Transcript Diapositive 1

Table S1 related to Figure S1: characterization of the bacteria isolated.
Macro and
Microscopic
observations
Gram
test
Isolate ID
Species
Pink colony, lactose +
Small rod-shaped
mobile bacteria
-
CEC15
Escherichia coli
% of
identity of
the 16S
rRNAs
Most closely
related strain
Other features
99
E. coli strain
KNUC9044
Identified as E. coli by
Api®20E
100
E. faecalis strain
HAMBI3057,
VYH243702
sodA+
Black colony,
esculetin- ferric
ions(+)
Non mobile cocci,
diplococci or short
chains
+
CEF15
Enterococcus
faecalis
White colony
Long non mobile rodshaped
+
CLI15
Lactobacillus
intestinalis
100
L. Intestinalis strain
TH4
White colony
Fine non mobile rodshaped spore-forming
bacteria
+
CCI15
Clostridium
innocuum
99
C. innocuum
strain 607145/2009
CFV15
Novel
Fusobacterium
spp.
94
Fusobacterium
varium JCM 6320
White colony
Small non mobile rodshaped bacteria
-
Each isolated bacterial strain was characterized by macroscopic and microscopic observations and Gram testing. Genomic DNA extracted from
individual isolates was subjected to PCR with universal primers GCclamp-U968 and L1401. The most similar bacterial type strain sequences
were identified using Ribosomal Database Project 16S rRNA gene sequences and BLASTn analysis. This 16S rRNA gene analysis identified the
Enterobacteriaceae colony isolated as E. coli, and this was confirmed by the Api®20E tool (20100, Biomerieux); the Enterococcus colony
isolated as E. faecalis confirmed by the specific sodA PCR test (Jamet et al., 2012); the Lactobacillus colony isolated as L. intestinalis; and the
two anaerobic bacteria as C. innocuum and a novel Fusobacterium spp.
Jamet E, Akary E, Poisson MA, Chamba JF, Bertrand X, Serror P. (2012). Prevalence and characterization of antibiotic resistant Enterococcus
faecalis in French cheeses. Food microbiology 31:191-198.