Transcript Cell - Images

Plasma Membrane
Structure and Function
Chapter 7
Image from: http://www.biologie.uni-hamburg.de/b-online/ge22/03.gif
Slide show modified from:
http://www.explorebiology.com/pptAP/2005/
http://facstaff.bloomu.edu/gdavis/links%20100.htm
FLUID MOSAIC MODEL
Animation from: http://www.sp.uconn.edu/~terry/images/anim/fluidmem.gif
Click here to
See Fluidity
Click here to See
FLUIDITY
1972- S.J. Singer and G. Nicolson propose membrane is a
“mosaic” of proteins and phospholipids that are constantly
moving and changing
More than just a barrier…
• Expanding our view of cell membrane
beyond just a phospholipid bilayer barrier
– phospholipids plus…
A membrane is a collage of different proteins
embedded in the fluid matrix of the lipid
bilayer
Membrane Proteins
• Proteins determine most of membrane’s specific
functions
– cell membrane & organelle membranes each have
unique collections of proteins
• Membrane proteins:
– peripheral proteins = loosely
bound to surface of membrane
– integral proteins = penetrate into
lipid bilayer, often completely
spanning the membrane =
transmembrane protein
Membrane Carbohydrates
• Attached to proteins (glycoproteins)
or lipids (glycolipids)
• Play a key role in cell-cell recognition
– ability of a cell to distinguish neighboring cells
from another
– important in organ &
tissue development
– basis for rejection of
foreign cells by
immune system
http://faculty.southwest.tn.edu/rburkett/GB1-osmosis.htm
Membranes provide a variety of cell
functions
Molecules need to move across
membranes in cells
OUT
waste
ammonia
salts
CO2
H2O
products
IN
food
carbohydrates
sugars,
proteins
amino acids
lipids
salts, O2, H2O
Image modiified from: http://www.accessexcellence.org/AB/GG/importProt.html
Cell Membranes are made of
PHOSPHOLIPIDS & PROTEINS
Amphipathic –
Molecules with
both hydrophilic
and hydrophobic regions
phosphate
hydrophilic
lipid
hydrophobic
Semi-permeable membrane
• Need to allow passage through the
membrane
• But need to control what gets in or out
– membrane needs to be semi-permeable
sugar
aa
lipid
H 2O
salt
NH3
So what makes a membrane semi permeable?
See a movie
PHOBIC TAILS in center determine
what can pass through
What molecules can get through directly?
Small non-polar molar molecules (O2 & CO2) and
hydrophobic molecules (fats & other lipids)
can slip directly through the phospholipid cell
membrane, but…
inside cell
NH3
lipid
O2
outside cell sugar aa
salt
H 2O
What about
other stuff?
Diffusion
• 2nd Law of Thermodynamics
governs biological systems
– Universe tends towards disorder
Animatioin from: http://www.biologycorner.com/resources/diffusion-animated.gif
• Diffusion
– movement from [higher]  [lower] concentration
Diffusion will also
happen across a
cell membrane as
long as there is a
difference in
concentration and
the membrane
will let the
molecule pass
through.
Example: DIFFUSION IN CELLS
http://facstaff.bloomu.edu/gdavis/links%20100.htm
O2 automatically moves from
HIGHER concentration (in lungs) to
LOWER concentration (in blood)
CO2 automatically moves from
HIGHER concentration (in blood)
to LOWER concentration (in lungs)
http://www.le.ac.uk/pa/teach/va/anatomy/case2/2_2.html
Diffusion of 2 solutes
• Each substance diffuses down its own
concentration gradient, independent of
concentration gradients of other
substances
What if cell needs to move a
AGAINST the
molecule _________
CONCENTRATION
GRADIENT?
_______________
(LOWER  HIGHER)
Cell example:
Want to put MORE glucose
into mitochondria when there is
already glucose in there
Image from: http://www.biologyclass.net/mitochondria.jpg
What if a cell needs to
LARGE or ______
POLAR
move _____
molecules
that can’t get
through the
membrane?
http://www.d.umn.edu/~sdowning/Membranes/membraneImages/jpegimages/diffusionmedium.jpg
What if cell needs to move
FAST
molecules really _______?
