Transcript Where can we find Bacteria?

Culturing Yeast Cells on
Media
• Pre Lab Definitions:
• Petri Dish: A round, shallow dish used to grow
bacteria.
• Culture: To grow living organisms in a
prepared mixture of nutrients (media).
• Media: Substance containing nutrients needed
for cell growth.
• Agar: Jelly to which food has been added for
the growth of microbes.
• Pre Lab Definitions:
• Inoculate: To introduce a microbe to an
environment where it can grow.
• Sterile/Aseptic Technique: Laboratory
procedures used in handling cultures, media and
equipment that prevent contamination.
• Colony: A visible cluster or group of cells
growing on a solid medium.
• Pre Lab Definitions:
Safety Equipment:
• Lab Coat, Glasses, Gloves
Lab- Part 1: Serial Dilution of
Yeast Broth
1. Label test tubes 1, 2, 3, 4.
2. Add 9 mL of water to test tubes 1, 2, 3, 4.
4. Add 1 mL (20 drops) of yeast broth (from
the shake flask) to test tube 1. (mix)
5. Pipette 1 mL of solution from test tube 1 to
test tube 2. (mix)
6. Pipette 1 mL of solution from test tube 2 to
test tube 3. (mix)
Lab- Part 1: Serial Dilution of
Yeast Broth
7. Pipette 1 mL of solution from test
tube 3 to test tube 4. (mix)
Lab Part 2- Inoculation of media
with Yeast Cells
1. Label your petri dish with your group’s name and
number (on the underside of the dish).
2. Swirl your disposable loop in test tube 4.
3. Use the disposable loop to spread the solution on
the surface of the agar- being careful not to press
too hard!
4. Place your dish in the incubator.
5. The cells will incubate or grow at 30 degrees C for
24 hours.