Transcript Moderately Repetitive Sequences Code for rRNA Structure and

Eukaryotic Genome
& Gene Expression
Genome Structure and Postranscriptional Events
Pre-mRNA
Splicing
Polyadenylation
Capping
pppGp
mRNA
AAAAAAA250
Eukaryotic Transcription & Translation are
Compartmentalized
Categories of Genes and Non-Coding Sequences
• Class I genes
 rDNA encodes rRNA
 Moderately repetitive sequences
 Transcribed by RNA polymerase I
• Class II genes
 mRNA (protein encoding) and snRNA
 Unique sequences
 Transcribed by RNA polymerase II
• Class III genes
 tRNA, 5S rRNA, U6 snRNA
 Moderately repetitive sequences
 Transcribed by RNA polymerase III
• Non-coding regions
 Repetitive elements
 Transposable elements (some transcribed)
 Pseudogenes
Moderately Repetitive Sequences Code for rRNA
Structure and Transcription of a Eukaryotic Gene
The Globin Gene Family
Differential Expression in the Globin Gene Family
Processing the Ends of Eukaryotic Pre-mRNA
The Spliceosome, an RNA Splicing Machine
Alternative Splicing Results in Different mRNAs
and Proteins
Points of Regulation of Gene Expression
Eukaryotic Transcription
Initiation
Eukaryotic Transcription Initiation
Coordinating Gene
Expression
Protein-DNA Interactions
Local Remodeling of Chromatin for Transcription
A Barr Body in the Nucleus of a Female Cell
A Model for X Chromosome Inactivation
The Proteasome Breaks Down Proteins
Comparison of Prokaryotic and Eukaryotic Genes
and Genomes
Comparison of E.coli and Yeast Genomes
C. elegans Genes Essential to Multicellularity
Arabidopsis Genes Unique to Plants
Comparison of Rice and Arabidopsis Genomes