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Impact des mycorhizes sur la revégétation
et la succession végétale primaire dans un
écosysystème hostile
Martina Janoušková,
Institute of Botany
ASCR, Czech Republic
Dr. Diederik van Tuinen,
UMR INRA-1088; CNRS-5184;
U. de Bourgogne
INRA-CMSE, Plant-MicrobeEnvironment
Dijon France
Experimental design
PLANTS
Tripleurospermum inodorum
Calamagrostis epigejos
Sisymbrium loeselii
AM FUNGI
G. intraradices BEG140
G. intraradices BEG141
G. claroideum BEG96
PLANTS
Tripleurospermum inodorum
Calamagrostis epigejos
10 replicates
Harvest at 4 and 8 weeks after
inoculation
AM FUNGI
Non-inoculated
G. intraradices BEG140
G. claroideum BEG96
G. intraradices BEG140 + G. claroideum BEG96
ANALYSES
Fresh and dry weight
Level of colonisation by AM fungi (Trypan blue)
Alkaline phosphatase activity
Intensity of root colonisation by each fungus (PCR)
Expression of selected fungal genes of
G. intraradices (qPCR)
Why collaborate?
• Working on degraded
experimental sites.
• Big collection of AM fungi
isolated from degraded
environments.
• Developed molecular
tools to monitor fungal
gene expression in
plant roots, and to
detect the fungus in
the roots.
Results and future
• Use of the molecular
tools to monitor and
assess the fungal
fitness in field
conditions
• Selection of new
markers appropriated
for fungal efficiency
monitoring
Glomus intraradices genome sequencing project