Transcript Presentation

Saratov State University named
after N.G. Chernyshevsky
Specific optical
rotation indicatrices of
chitosan films
D.A. Rudenko and A.B. Shipovskaya
BRIEF ANNOTATION
The optical activity of chitosan films in the form of
polysalt (chitosan acetate) and polybase was studied. The
specific optical rotation [α] of all our films was negative.
The absolute values ​of [α] of polybasic chitosan films was
by an order of magnitude higher than that for
polysaltic films. A dependence of [α] on the orientation
angle of the sample relative to the direction of the
polarization vector of the incident light beam in the plane
perpendicular to this beam was established. Specific
optical rotationindicatrices of the chitosan films of both
chemical forms were plotted.
Structural formula of chitosan
0.515 í ì
H
H
HO
NH2
H
O
OH
CH2
H
H
O
HO
H
H
HO
H
CH2
OH
O
H
H
O
O
NH2
H
H
OH
CH2
H
CH2
OH
O
H
H
H
HO
CH3
H
CO
NH
H
HO
O
H
x
NH2
OH
H
-(1 4)-ãëèêî çèäí àÿ ñâÿçü
n-x-2
Î ñòàòî ê
D-ãëþ êî çàì èí à
Î ñòàòî ê
N-àöåòèë-D-ãëþ êî çàì èí à
Properties
 Biocompatibility with human tissues
 Biological and optical activity biodegradability
 Digestibility of the body, the properties of autologous
 Annually renewable raw material base
Objects of research:
We used a thin film of chitosan
in saline (C-) and the main (O-) forms
С-form
О-form
The reagent is a chemical reaction C- → O-form of chitosan: 1Н NaOH
The solvent used was 2% aqueous acetic acid
Research methods
The optical activity was recorded on two automatic
spectropolarimeters: PolAAr 3001 (Optical Activity Ltd,
England), within the wavelength range of  = 300–600 nm,
T = 22±2ºС. The experimental conditions were standard,
and the measurement error of the rotation angles did not
exceed 0.002o. The specific optical rotation []
(deg ml dm-1 g-1) was calculated by the following formula:
,
22  20 C
λ , íì
[ ]


ρ
where α is the measured angle of optical rotation of the film
sample, in degrees; is the optical path length, dm; and is the
density of the film material, g/cm-3
Curves ORD films of chitosan in the
C- and O-forms
С-form
O-form
Figure 1. ORD curves for CTS films in salt (1) and basic (2) form. The moisture content
of the film samples was 222 (1) and 121 wt.% (2). Vertical bars show the variation
range of [] values for different orientation angles of the film sample.
Taking into account the fact that chitosan film and on
the basis thereof characterized by structural and
compositional heterogeneity amount measuring α
performed over the entire surface of the film sample. The
above discussed experiments varying range of values ​[α] at
different orientation angles  film sample shows vertical
bars.
Angular dependence (indicatrix) specific optical rotation
film СTS the orientation angle  of the sample relative to
the direction of the polarization vector of the incident light
beam in a plane perpendicular to the given beam in polar
and Cartesian coordinates are shown below.
Indicatrices specific optical rotation
Polar coordinates
(а)
Cartesian coordinates
(b)
С-form
(c)
(d)
O-form
Figure 2. The dependence of the specific optical rotation [α] the angle  of rotation of the
sample polar (a, b) and Cartesian coordinates (c, d) films CTS in the form of polisoli
(acetate) (a, b), poliosnovaniya (in g) at α = 365 (1) 405 (2) 436 (3), 546 (4) and 589 nm (5).
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