Transcript What are the three steps in PCR?

What are the three steps in
PCR?
What are the three steps in
PCR?
Denaturation
Hybridization of Primer
DNA replication
What is the enzyme used for
PCR?
What is the enzyme used for
PCR?
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DNA Polymerase from a
hyperthermophile
This is why the enzyme is not destroyed
at 94 degrees Celsius
What are introns?
What are introns?
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Introns are non-coding sequences
found in structural genes in eukaryotes
They are removed during splicing
What is reverse transcriptase?
What is reverse transcriptase?
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It is an enzyme that copies RNA into
DNA
It is produced by certain viruses
What are restriction enzymes?
What are restriction enzymes?
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They are enzymes that cut DNA at
specific base sequences
They are produced by bacteria
Many of them cut the DNA so that they
produce “sticky ends” or single stranded
ends
What are vectors used in
making recombinant DNA?
What are vectors used in
making recombinant DNA?
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Vectors are self replicating pieces of
DNA
They are used to get DNA into cells
Two commonly used vectors are
plasmids and bacteriophage
What are three techniques used
to get recombinant DNA into
cells?
What are three techniques used
to get recombinant DNA into
cells?
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Transformation
Electroporation
Microinjection (for eukaryotic cells)
What does RFLP stand for?
What does RFLP stand for?
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Restriction fragment length
polymorphism
It is often used in DNA fingerprinting
It requires gel electrophoresis which
separates DNA by size