Transcript Genetic Engineering

Genetic Engineering
We can use a process called gel
electrophoresis to separate the pieces
Figure 20.x1a Laboratory worker reviewing DNA band pattern
Gel Electrophoresis
• Moves DNA because it is negative
• Separates DNA fragments based on size
• The smaller the fragment the farther it will
move
• Can compare DNA from individuals
Gel Electrophoresis
Figure 20.17 DNA fingerprints from a murder case
Figure 20.9 Using restriction fragment patterns to distinguish DNA from different
alleles
Recombinant DNA
When a piece of
foreign DNA (in
purple) is added to a
bacterial plasmid (a
circular piece of DNA
in bacteria), the end
result is known as
recombinant DNA
How to make
recombinant
DNA: take a
gene (piece of
DNA) from a
human cell
and combine it
with a plasmid
from a bacteria
using
restriction
enzymes.
Recombinant DNA: making plants glow