Transcript crete_1_10_2016x

• Cancer
• Diabetes
• Obesity
• Depression
• Multiple sclerosis
• Crohn’s disease
and many more..
Why is it difficult?
Strange inheritance patterns- sporadic cases
Classification of disorders based on symptoms may be problematic
Environmental and stochastic factors
Olivia Corradin and Peter C Scacheri, Genome
Med, 2014
1) Gateway cloning
2 different haplotypes (e.g. obesity risk haplotype
and non-risk haplotype)
upstream of different reporter genes to
destination vector with TOL2 recombination sites
2) Injections
1-2 cell embryos, Tol2 transposase mRNA
3) Outcrosses
Observing the different patterns of reporter
activation
4) Knock down candidate enhancer-binding
proteins
Observe changes in reporter activation pattern
mRNA in-situ
hybridization for
candidate target gene
Compare patterns
Morpholino knock-down