thereafter,
one copy of the origin
moves rapidly toward
the other end of the cell.
Replication continues.
One copy of the origin
is now at each end of
the cell.
Bacterial
chromosome
Two copies
of origin
Origin
Origin
LE 12-11_3
Cell wall
Origin of
replication
E. coli cell
Chromosome
replication begins.
Soon thereafter,
one copy of the origin
moves rapidly toward
the other end of the cell.
Replication continues.
One copy of the origin
is now at each end of
the cell.
Replication finishes.
The plasma membrane
grows inward, and
new cell wall is
deposited.
Two daughter
cells result.
Plasma
membrane
Bacterial
chromosome
Two copies
of origin
Origin
Origin
The Evolution of Mitosis
• Since prokaryotes evolved before eukaryotes,
mitosis probably evolved from binary fission
• Certain protists exhibit types of cell division that
seem intermediate between binary fission and
mitosis
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 12-12
Bacterial
chromosome
Prokaryotes
Chromosomes
Microtubules
Intact nuclear
envelope
Dinoflagellates
Kinetochore
microtubules
Intact nuclear
envelope
Diatoms
Kinetochore
microtubules
Centrosome
Fragments of
nuclear envelope
Most eukaryotes
Concept 12.3: The cell cycle is regulated by a
molecular control system
• The frequency of cell division varies with the type
of cell
• These cell cycle differences result from regulation
at the molecular level
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Evidence for Cytoplasmic Signals
• The cell cycle appears to be driven by specific
chemical signals present in the cytoplasm
• Some evidence for this hypothesis comes from
experiments in which cultured mammalian cells at
different phases of the cell cycle were fused to
form a single cell with two nuclei
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 12-13
Experiment 1
Experiment 2
S
G1
M
G1
S
S
M
M
When a cell in the S phase was
fused with a cell in G1, the G1
cell immediately entered the
S phase—DNA was synthesized.
When a cell in the M phase
was fused with a cell in G1,
the G1 cell immediately began
mitosis—a spindle formed and
chromatin condensed, even
though the chromosome had
not been duplicated.
The Cell Cycle Control System
• The sequential events of the cell cycle are
directed by a distinct cell cycle control system,
which is similar to a clock
• The clock has specific checkpoints where the cell
cycle stops until a go-ahead signal is received
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 12-14
G1 checkpoint
Control
system
G1
M
M checkpoint
G2 checkpoint
G2
S
• For many cells, the G1 checkpoint seems to be the
most important one
• If a cell receives a go-ahead signal at the G1
checkpoint, it will usually complete the S, G2, and
M phases and divide
• If the cell does not receive the go-ahead signal, it
will exit the cycle, switching into a nondividing
state called the G0 phase
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 12-15
G0
G1 checkpoint
G1
If a cell receives a go-ahead
signal at the G1 checkpoint,
the cell continues on in the
cell cycle.
G1
If a cell does not receive a
go-ahead signal at the G1
checkpoint, the cell exits the
cell cycle and goes into G0, a
nondividing state.
The Cell Cycle Clock: Cyclins and
Cyclin-Dependent Kinases
• Two types of regulatory proteins are involved in
cell cycle control: cyclins and cyclin-dependent
kinases (Cdks)
• The activity of cyclins and Cdks fluctuates
during the cell cycle
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 12-16a
M
G1
S
G2
M
G1
S
G2
M
MPF activity
Cyclin
Time
Fluctuation of MPF activity and cyclin concentration
during the cell cycle
LE 12-16b
Cdk
Degraded
cyclin
G2
Cdk
checkpoint
Cyclin is
degraded
MPF
Cyclin
Molecular mechanisms that help regulate the cell cycle
Stop and Go Signs: Internal and External Signals at
the Checkpoints
• An example of an internal signal is that
kinetochores not attached to spindle
microtubules send a molecular signal that
delays anaphase
• Some external signals are growth factors,
proteins released by certain cells that stimulate
other cells to divide
• For example, platelet-derived growth factor
(PDGF) stimulates the division of human
fibroblast cells in culture
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
• A variety of chemical and physical factors can influence cell
division.
Important for mammalian cells are growth factors, proteins
released by one group of cells that stimulate other cells to divide.
• Each cell type responds specifically to a certain growth factor or
combination of factors.
– For example, platelet-derived growth factors
(PDGF), produced by platelet blood cells, bind to
tyrosine-kinase receptors of fibroblasts, a type of
connective tissue cell.
