Transcript Genetika Molekuler (3)

Genetika Molekuler (3)
Sutarno
• Lecture #2 Notes (Yeast Genetics)
• LECTURE 2: MUTANT ISOLATION STRATEGIES,
BASIC GENETICS TESTS
• If we want to do genetics the most important step is
ISOLATING MUTANTS
• Genetics is a conceptual science, (people like Mendel
and Morgan and McClintock didn’t know what a gene
was, but they were still able to identify and map genes).
• Mutants can be isolated with no prior knowledge of
what their specific function is.
• The goal...to identify ALL the genes that affect a
given process
• otherwise it's like trying to solve a puzzle without having
all the pieces
• will take multiple selections to achieve this
• One choice that has to be made: