Transcript Slide 1

About the Speaker:
Program in Emerging Infectious
Diseases (EID)
“Dendritic Cells: Matching Mice
and Men”
By Dr Florent Ginhoux
Principal Investigator, Singapore Immunology Network
(SIgN), Agency for Science, Technology and Research
(A*STAR)
Abstract :
Dendritic cells (DCs) are heterogeneous immune cells
crucial for both defense against pathogens and tolerance.
DC populations in mouse and human non-lymphoid tissues
can be separated into functionally different subsets, that
include human CD141high DCs, CD1c+ DCs and CD14+
DCs and murine CD103+ DCs and CD11b+ DCs. We
recently showed that human CD141high DCs share
common gene expression and functionality with mouse
tissue CD103+ DCs such as their cross-presentation
capacity (Haniffa, Immunity 2012). However, the precise
functional interspecies correlation of the remaining subsets
remains incompletely defined. Here, we show that murine
lung and gut lamina propria CD11b+ DC populations are
comprised of two ontogenically and functionally distinct cell
types, bona fide Flt3- and IRF4-dependent CD24+CD64DCs and contaminating CSF-1R-dependent CD24-CD64+
interstitial macrophages.Extending these findings to
humans, we find that CD1c+ DCs are homologues of
murine CD24+CD11b+ DCs, expressing IRF4 and
promoting Th17 responses through IL23α secretion. Our
data reveal heterogeneity in mouse CD11b+ DC
compartment and identify in mucosal tissues an IRF4expressing DC lineage specialized to instruct IL-17
responses in both mouse and human. The functional
alignment of human and mouse DC subsets is essential for
translating mouse in vivo findings to human primary DC
immunobiology.
All are welcome
Date : April 2, 2013 (Tuesday)
Time : 3.00 – 4.00 pm
Host : Dr Manoj Krishnan
Program in Emerging Infectious Diseases
Venue : Duke-NUS, Amphitheatre, 2nd Floor
Florent Ginhoux graduated in
Biochemistry from the University
Pierre et Marie CURIE, Paris VI
and obtained a Masters degree in
Advanced Studies in Immunology
from the Pasteur Institute, Paris.
Dr Ginhoux then started his PhD
in the Immunology Team of
GENETHON, Evry and obtained
his PhD in 2004 from the
University Pierre et Marie CURIE,
Paris VI. As a postdoctoral fellow,
Florent Ginhoux joined the
Laboratory of Miriam Merad in the
Mount Sinai School of Medicine
(MSSM), New York where he
studied the ontogeny and the
homeostasis of dendritic cell
populations.
In 2008 he became an Assistant
Professor in the Department of
Gene and Cell Medicine, MSSM
and member of the Immunology
Institute of MSSM. He joined SIgN
in March 2009 to lead a laboratory
focusing on the ontogeny and
function of dendritic cells and
macrophages.
Duke-NUS Graduate Medical School, 8 College Road, S169857. For more information, please visit our website www.duke-nus.edu.sg