Transcript What’s fueling genome evolution? (Understanding shared

What’s fueling genome evolution?
(Understanding shared genomic resources inprokaryotes)
• PI: Janet Siefert, Rice University; CO-PI’s: Yuiry Fofanov,
University of Houston; George Fox, University of Houston; Peter
Gogarten, U. Conn; Dr. Maia Larios-Sanz, Rice University; Dr.
Jason Raymond, Lawrence Livermore; Dr. Carlos Solis, Rice
University; Dr. Valeria Souza, UNAM; Mike Travisano, University
of Houston.
What’s fueling genome evolution?
(Understanding shared genomic resources in prokaryotes)
Shared Genome Resource
Selection
Functional Integration
We propose to investigate horizontal gene flow in bacteria by integrating genomic analysis, microbial ecology, and experimental
selection systems. Established and novel approaches performed by a diversely talented team of investigators will integrate results in
a check-and-balance system. Using three phylogenetically distinct lineages of bacteria, we will investigate and quantify the shared
genome resource available for transfer and the functional integration of that shared resource. The proposed research and
interdisciplinary training will initiate a comprehensive framework in which to view prokaryotic genome evolution.
Genome analysis
Framework of
HGT Estimates
Microbial Ecology
Experimental selection
Three organisms
Bacillus – gram positive, low G+C,
ubiquitous soil/water
Cyanobacteria – oxygen producers
E. coli – gram negative, protobacter,
mammalian gut
Agenda
1. Introduction to the overall project (Janet - 10 minutes)
2. Welcome Jason, quick bio (Jason - 5 minutes)
3. Introduction of Cuatro Cienegas (Valeria, Janet - 20 minutes, see attached
powerpoint and http://www.laventa.it/photogallery/4c-pozze/index.htm)
4. Return to project scheme and flesh out individual contributions, old and
new methodology ideas, discuss integration schemes, writing
assignments.(1 hour)
5. Grant issues: advisory committe, deadlines, efforts. (20 minutes)
Genome Analysis
Experimental Systems
S-Plots – Yuiry
Nested timing-Direction of
HGT
Unit of Selection
E.coli
Cyano
Bacillus
Pseudomonas reporter strains
Ecology
Bacillus – Homologous
recombination
E.coli
Virus complement
Grant Items
• Advirsory Board
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Ed DeLong
Ford Doolittle
Grauer
Jonathan Eisen
David Lynch
Jill Banfield
Dave Ward
Forrest Rohwer (Virus)
• Deadlines
• Writing Assignments