Transcript 9 tcp/rer/3402/dgn/zrnci - Assistance to Western Balkan Countries

Regional Workshop 2: Improving Capacity for
Diagnosis of Fish and Molluscan Diseases
Banja Luka, Bosnia and Herzegovina, 20-24 October 2013,
Hotel Palas
National reports overview of regional
status
Production data 2012
Most represented – carp and rainbow trout
Less – other cyprinid and salmonid species
and marine fish (Croatia, Montenegro, BiH)
 Molluscs
Mussels
Oysters
 Movement/transfers
 Between farms within country
 Importation of eggs/fry/live fish
Importation of market sized fish for human
consumption
Importation of fry for cultivation from neighboring
countries and European countries (Hungary, Italy,
Bulgaria etc)
Importation of eggs mainly from Denmark and USA
Diseases recorded in the
countries
 Viral diseases – IPNV in Croatia, Bosnia and
Herzegovina, Serbia
VHSV – single outbreak in Croatia
Bacterial diseases – very similar in all countries –
furunculosis, yersiniosis, BKD, flavobacteriosis,
carp erithrodermatitis, columnaris disease, motile
aeromonas septicaemia (MAS)
Marine fish species – vibriosis, tenacibaculosis
Parasitic diseases – marteiliosis in mussels in
Croatia and Montenegro; ectoparasites
 Fungal diseases – saprolegniosis
 Other health problems – Newly described diseases
RMS /strawberry disease
Diagnostic capacity in the
countries
 Aquatic animal diseases laboratory within country
All coutries some kind of NRL with different
responsibilities; research and educational institution
dealing with aquatic animal health
 Some are mostly educational and other research
Level of diagnostic
 Level of diagnostic:
 Level 1: Gross observation, general necropsy
(external, internal)
 Level II: Bacteriology, Parasitology,
Mycology, Histology
 Level III: Cell lines, Immunological tests, TEM,
Molecular techniques
 MOST COUNTRIES – LEVEL II
Analysis of diagnstic capacity
 List of diseases and diagnostic methods used
 List is more or less same
 VHS, IPN, SVC, KHV – clinical observation, detection
of gross changes, histological observation, isolation of
virus on cell culture, identification by ELISA, PCR,
 Furunculosis, yersiniosis, BKD, carp
erythrodermatitis, columnaris – Isolation of bacteria
on bacteriological media, identification of genus by
selective media and general properties, identification
of the species by API or any other kit for biochemical
identification
Surveillance of nationally listed diseases
 Diagnostic methods – VHS, IHNV, PVŠ, IPNV,
KHV, BKD – according to the level of lab capacity
 Sampling – organised by Competent Authority
 Results – presence of IPNV, VHSV, PVŠ not
diagnosed in last two years, BKD sporadically
Surveillance and monitoring of other
pathogens
 Targeted pathogens and aquaculture species –
 Croatia – bacteria V. anguillarum and Tenacibaculum
maritimum in sea bass, Yersinia ruckeri and
Flavobacterium psyhrophilum – antimicrobial resistance
 Eustrongyloides sp. – possible human health threat – no
programme of control until now
 RMS, MAS, ECV – regionally present - due to insufficient
funding no surveillance or monitoring programmes
Study of abnormal mortality
 Notification of abnormal mortalities in Macedonia,
available diagnostic methods performed – mortality
caused by abiotic factors
 Similar cases in fish farms/ open waters are notified
in other counties -
Conclusions and perspectives
 Decrease of aquaculture production in BiH
 Improving progress in harmonisation of legislative in
Serbia, Montenegro
 Unsufficent laboratory capacity in BiH, Montenegro
and Macedonia (they are not complying with
OIE/EU standards for diagnosis of listed viral
diseases)
 There are no diagnostic methods for crustacean
diseases developed in any country in the region
Future needs
 Education for insufficient laboratory methods
 Improvement of lab equipment and procedures
 Evaluation of diagnostic possibilities through
interlaboratory proficiency testing (maybe it is
possible to organise it regionally within this project)
 Implementation of diagnostic methods for other
species (crustacean)
 Regional networking