Transcript EPI DEMIC - Georgia Standards

EPI
DEMIC
A Simulated Spread of an
Infectious Disease
Today’s Task
Today you will work with a
partner to perform a lab
simulation based on a scenario in
which you will explore the
simulated spread of an infection
This activity will indicate the
infected persons and the origin
of the infectious disease
Make sure you have answered
pre-lab questions and turned
them in to your instructor
Procedures
-Begin by reading the
scenario and discussing it
with your group. Make
sure you understand the
problem.
Make some predictions
about who has the disease
-Gather the materials you
need or check the
materials provided at
your station
Procedures
-Each station should have:
--Permanent Marker
--Micro centrifuge or test tube racks which
holds 2 tubes per student
--1/4 section of full 96 well ELISA tray
--1 positive & negative control solution in
dropper bottle with a clean pipette for each bottle
--Paper towels
--Primary Antibody Solution in dropper bottle with
clean pipette
-- Secondary Antibody Solution in dropper bottle
with clean pipette
--Washing solution 100ml in washing bottle
--Color Reagent Solution
Procedures
-Gather the materials you
need or check the materials
provided at your station
Each Group member should have
--Safety Glasses
--1 empty centrifuge/test
tube
--1ml simulated body fluid
--Access to negative and
positive antigen solution
--1 plastic transfer pipette
Procedures
Lets get started
-First Label both of your tubes
with your initials
-Next place an S on one tube for
sharing and Me on the other tube
for the tube you will not be
sharing
-Transfer half of the simulated
body fluids to the empty tube with
the transfer pipette
-The Me tube should be placed in
the assigned rack and set aside
Procedures
Now it is time to share and
this is how you will do it-Prepare to share your body
fluids with 1 person in the
class by choosing someone who
is not near you
-When Instructor gives the
signal, mix your body fluids
with the chosen person’s fluids
in one of the S tubes by using
a transfer pipette to remove
from one S tube (you or your
partner’s) and add to the
other
Procedures
-Gently mix the body fluids
using the pipette by drawing all
fluid into the pipette and
pushing it back into the tube
about 5 times
-Use the transfer pipette to
remove ½ of the fluid and put it
back in the S tube you emptied
of you or your partner
-Record the name of the
student whose fluids you just
mixed in space 1 on the ELISA
GROUP DATA TABLE provided
Procedures
-When Instructor gives the signal
repeat the body fluid exchange
step with a different partner and
record that person’s name on the
table as number 2
-When Instructor gives the signal,
find a 3rd person to share your
body fluids with following the
same procedure and record their
name on the table in space 3
-Return to your station, place your
tube in the rack provided to
complete the simulated ELISA
WHO HAS IT????
• Now it’s time to find out
how many people are
infected
• To find our if you are
infected, you will become
a Biotechnologist and
perform an ELISA
• (Enzyme-Linked
ImmunoSorbent Assay)
• Follow directions on the
handout
Procedures
• Please note:
• Each group member acts as
a Biotechnologist to
perform the ELISA
• One assigned member from
each group will also act as
an Epidemiologist
• Report Hepatitis B research
and ELISA Data as a team