Transcript TPJ_4609_sm_FigureS3

TaqI
EcoRV
SlSERK1 Probe
HindIII
EcoRV
Kb
BglII
SlSERK3B Probe
Kb
10
8
6
5
4
10
8
SlSERK1
6
SlSERK3A 5
SlSERK3B
4
3
3
2
2
1.5
1.5
1
1
Figure S3. DNA-blot analysis of SlSERK family members in tomato cv. Motelle. Genomic DNA, 5 µg, was digested with the
indicated restriction enzymes and DNA blots were prepared according to standard protocols. The blots were hybridized with a
32P labeled probe in 50% (v/v) formamide at 42ºC. Final blot washes for SlSERK3B and SlSERK1 were performed in 1X SSC,
0.1% SDS at 42ºC and at 65ºC, respectively. The SlSERK3B probe consists of a cDNA fragment spanning nucleotides 12191536 in the kinase domain. The SlSERK1 probe consists of a cDNA fragment spanning nucleotides 318-610 in LRR3-LRR5
region. In agreement with the tomato genome analysis, the band patterns with SlSERK3B probe revealed the presence of 3
members of SERK genes in tomato. The SlSERK1 probe detected only fragments expected to correspond to SERK1.
Arrows point to the SlSERK member with the matching gene color.