(can’t wait for it to diffuse)
Cell example:
Movement of
Na + & K+ ions
required to send
nerve signals
http://www.steve.gb.com/images/science/neuron.png
WAY to
Cells need a ____
HELP molecules across
____
cell membranes that
can’t go across by
_______
themselves
___________
Kidspiration by: Riedell
Facilitated diffusion
• Move from HIGH to LOW concentration with
aid of membrane transport proteins
– passive transport
– no energy needed
– facilitated = with help
Facilitated Diffusion
Animation from: http://bio.winona.edu/berg/ANIMTNS/facdifan.gif
Carrier Proteins
Grab molecule,
undergo conformational
change, flip to other side
Channel Proteins
Create passageway for
substances to pass
through
May be gated or not:
open in response to
chemical or electrical
signals
Animation from: http://www2.uic.edu/~myilma1/ionchannel.gif
Carriers and Channels are specific
inside cell
H2O
aa
sugar
NH3
salt
outside cell
. . . BUT STILL MOVES FROM [HIGHER] to [LOWER]
Active transport
Uses energy from ATP to move molecules
against concentration gradient
– Moves from [lower] → [higher]
– Uses protein pumps
OR
vesicles
Animations from:
http://academic.brooklyn.cuny.edu/biology/bio4fv/page/cell-movement.html
http://www.cat.cc.md.us/courses/bio141/lecguide/unit1/eustruct/images/sppump.gif
Active transport
SODIUM-POTASSIUM PUMP
Sets up difference in charge across membranes
Pumps 3 Na+ out
and 2 K+ in
Makes cells
more + outside
more - inside
See a movie
about Na+ - K+ pump
Animation from: http://www.cat.cc.md.us/courses/bio141/lecguide/unit1/eustruct/images/sppump.gif
Na+ - K+ pump sets up MEMBRANE
POTENTIAL
http://hyperphysics.phy-astr.gsu.edu/hbase/biology/nervecell.html#c2
BULK TRANSPORT
• ACTIVE transport
• Requires energy (ATP)
• Uses vesicles
Watch two video clips about
endo/exocytosis
endo/exocytosis
Animation from: http://www.emc.maricopa.edu/faculty/farabee/BIOBK/BioBooktransp.html
Endocytosis
phagocytosis
pinocytosis
receptor-mediated
endocytosis
“Cell eating”
large molecules;
whole cells
“Cell drinking”
Fluids;
Small molecules
triggered by
ligand signal
PHAGOCYTOSIS
Animation from: http://academic.brooklyn.cuny.edu/biology/bio4fv/page/cell-movement.html
“Cell eating”
takes in
large molecules;
whole cells
See phagocytosis
in action:
http://www.accs.net/users/kriel/chapter%20nine/
Example in cells:
WHITE BLOOD CELL ENGULFING
BACTERIA using Phagocytosis
SEE
PHAGOCYTOSIS
MOVIE
http://fig.cox.miami.edu/~cmallery/255/255ion/fig14x28.jpg
PINOCYTOSIS
“Cell drinking”
Takes in fluids;
Small molecules
http://student.ccbcmd.edu/~gkaiser/biotutorials/eustruct/pinocyt.html
Receptor-mediated Endocytosis
• Ligand-any molecule that binds to a receptor site
• Binding of ligands allows uptake of specific
molecules
Receptor-mediated Endocytosis
Example in cells: Cholesterol Uptake
Low density lipid proteins (LDL’s) bind cholesterol
and carry it in blood
LDL’s act as ligands binding
receptors on cell surface
and cholesterol is taken
into cell
http://academic.brooklyn.cuny.edu/biology/bio4fv/page/rectpr.htm
EXOCYTOSIS
• Active transport (requires ATP)
• Uses vesicles
• Releases substances to outside
INSULIN being released by pancreas cells using exocytosis
Video: http://www.southtexascollege.edu/tdehne/BC_ShockwaveAnimations/07SWF-TourOfTheCell/07-16-EndomembraneSystem.swf
GOLGI BODIES USE EXOCYTOSIS
Animation from: http://www.franklincollege.edu/bioweb/A&Pfiles/week04.html
See a Golgi movie
http://fig.cox.miami.edu/~cmallery/255/255ion/fig14x26.jpg
Transport summary
The Special Case of Water
Movement of water across
the cell membrane =
OSMOSIS
Osmosis is diffusion of water
• Water is very important, so we talk about
water separately
• Diffusion of water from high concentration
of water to low concentration of water
– across a
semi-permeable
membrane
– Passive
– Uses no energy
TONICITY- ability of a solution to cause
a cell to lose or gain water
• Refers to the concentration of SOLUTES
• Is a RELATIVE term, comparing two
different solutions
Solute-substance that is dissolved
Solvent- substance solute in dissolved in
Solution = solute + solvent
What if there is a difference in
concentration but solute
molecules can’t move across a
membrane?
