Transcript Engineering E.Coli to see Light

Engineering E.Coli to see Light
Voigt CA et. al. 2005
Light Engineering Imaging using E.Coli
System
Stimulus
Sensor
Signal
Transducer
Effector
Output
Stimulus
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Red Light
Highly defined edges
Two-dimensional image
Sensor
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Phytochrome
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Membrane bound extracellular sensor
Intracellular regulator
Fusion protein: phytochrome and
EnvZ histidine kinase domain
Extracellular
sensor
Intracellular
regulator
Photoreceptor Expression
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PCB not produced in E.Coli
Introduction of PCB synthesis genes
Converts haem into PCB
Transduction Mechanism
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Red light represses EnvZ autophosphorylation
Output
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OmpR-P activates promoter linked
to reporter gene
lacZ reporter produce black
compound
OmpR-P dependent
ompC promoter
System Revisited
Review
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Created novel genetic circuit
High definition film
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(~100 megapixels per square inch)
lacZ expression as a function of
light intensity / wavelength
Transfer function
Something for the team
to think about!!
Light Canon
Characterisation
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Macro
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Resolution
Micro
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Transfer function
SigB activity as a function of:
Intensity
 Wavelength
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