Transcript In vitro prebiotic effect of Zingiber officinale (ginger) on Lactobacillus

In vitro prebiotic effect of
Zingiber officinale (ginger)
on Lactobacillus
Group: C1
Ko – Locaylocay
Introduction
Ginger
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Zingiber officinale
underground rhizome - herbal medicinal part
Main components:
sesquiterpenoids, nonvolatile phenylpropanoids
(gingerol & zingerone), diarylheptanoids
Gingerol – antioxidant, antipyretic, antimicrobial,
antiemetic
Introduction
Prebiotic
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Non-digestible food ingredient – stimulate the
growth or activity of one or more bacteria in the
colon
Dietary fibers – polysaccharides, lignins &
oligosaccharides
Introduction
Lactobacillus
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Gram-positive, facultative anaerobe or strictly
aerobic bacteria
Lactic acid bacteria group
Normal flora of vagina, GIT & GUT
Significance of the Study
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Competitive exclusion principle
Protection against gastric disturbances
Preventive measure for colon cancer
Research Question
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Does ginger have prebiotic effect on Lactobacillus in
vitro?
Objectives
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General Objective:
To determine the prebiotic effect of ginger on
Lactobacillus in vitro.
Objectives
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Specific Objectives:
1. To determine whether ginger flesh extract can
up-regulate growth of Lactobacillus in in vitro
conditions.
2. To determine which ginger extract concentration
is the most effective in enhancing the growth of
Lactobacillus.
3. To determine the degree and significance of
growth up-regulation ginger flesh extracts exert on
Lactobacillus in vitro.
Methodology
Aqueous Ginger Flesh Extract
Preparation
Ginger Root
Washed, Peeled, Weighed, Chopped, Homogenized
Whatman number 3 filter paper, 2µm syringe filter
100 mL Ginger Extract
Aqueous Ginger Flesh Extract
Preparation
100 mL Ginger Extract
Eight serial 1:2 dilutions
(100, 50, 25, 12.5, 6.25, 3.13, 1.56, and 0.78%)
Label
Keep at 4°C until use
Stored at -20°C
Standardization of Broth Colony and
Turbidity Testing
Lactobacillus Casei
Control Group
Experimental Group
(8 Ginger Extracts)
MacFarland 0.5 barium sulfate standard;
Split each tube into 3 replicates
Incubate for 24 hours
Turbidity by Spectrophotometry:
550 nm
Total Bacterial Count
Pour plate method
1 mL aliquot of each tube
10 mL MSA Agar
Tilt & Rotate plates
Incubated at 37°C for 24 hours
Colony Count
Statistical Analysis
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ANOVA
In vitro prebiotic effects of
Zingiber officinale (ginger) on
Lactobacillus
Group: C1
Ko – Locaylocay