Transcript Living up to your Nernst potential

https://thecrowuwb.wordpress.com/
Living up to your Nernst potential:
a graphical approach to electrochemical gradients
Gregory J. Crowther, Ph.D.
Tues., Feb. 9
2:30pm
Discovery 464
Net driving force
OUT
Membrane
potential
–∞
+∞
IN
Photo from: http://oregonstate.edu/microbiology/vegathurberlab/feature-stories/saudi-arabia-2015
Learn about coral
microbiomes from
Jesse Zaneveld
Student lunch:
Thursday, February 11th at 12:00 pm. Email [email protected] for details.
Research Talk: Thursday, February 11th at 2:00 pm in UW2-307
Title: Exploring the Microbial Wilderness: Theory, Tools, and Applications from Chronic Disease to Wildlife
Conservation
Teaching demonstration: Friday, February 12th at 11:00am in UWBB 205
Title: Interactive Demo: Key Perspectives on Microbial Communities in Health and Disease
David Sommerfeld, Ph.D.
BBio 351 – February 1, 2016
Today’s outline
• Finish endocrinology (adrenal disorders, thyroid paper)
• Start Chapter 16: Reproduction!
• More endocrinology, really
• Return of the hypothalamic-pituitary axis
•
H. Hasuwa et al., Science 2013
• Examples of / exceptions to negative feedback
•
Ovarian/uterine cycle
• LH surge
• Birth control
Reproduction, homeostasis, negative feedback
Reproduction: connection with endocrinology
F. H. Martini et al. (2014)
Gamete and embryo development
Sherwood Figures 16-3 and 16-4c
Female (cat) reproductive anatomy
biology.clc.uc.edu/fankhauser/
Male reproductive anatomy
CAT
SHEEP
biology.clc.uc.edu/fankhauser/
fau.pearlashes.com/anatomy/
dissections/reproductive/testis10.jpg
The ovarian & uterine cycles
Day
0
10th Martini Figure 28-24
(like Sherwood Figure 16-16)
Day
14
Day
28
The ovarian & uterine cycles
Day
0
10th Martini Figure 28-24
Day
14
Day
28
10th Martini Figure 28-24 (like Sherwood Figure 16-17)
What’s “wrong”
with this picture?
Sherwood Figure 16-18a
Tracking the cycle to promote or avoid pregnancy
• Urine test – which hormone should be tracked?
Birth control: “the pill”
• What does it contain?
• How is pregnancy avoided?
• Prevent ovulation?
• Prevent fertilization?
• Prevent implantation?
Picture: JournalTimes.com
faculty.washington.edu/crowther/Misc/Songs/
New research on the hypothalamic-pituitary-gonadal axis:
H. Hasuwa et al., Science 341: 71-73, 2013
• Title: “miR200b and miR429 function in mouse ovulation
and are essential for female fertility”
• Review: hypothalamic-pituitary-gonadal axis
• Main question: exactly does GnRH promote LH secretion?
• Review: control of gene expression
What are microRNAs (miRNAs)?
Stefanie S. Jeffrey, Nature Biotechnology 2008
Genetic tools/tricks: reporter assay to see how
miR-200b & miR-429 affect Zeb1 expression
Hasuwa et al. (2013), Figure S13
Genetic tools/tricks: overexpressing Zeb
Google Images search results;
Hasuwa et al. (2013), Figure 3B-C
Literature-reading tip:
figure (and table) titles often collectively tell the story!
• Fig. 1. Infertility phenotype of miR-DKO mice.
• Fig. 2. Pituitary defects in miR-DKO female mice.
• Fig. 3. Lhb expression is regulated by ZEB1 as a
potential target of miR-200b and miR-429.
Fig. 1. Infertility phenotype of miR-DKO mice.
Hasuwa et al. (2013), Figure 1A
Fig. 1. Infertility phenotype of miR-DKO mice.
Hasuwa et al. (2013), Figure 1C-F
Fig. 2. Pituitary defects in miR-DKO mice.
Hasuwa et al. (2013), Figure 2A-D
Fig. 2. Pituitary defects in miR-DKO mice.
Hasuwa et al. (2013), Figure 2E-H
Fig. 3. Lhb expression is regulated by ZEB1 as a
potential target of miR-200b and miR-429.
Hasuwa et al. (2013), Figure 3D-E
Diagram to summarize main findings?
What questions could be researched next?