Transcript Previously in Bio308

Terms and Players
Work on as class comes in– groups, alone, books, notes
Francois, Elliot, Dani, Kristen, Kelly:
What are
the basic monomeric subunits of DNA
bonds between subunits
direction of strand formation
description of double helix
Spencer, Byron, Christina, Rachel, Anders
What are
the basic monomeric subunits of RNA
bonds between subunits
organization of strand
Today:
Preview for today
Synaptic signaling requires protein receptors
and peptide- derived signals
How do you make a protein?
Protein building 101: Transcription and Translation
Questions
What is the central dogma?
How would a neuropeptide get made
(in general terms)?
What are the basic parts of DNA, RNA,
and proteins?
What is the difference between hnRNA,
mRNA and tRNA?
Synthesis overview
What carries the information?
Polymerization of nucleotides
Genes
‘ The entire nucleic acid sequence necessary for the
synthesis of a functional polypeptide’
MCB, p285
Components of a Eukaryotic Gene
What are the ‘parts’ or regions of a gene?
How are they organized?
Do genes always encode RNAs?
Do genes always encode RNAs that encode proteins?
Questions
How does RNA polymerase work and what does it make?
How does it know where to start and stop?
How does a ribosome work and what does it make?
How does it know where to start and stop?
If the DNA in every cell in your body is the
same why don't your adipose (fat)
cells secrete epinephrine?
If the DNA contains all of the information why doesn't
the ribosome just 'read' it?
Why have intermediate steps?
Looking at RNA ECB2 7.1
Making a cytosolic protein:
Step 1--transcribe
Genes encode proteins
Why use an RNA step?
Major steps in process: Initiation, Elongation and Termination
animation
Initiation
DNA-RNA interactions
DNA
Template strand
Complementary RNA strand
DNA/RNA
Hybrid
Watching
Transcription
EBC2 7.2
Adapted from Life; Purves 6thed
http://www.stolaf.edu/people/giannini/flashanimat/molgenetics/transcription.swf
http://www.johnkyrk.com/DNAtranscription.html
Check out
Synthesis overview