gene transfer and genetic engineering
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Transcript gene transfer and genetic engineering
GENE TRANSFER AND
GENETIC ENGINEERING
CHAPTER 8
1
Gene Transfer
Vertical to offspring
Lateral (horizontal) to others in same generation
2
Bacterial Gene Transfer and
Recombinant DNA Technology
Transformation
– Griffith’s
experiment
3
Transformation
Mechanism
Significance
Recombination?
4
Transduction
Mechanism
Transducing phages:
p1, p22
Significance
Can be used in genetic mapping
http://jb.asm.org/cgi/reprint/174/23/7876
5
Conjugation
Mechanism
Conjugation pilus
Significance
Also used for mapping
6
Plasmids
F plasmid
(conjugation)
Resistance plasmids
Virulence plasmids
7
Plasmids (cont.)
Tumor-inducing plasmids
Agrobacterium tumefaciens and plants
Plasmids conferring novel catabolism
Degradation pathways on a plasmid
8
Genetic Engineering
Genetic fusions
reporter fusions
promoter fusions
9
Genetic Engineering
Protoplast fusion
electroporation
10
Genetic Engineering (cont.)
Gene
Amplification
Recombinant
DNA cloning
11
Applications
Medical
Industrial
Agricultural
Hybridomas
12
Risks and Benefits
Frankenstein?
Frankenfoods?
13
Recombinant DNA technology
Plasmids- autonomous replicating extrachromasomal
DNA
restriction enzymes
14
Viral technology- phage cloning
15
Homologous recombination
Used to make gene
knockouts in bacteria and
yeast
16
Transposons - jumping genes
17
Evidence for extensive
transfer of antibiotic
resistance genes in
Bacteroides sp.
http://mcb.illinois.edu/faculty/profile/abigails
18