WATER will move
until concentrations
reach equilibrium
Animation: http://www.ouhscphysio.org/humanphys/animations/osmosis1.swf
See an animation
Osmosis1
http://faculty.etsu.edu/currie/images/osmosis1.jpg
Animation
Concentration of water
• Direction of osmosis is determined by
comparing total solute concentrations
– Hypertonic - more solute, less water
– Hypotonic - less solute, more water
– Isotonic - equal solute, equal water
water
hypotonic
hypertonic
net movement of water
Solute concentration
Lower outside
than inside
Equal outside
and inside
Greater outside
than inside
HYPOTONIC
ISOTONIC
HYPERTONIC
What will happen to an animal cell
placed in different solutions?
Animation from: http://www.ouhscphysio.org/humanphys/animations/osmosis3.swf
OSMOSIS
See an animation
Osmosis3
Video
Choose Blood
Hypotonic link
HYPOTONIC:
Concentration outside cell is
________________
inside the cell
LESS THAN
More water enters than leaves cell so cell
will ___________________
swell and possibly burst
Animation from: http://www.ouhscphysio.org/humanphys/animations/osmosis4.swf
OSMOSIS
See an animation
OSMOSIS 4
HYPERTONIC: Concentration outside cell is
____________________
inside cell
GREATER THAN
More water leaves cell than enters
so cell ____________
shrinks
Video
Choose Blood
Hypertonic link
Remember:
Cells try to “maintain stable
internal conditions =
HOMEOSTASIS
____________________
http://bioweb.wku.edu/courses/biol121/Osmosis/Osmosis.asp
So an animal cell in
ISOTONIC conditions
stays same size
Water entering = water leaving
Video
Choose Blood Isotonic link
Animal cells
= CYTOLYSIS
= CRENATION
http://www.stchs.org/science/courses/sbioa/metenergy/bloodcells.gif
http://www.stchs.org/science/courses/sbioa/metenergy/aplantturgor.gif
Plant cells
Cell wall keeps
plant cell from bursting
= PLASMOLYSIS
Managing water balance
• Isotonic
– animal cell immersed in isotonic
solution
• blood cells in blood
• no net movement of water across
plasma membrane
• water flows across membrane, at
same rate in both directions
• volume of cell is stable
Osmosis…
.05 M
.03 M
Cell (compared to beaker)  hypertonic or hypotonic
Beaker (compared to cell)  hypertonic or hypotonic
Which way does the water flow?  in or out of cell
http://www.biology4kids.com/files/cell_vacuole.html
Loss of water from central vacuole= plants “wilt”
Vacuole full of water gives plant support
(turgor pressure)
Turgid = very firm
Flaccid = limp
HYPOTONIC
Sitting in the bathtub
makes your fingers
plump up and get “pruny”
Grocery stores spray
water on their veggies
to “plump them up”
If cells can’t maintain
“stable internal conditions” . . .
damage can result and cells can die.
http://www.the-aps.org/education/lot/cell/Quiz.htm
Drink salt water
ACTIVELY pump ions OUT;
Urinate less frequently
Gills ACTIVELY pump ions in;
Urinate frequently