Transcript 5 tcp/rer/3402/dgn/stroj - Assistance to Western Balkan Countries for

Regional Workshop 2: Improving Capacity for
Diagnosis of Fish and Molluscan Diseases
Banja Luka, Bosnia and Herzegovina, 20-24 October 2013,
Hotel Palas
National report on
Diagnosis of Fish
Diseases in Republic of
Macedonia
Activities done 2012/2013
 Transposition of EU legislation (dir.2006/88),
 Official controls
 Registration of fish farms (RBO)
Production data 2012
Species/tons
 Fish
Rainbow trout,
River trout,
Cyprinus carpio - common carp,
Hypopthalamichthys nobilis-bigheaded carp,
Silurus glanis - catfish, and
Ctenopharyngodon idella- grass carp
 Molluscs - no
 Species/farms- no addequate data for 2012
 Registration of fish farms/ aquaculture
production businesses (90 farms)
 Movement/transfers
There has been no export on live aquaculture
animals in the period (2010-2013);
 Importation of eggs/fry/live fish (2012)
 Live fish (for human consumption in kg) =45600 (Serbia,
Kosovo, Bulgaria)

Juveniles (for human consumption in kg) =12500 (Serbia,
Bulgaria)

Fresh fish (for human consumption in kg)= 231105 (Bulgaria,
Greece, Kosovo, Albania, Norway)

Fish eggs (kg)= 647 (Denmark, South Africa, Poland, USA,
Serbia)
Diseases recorded in the
country
Viral diseases (NONE yet)
Bacterial diseases (Furunculosis, Yersiniosis, Carp
Erythrodermatitis, Bacterial Gill Disease, Columnaris
disease, Bacterial cold water disease)
Parasitic diseases (Ichthyobodosis, Ichthyophthiriosis,
Chilodonellosis, Dactylogyrosis, Gyrodactylosis,
Botriocephalosis, Argulosis, Lerneosis, Ergasilosis)
 Fungal diseases (Saprolegniosis)
 Other health problems (Algal blooms, Asphyxia, Toxic
effluents)
Diagnostic capacity in the
country
 Aquatic animal diseases laboratory within country
(Faculty of veterinary medicine, Veterinary institute, Lab.
for bacteriology, Lab. for virology, Lab. for parasitology,
Lab. for serology and molecular diagnosis)
 All of the laboratories are involved in diagnostic,
research and educational activities
 Scope (diagnosis of animal diseases) and species
interest (….fish – common carp and rainbow trout)
Level of diagnostic
 Level of diagnostic:
 Level 1: Gross observation, general necropsy
(external, internal)
 Level II: Bacteriology, Parasitology, Mycology,
Histology
 Level III: Cell lines, Immunological tests, TEM,
Molecular techniques
Analysis of diagnostic capacity
 Bacterial diseases (clinical observation, gross pathology,
isolation of bacteria on BA, MCA and TSA, subculture,
Gram stain, Cat., Ox., API and Vitek 2)
 Parasitic diseases (clinical observation, gross pathology,
microscopy)
 Viral diseases (Ag-ELISA and PCR for selected diseases)
Surveillance of nationally listed diseases
Diseases that are covered with the current annual
order on animal health are:
 Viral hemorrhagic septicemia (VHS),
 Infectious pancreatic necrosis (IPN)
 Bacterial kidney disease (BKD),
 Spring viremia of carp (SVC), and
 Koi herpes virus (KHV),
 Diagnostic methods (Ag-ELISA for VHS, IPN, SVC;
PCR for KHV and qualitative immunochromatography
for BKD)
Surveillance of nationally listed diseases 2
 Sampling
for Viral hemorrhagic septicemia (VHS), samples in trout
shall be taken at least twice a year in early spring for
offspring and late autumn for parent flock;
 for Infectious pancreatic necrosis (IPN), examination shall
be carried out in June for offspring and in December for
parent flock;
 for Bacterial kidney disease (BKD) samples in trout shall be
taken in early spring and late autumn;
 for Spring viremia of carp samples (SVC), shall be taken in
early spring and
 for Herpes virus disease (KHV), samples shall be taken in
July/ August.

Surveillance of nationally listed diseases 3
 Results (all tested samples are negative)
Surveillance and monitoring of other pathogens
 Targeted pathogens and aquaculture species
NO
Study of abnormal mortality – upon
notification - mortality description
Name of fish
farm-details
of the case
Date
Mortality
Laboratory results
8 carp fish
farms at Tikves
lake
June 2011
200 tons of
all categories
Negative on listed diseases; Cause of
mortality was lack of oxygen due to
the increased population of algae in
the lake.
One rainbow
trout farm Kriva palanka
May 2011
24000
Negative on listed diseases; Cause of
juveniles and the increased mortality happened due
200 parents to construction work which lead to
clouding of the water causing
deposition of dirt on the on the gills of
the fish leading to suffocation.
HatcheryHidrobioloski
zavod, Ohrid
January
2012
100000
juveniles of
Ohrid trout
Negative on listed diseases; Cause of
the increased mortality happened due
to construction work which lead to
increase of levels of CO2 in the water
and suffocation of the aquaculture.
Conclusions and perspectives
 No accurate data on production,
 No accurate data on movement and transport,
 No authorisation of fish production businesses,
 Surveillance scheme not yet put in place,
 Diagnostic laboratory need to be improved;
Future needs
 Improvement of the laboratory for diagnostic of
aquaculture animals
 Implementation of new surveillance strategy
 Public awareness
 Development of guidelines, forms and instructions
for keeping records (production,
transport/movement, authorization…)
THANK YOU !