Transcript Bacillus Cereus Data

Man-made vs. Herbal
Remedies
By: Gillian Chandler
Question:
Are herbal remedies more effective in killing bacteria
than man-made medicines?
I chose to do this experiment because...
Research:
• Peppermint extract is commonly used to treat an upset
stomach or aid digestion
- It also has been proven to be antibacterial
• Dandelion root can help digestion and be used as a diuretic
• Penicillin is used to kill bacteria in a wide range of Grampositive bacterias
-Many bacterias have built up resistance to penicillin
• Erythromycin is an antibiotic used to treat certain bacterial
infections
Research (2):
• Escherichia coli (E. Coli)
 Gram negative
 bacterial infection
 causes food poisoning
• Bacillus cereus (B. Cereus)
 Gram positive
 bacterial infection
 causes food borne illness
Research (3):
"Gram" refer how a bacteria reacts to an initial strain
Gram positive
o has many thick layers of peptidoglycan (sugar protein
shell)
o Effect on antibiotics
Gram negative
o cell wall has only contain a few layers of peptidoglycan
o outer membrane that covers wall
o inner membrane
o Effect on antibiotics
Hypothesis:
If both man-made and herbal remedies are used
against bacteria, then the man-made ones will be
more effective.
Materials:
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1 molar dandelion root solution
1 molar peppermint solution
Distilled Water
E.Coli
Bacillus cereus
Penicillin
Erythromycin
20 Petri dishes
o tryptic soy agar
• Bunsen burner
• Tweezers
• Inoculation loop
Procedure:
• Place the gram negative bacteria in ten of the petri dishes,
and the gram positive bacteria in the other ten petri dishes
• Create the controls of water and blank discs, and place two
in each of the four control dishes (2 b. cereus 2 e. coli)M
• Make two dishes with four discs of dandelion root extract
with E. Coli, and likewise with B. Cereus
• Make two dishes with four discs of peppermint extract with
E. Coli, and likewise with B. Cereus
• Make two dishes with four discs of penicillin with E. Coli and
likewise with B. Cereus
• Make two dishes with four discs of erythromycin with E. Coli
and likewise with B. Cereus
• Allow the bacterias to incubate
• Measure the zone of inhibition
Data:
Bacillus Cereus Data
Data (2):
Escherichia Coli Data
Data
Standard Deviationstatistical test of how legitimate your data is
Discussion
My hypothesis: If man-made and herbal remedies are used
against bacteria, then the man-made ones will be more
effective.
My hypothesis was partially supported.
The Bacillus Cereus results proved my hypothesis true.
 the erythromycin had a greater zone of inhibition than
the natural remedies
The Escherichia Coli results proved my hypothesis false.
 the peppermint had a greater zone of inhibition than
the man-made remedies.
This is because...
Discussion (2):
Improvements:
-use different types of herbal remedies
-use stronger antibiotics
Thank You
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