Transcript How well does GSE work in treating contaminated drinking water?

How well does GSE work in
treating contaminated
drinking water?
Background
Information
• GSE is considered a • Quaternary
broad spectrum antiammonias kill
bacterial
microbes by
destroying their
• GSE is a quaternary
cellular membrane
ammonium
compound made
• Pure GSE has been
form grapefruit seed
proven effective in
and pulp extract
laboratories
Damaging Effects of
GSE
• GSE is very acidic and can be irritating
to eyes and mucous membrane surfaces
of the body
• Prolonged use can have damaging
effects on beneficial organisms in the
body
• Like any anti-microbial, over use could
eventually lead to increase in GSE
resistant forms of microorganisms
InitialM
odel
Contaminated Water
Undergoes
GSE Treatment
Time ?
Mixing ?
* Concentration of GSE ?
Clean
Drinking Water
Assumptions
• Contaminated water used contains
disease causing bacteria
• Medium used to grow cultures does
not inhibit growth of disease
causing bacteria
• Both pathogenic and nonpathogenic types of bacteria can be
killed with GSE
M
ethodsUsed
• Contaminated water from Greenlake was
collected
• Water was treated with differing amounts of
GSE solution 5, 10, 15, 20, 25, 30 drops
• The samples were stirred and aloud to sit for
1 min.
• Then a sterile swab was used inoculate 8
TSY plates with the different samples
• The plates were allowed to grow for 48 hours
and then a plate count of each was done
Data
Sample
Control
Appearance Many
yellow
and
white
colonies,
haze on
surface
Plate Count
(number of
colonies)
Tap
Water
5 drops
GSE
10
drops
GSE
15
drops
GSE
20 drops
GSE
25 drops
GSE
30
drops
GSE
Very
small
white
colonies
Yellow
and white
colonies,
haze on
surface
White
colonies
, haze
on
surface
Dull
gray
colony
and
slight
haze
Dull gray
colonies,
very
slight
haze
Clear, no
haze
Clear,
no
haze
32
4
1
6
0
0
Too
2
Many
To
Count
>300
W
hich water sample would you
want to drink?
30 drops
Tap Water
GSE
solution
Untreated
Lake Water
25 drops
GSE
solution
5 drops
20 drops
GSE
solution
GSE
solution
10 drops
GSE
solution
15 drops
GSE
solution
FinalClaim
GSE in its low concentration form is
effective in killing some bacteria found
in contaminated drinking water.
However, it must be used at almost
double the recommended dose for it
to be considered sufficiently effective.
Revised Model
Contaminated Water
Undergoes
GSE Treatment
Clean
Drinking Water
Time 1 min
Number of Pathogenic
Bacteria Needed
To Cause Illness ?
Mixing Quick Stir
* Concentration of GSE
25 drops are needed
to be effective
Types of Bacteria and Other Microbes
Killed By GSE ?
Number of Bacteria
Present After Treatment?
References
Citricidal Grape Fruit Seed Extract.
http://gfx.com/citricidal.htm
Citricidal Technical Data
http://nutriteam.com/tech.html
Grapefruits for Immune Defense
http://www.mend.net/immune/grapefruit.html
Puotinen. Natural Solutions to Drug-Resistant Infections.
http://www.wellbeingjournal.com/nat-solutions.html
Totora, Funke, Case. Introduction to Microbiology.