Transcript Document

DETECTION AND
SURVEILLANCE
Sheila Grant
Department of Biological Engineering
UMC
University of Missouri
Goal
• Goal is to ensure that early and accurate
detection is available for important pathogens
and zoonotic pathogens in various
environments and deployment mechanisms
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Methods of Introduction
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Aerosol release
Food supply
Water supply
Direct infection
Direct exposure from
infected people/animals
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How to Protect?
Biological sensors
Field RT-PCR
Syndromic surveillance
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Integration of Surveillance Mechanisms
Introduction of FAD
Sensors: “Detect”
Syndromic: “Increase vigilance”
Detect?
yes
no
Field PCR: “Increased surveillance”
Provisional
containment
measures
implemented
yes
Detect?
no
Laboratory
confirmation
Continue surveillance
Implement response measures
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Sensor Development
1. Biological detection elements and transducer
system
2. Microfabrication
3. (Aerosol collection)
4. Signal processing, transmission, and
networking
5. Modeling
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Biological detection element and
transducer systems
Biosensor system
Biological Detectors
Transducers
+
=
• antibodies
•optical
• peptides
•acoustic wave
• receptors
•electrochemical
bioterrorist agents
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FRET Immunosensor
• measures the conformational changes that occurs
when antibodies bind to select agents
• technique can eliminate false positives since only
viable agents can elicit a
conformational
change.
lo
l1
l2
DD
Protein A
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Sensing Peptides
Mechanical:
Shear Horizontal-SAW (SH-SAW) biosensors will detect enzymes in an aqueous
solution. This device will detect a change in wave propagation speed as the targeted
enzyme in solution cleaves a specific peptide-construct, vastly increasing
specificity.
Optical:
Additionally, labeled peptide-constructs
can be immobilized to gold nanoparticles,
which effectively quenches
fluorescence. Upon interactions with
target enzymes, the peptide is cleaved and
fluorescence is enhanced.
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Ring resonator toxin sensor
using fluorescence method
Au nanoparticle
SNARE
Au nanoparticles spoil Q-factor
Upconversion is inhibited
Microsphere doped
with Erbium at the surface
980 nm laser
Upon SNARE cleavage,
Au particles are released
Upconversion is possible to detect
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Microfabrication and Nanotechnology
Nanoporous
waveguide materials
Peristaltic
Micro-pumps
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Signal detection on a chip
Anodic Bonding
between
the two
substrates
Output
Reservoir
Excitation Window
Detector
Waste Chamber
Light
Micro channel
with a Liquid
guide
Light
guide
Excitation source
core waveguide
Water
PDMS
Input
Reservoir
Analyte solution
being pumped in
Meandering Type
Nanoporous
Micro-mixer
Silica
Cross Detection
Detection using
using liquid
solid core
Inline
corewave-guide
wave-guide
(LCW)
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Integrated Fluorescence Assay on a Chip
Detector-covered
wall
Measurement
Flowcell
LASER
Reference
Diode
D
Donor
Diode
Acceptor
Diode
F1
F2
Short light pulses are generated by the laser and directed onto the sensor
fluorophore inside the flowcell.
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Future directions
• Real time detection
• Centralized data based system
• Modeling
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Acknowledgements
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Xudong (Sherman) Fan
Frank Feng
Shubhra Gangopadhyay
Kevin Gillis
Mark Haidekker
Susan Lever
Darcy Lichlyter
Graduate Students
• Shantanu Bhattacharya
• Rosalynn Manor
• Mary Pierce (now employed by MRI)
• Lisa Boettcher
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