Transcript GENETICS – BIO 300

REVIEW I: TRANSMISSION GENETICS
exam 1: W.10.11
chapters 1 – 5
1. genetics & the organism
2. patterns of inheritance
3. chromosomal basis of inheritance
4. eukaryotic chromosome mapping
by recombination
5. genetics of bacteria & their viruses
CH01: GENETICS & THE ORGANISM
basic concepts
genetic material does what 3 things ?
patterns of transmission + meiosis = ?
what are genes & what are they doing
?
variation
continuous & discontinuous
P = G + E + G*E
norms of reaction
NORMS OF REACTION
PHENOTYPE
G
G+E
G
1
EE1
E2
E1
G*E
E2
E1
E2
E1
E2
ENVIRONMENT
G
2
CH01: GENETICS & THE ORGANISM
quantitative methods
artificial selection
classical methods
mutagenesis, selection screens
map, function, molecular
biology
complications
expressivity, penetrance
polygeny, pleiotropy
GENETIC COMPONENT
0.35
FREQUENCY
0.30
0.25
0.20
0.15
0.10
0.05
0.00
 x
 x
PHENOTYPE
CH01: GENETICS & THE ORGANISM
key terms on p. 24
no assigned
questions
CH02: PATTERNS OF INHERITANCE
basic concepts
what evidence for the existence of genes ?
single genes & discrete phenotypes
what are Mendel’s laws ?
how are sex-linked genes inherited ?
Mendelian analysis
controlled breeding & terminology
1 gene: monohybrid & test cross ratios
2 genes: dihybrid & test cross ratios
CH02: PATTERNS OF INHERITANCE
Mendelian analysis… continued
methods: Punnet, long & short trees
formulae to predict outcomes
hypothesis testing with 2
binomial expansion to predict outcomes
Poisson distribution to predict
outcomes
sex-linked inheritance
pedigree analysis
CH02: PATTERNS OF INHERITANCE
key terms on p. 59
all questions beginning on p.
62
CH03: CHROMOSOMAL BASIS OF INHERITANCE
basic concepts
how do we know genes are on chromosomes ?
how are genes arranged on chromosomes ?
are chromosomes only genes ?
how is chromosome number maintained ?
what are the chromosomal bases of Mendel’s laws?
chromosome theory of inheritance
evidence supporting theory
chromosome structure & organization
CH03: CHROMOSOMAL BASIS OF INHERITANCE
mitosis & meiosis
stages & chromosome movement
differences & similarities of
processes
relationships to gene transmission
fungal genetics introduced
CHROMOSOMES & INHERITANCE
CH03: CHROMOSOMAL BASIS OF INHERITANCE
key terms on p. 107
questions 1, 2, 5-10, 12, 16, 18-20,
22, 25-30, 32, 33, 40 beginning on p.
109
CH04: CHROMOSOME MAPPING & RECOMBINATION
basic concepts
genes mapped by recombination frequencies
distances are additive
one recombination event can interfere with
another
linkage
linked vs unlinked inheritance
with 2 test for linkage
linkage maps
3 point test cross
CH04: CHROMOSOME MAPPING & RECOMBINATION
linkage maps (continued)
accuracy in mapping by recombination
mapping function based on Poisson disctribution
single meioses in fungi
 ordered analysis
 unordered analysis
CH04: CHROMOSOME MAPPING & RECOMBINATION
key terms on p. 137-8
questions 1-30 beginning on p.
141
CH05: GENETICS OF BACTERIA & THEIR VIRUSES
basic concepts
how can bacterial genomes recombine ?
how can viral genomes recombine ?
how do bacterial & viral genomes interact ?
how can we map genomes of these organisms ?
working with microorganisms
growing them
mutants
CH05: GENETICS OF BACTERIA & THEIR VIRUSES
conjugation in bacteria
first evidence, F plasmids
F-, F+ & Hfr strains
interrupted mating experiments… gene
order
high resolution mapping
F’ plasmids
transformation in bacteria
co-transformation mapping
CH05: GENETICS OF BACTERIA & THEIR VIRUSES
phage genetics
phage cross
recombination in phage
transduction in bacteria
life cycles
generalized transduction, lytic cycle
specialized transduction, lysogenic cycle
co-transduction mapping
CH05: GENETICS OF BACTERIA & THEIR VIRUSES
key terms on p. 176-7
questions 1-10, 12-32
beginning on p. 179