– This triggers a signal-transduction pathway that
leads to cell division.
Copyright
© 2005©Pearson
Education,Education,
Inc. publishing
as Benjamin
Cummings
Copyright
2002 Pearson
Inc.,
publishing
as Benjamin
Cummings
LE 12-17
Scalpels
Petri
plate
Without PDGF
With PDGF
Without PDGF
With PDGF
10 mm
• Another example of external signals is densitydependent inhibition, in which crowded cells
stop dividing
• Most animal cells also exhibit anchorage
dependence, in which they must be attached to a
substratum in order to divide
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 12-18a
Cells anchor to dish surface and
divide (anchorage dependence).
When cells have formed a complete
single layer, they stop dividing
(density-dependent inhibition).
If some cells are scraped away, the
remaining cells divide to fill the gap and
then stop (density-dependent inhibition).
Normal mammalian cells
25 µm
Regulation of the Cell Cycle
• How cell division is regulated is very complex and
when there are errors it results in cancer
• Cancer is a disease where control of cell
division is lost. The cells don’t grow or behave
normally
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
• Cancer cells exhibit neither density-dependent
inhibition nor anchorage dependence
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 12-18b
Cancer cells do not exhibit
anchorage dependence
or density-dependent inhibition.
25 µm
Cancer cells
Loss of Cell Cycle Controls in Cancer Cells
• Cancer cells do not respond normally to the body’s
control mechanisms
• Cancer cells form tumors, masses of abnormal
cells within otherwise normal tissue
• If abnormal cells remain at the original site, the
lump is called a benign tumor
• Malignant tumors invade surrounding tissues and
can metastasize, exporting cancer cells to other
parts of the body, where they may form secondary
tumors
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
tumors
Growing out of control, cancer cells produces
malignant tumors
– Cancer cells
• divide excessively to form masses called
tumors
Tumors can be benign and malignant
(cancerous neoplasm)
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
What is a metastasis?
1.Cancer cells break away from tumor
2. Cancer cells migrate to blood vessels. These cells are able
to secrete enzymes that open vessel wall and enter the
blood stream or lymph.
3. Cells move with the blood, exit vessel and invade a new
site.
4. This new growth on a new site is a metastasis
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 12-19
Lymph
vessel
Tumor
Blood
vessel
Glandular
tissue
Cancer cell
A tumor grows from a
single cancer cell.
Cancer cells invade
neighboring tissue.
Cancer cells spread
through lymph and
blood vessels to
other parts of the
body.
Metastatic
tumor
A small percentage
of cancer cells may
survive and establish
a new tumor in another
part of the body.
Web sites about cancer:
http://outreach.mcb.harvard.edu/animations_S03.htm
• About mitosis:
http://highered.mcgrawhill.com/sites/0072437316/student_view0/chapter11/animations
.html
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Comparison of mitosis and meiosis
•MITOSIS
-OCCURS IN ALL SOMATIC CELLS
MEIOSIS
-OCCURS ONLY IN SEX CELLS:
GAMETES (ova or eggs and sperm)
-PRODUCES IDENTICAL COPIES
- PRODUCES VARIATION in traits
•OF PARENT CELL. Daughter cell has
•same number of chromosomes as parent.
-RESULTS IN 2 DIPLOID (2n) cells
complete number of chromosomes
•-ONE REPLICATION OF CHROMOSOMES
CELL FOLLOWED BY ONE CELL DIVISION
- Results in 4 haploid (n) cells
reduced number of chromosomes
- ONE REPLICATION OF CHROMO
SOMES FOLLOWED BY 2 CELL DIVISIONS
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
FACTORS THAT CONTRIBUTE TO GENETIC VARIATION
MUTATIONS
CROSSING OVER DURING PROPHASE I OF MEIOSIS
INDEPENDENT ASSORTMENT OF CHROMOSOMES during meiosis
RANDOM FERTILIZATION OF THE EGG BY SPERM
-
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Check these web sites:
• http://www.cellsalive.com
• http:/www.biology.arizona.edu/cell_bio/tutorials/cell_cycle/cell
s2.html
• http://www.loci.wisc.edu/outreach/bioclips/CDBio.html
• http://gslc.genetics.utah.edu/units/stemcells/whatissc
Play this game:
• http://science.nhmccd.edu/biol/bio1int.htm
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings