Transcript PowerPoint 演示文稿

Molecular Biology: Lecture 1 (11 Feb 04):
Introduction to the Course
Molecular Biology 2004
《分子生物学》
Objectives, Course Mechanics &
Assessment
For WHU Undergraduates (72 class hours)
Spring semester, 2004
Lecturers & class division
of the Spring Courses in Molecular Biology
Class
Nr of
students
116
Bio Base
Biotech Base
Biosciences Ca 120
Pharmacy
Lab course
Ca. 70
Schedule
Lecturer TA
Mon 3-4
Wed 1-2
Mon 3-4
Wed 1-2
Mon 3-4
Wed 1-2
郭德银
张 翼
朱 应
毛歆
吴莹
徐静
 Obligatory course for biology students
 Intended for sophomores and juniors of
College of Life Science, WHU
 Prerequisite: General biology, Biochemistry
 The course is taught in English (but
occasionally in Chinese to help
understanding)
 Text book: P.C. Turner et al. , Instant Notes
in Molecular Biology, Bios Scientific
Publishers Limited, 2001
Why this course in English?
 Tradition: in English for WHU BioStudents
since Spring 2002
 English is indeed an International Scientific
Language: reading cutting-edge literature,
study abroad, scientific meeting, discussion with
foreign colleagues etc
 Good and bad sides
Objectives of the Course:
（1）Basic knowledge: learn actively and
grasp firmly;
（2）Ability to apply your knowledge: active
thinking and imagination, use your knowledge
agily to solve theoretical and practical
problems;
（3）Language ability: understand Molecualr
Biology in English without sig. difficulty；
（4）Learning skills: attitude to learning and
exams, teaching and learning efficiency.
How to achieve the above aims?
(Course mechanics)
（1）Multimedium teaching: More figures and
diagrams to visualize；talk in the simple way to
learn and remember，stimulation of thinking.
（2）Course web page: teaching notes, on-line
discussion; other internet resources
(http://www.lib.whu.edu.cn or
http://202.114.65.51/fzjx/fzswx/)
How to achieve the above aims?
（3）Courseware preparation: one topic each to
train the student’s ability in independent work。
（4）Group discussion (8-10 people): study
atmosphere, teamwork, mutual help and
inspiration, shaping your character and moral.
（5）In-class question: to encourage active
thinking and vivify class atmosphere.
How to achieve the above aims?
（6）Quiz and exams: 6 quizes in addition to the
final examination.
（5）Tutorship: Course webpage, timely
tutorship mainly by TA
Courware (ppt) preparation:
1. 10 points in total : 4+3+3
2. basic points (4): integral and systemic
3. Extra content and accuracy (3)
4. Original figures and animation (3)
Group study :
10 points in total (together with attendance)
8-10 in one group with a group leader & a vice leader
At least 10 times, 1 hour each time
1. Recorders in turn, making the minutes in
detail (time, place, participants, questions and
answers etc)
2. Help each other (Raise and answer
questions in group)
3. Write down the unanswered questions at
the end of the discussion
Rules in class :
1. No wisper between the neighbours
2. Mobile phone should be turned off
4. Ask question by first raising your hand
Evaluation system:
1. Modular Quizes - 30% (30 points in 100 system)
2. Final exam: - 50%
3. Topic presentation (ppt format) - 10%
4. Group study and attendance: - 10%
5. Bonus points: 1-10 points
Ahaa, your final results might exceed 100 points!!!
考试规则
考试的目的是督促学习与检查教学效果, 但考场的表现反映一个
人的基本素质和诚信意识
（1）不离开自己的座位。（2）合上书本和笔记。（3）个人只
能看和写自己的试卷, 不得左顾右看或交头接耳（4）老师宣布停
的时候，所有学生必须放下自己的笔，目视前方。（5）老师宣
布收试卷时，试卷必须快速由每排的一端传到另一端.
课堂小考: 如果违反以上任何一条规则，第一次违规者，
本次考试成绩为零，第二次违规者必须重修这们课。缺
席不补考, 本次成绩为零，但如果提前请假可以得当次
考试的最低分。
期末考试：任何作弊者，一经发现，立刻取消考试资格，
重修此课。
Attitudes to the examinations
As the instructor
• Not as teaching goals but to stimulate the study
enthusiasm，help to summarize and master the
knowledge
• The test questions should render elicitation for the
study, not for giving difficulty to students
As a student
• Not the aim of your study but the means to help study
• Use the exams to cjeck and solidify your knwledge
• Enjoy your exams, but not suffer
What is Molecular Biology?
Molecular biology seeks to explain the relationships
between the structure and function of biological
molecules and how these relationships contribute to the
operation and control of biochemical processes. --Turner et al.
Molecular biology is the study of genes and their
activities at the molecular level, including transcription,
translation, DNA replication, recombination and
translocation.
--- Robert Weaver
The Core of Molecular Biology:
 The macromolecules and macromolecular
complexes of DNA, RNA and protein
 The processes of replication, transcription and
translation and their regulations
 Manipulation of macromolecules, technologies
central to modern biosciences
The hierarchy of living world:
Biological organizations at various levels
Biosphere (the sum of all Earth’s ecosystems)
Ecosystem (biological community+environment)
Biological community (different populations in same area)
Population (localized group of organisms of same species)
Organisms (Units of life)
Organ systems
Organs
Tissue
Cells (1-100 m,Units of organisms)
Organelles (ribosomes ca. 20 nm)
Macromolecule complex
Molecules (atoms bonded chemically)
Atoms (Å, chemical building
blocks of all matter)
The relationship of Molecular Biology with other biological
subjects
Pathology, clinic medicine
Preventive medicine
Medical
Sciences etc
Macro
(outdoor)
Life
Sciences
Biology
Ecology, Behavior
Evolution
Systematics
Micro
(indoor)
Crop sciences
Agri.
Sciences
Biochem
Cell
Biology
Mol
Genetics
Molecular
Microbiol
Biology
Dev
Biol
Animal husbandry
Immunol
Veterinary
Others
Mol
Neurobiol
Focus of various disciplines:
General biology: Anatomy, metabolism & systematics of
living organisms (plants, animals, microbes).
Microbiology/Virology: properties of microbes/viruses.
Biochemistry: chemical nature and metabolism of
biomolecules (macro- or small).
Biophysics: Physical properties of biomolecules
Molecular/Genetics: transfer of genetic information
between generations (genotype and phenotype).
Cell biology: Structure & function of cells and
organelles.
Molecular biology: Molecular nature of macromolecules
and gene replication & expression inside cells and
manipulation in vitro.
Text book
& course contents
Text book：P.C. Turner et al. 2000. Instant Notes in Molecular
Biology，2000 edition，Bios Scientific Publishers Limited.
Uniqueness: Concise and easily assimilated; 19 sections with
70 topics; Each subsection (topic) contains a “Key notes” panel in
addition to the main part of the topic; Sections or topics rather
independent; Cross-reference.
Reference book： Benjamin Lewin. 2000. Gene VII， Electronic
version 2000 – one of the mainstream textbooks.
Week 1-2 :
Week 3-5:
Week 6-8:
Week 9-11:
Week 12-13:
Week 14-16:
Week 17-18:
Cells and macromolecules (protein & nucleic acids)
DNA—chromosome, replication, repair
Transcription and its regulation in prokaryotes
RNA—transcription and regulation, RNA processing in eukaryotes
Ribosome, tRNA, mRNA and protein translation
DNA recombination, genetic engineering
Genomics and post-genomics
周次
Lecture Topics (Quizz in the week in red)
第一周 (Section A)
Course introduction, Cells and macromolecules
第二周 (Section B+C)
蛋白结构 (Protein Structure), 核酸的性质 (Properties of nucleic acids)
第三周 (Section D)
Genetic material in brief, Prokaryotic and eukaryotic chromosome structure
第四周 (Section E)
DNA复制 (DNA replication)
第五周 (Section F)
DNA损伤、修复和重组 (DNA damage, repair and recombination)
第六周 (K-Q brief)
General introduction to gene, genetic codon and gene expression
第七周 (Section K)
原核转录 (Transcription in prokaryotes)
第八周 (Section L)
原核转录调控 (Regulation of transcription in prokaryotes)
第九周 (Section M)
真核转录(Transcription in eukaryotes)
第十周 (Section N)
真核转录调控(Regulation of transcription in eukaryotes)
第十一周 (Section O)
RNA加工和核糖核蛋白 (RNA processing and RNPs
第十二周 (Section P)
Ribosome, tRNA, and mRNA
第十三周 (Section Q)
蛋白翻译 (protein synthesis)
第十四周(SectionGH
Gene technology in brief, Genetic manipulation & Cloning vectors
第十五周 (Section I)
基因文库和筛选 (Gene libraries and screening)
第十六周(Section J)
分析和使用克隆的DNA (Analysis and uses of cloned DNA)
第十七周
Genomics and post-genomics
第十八周
Review of course content and preparation for final exam
Course statements
As your instructor：
I ask myself
Do my best to prepare and give lectures. I trust students and
believe all of you can learn in an efficient way.
I ask my students
1. You should trust your own capability to learn this course
whatsoever how you have learnt other courses
2. Preview can make you more efficient
3. Efficiently use your class time, ask how much you have leant
(understood and mastered), any progress?
4. Improve your study through discussion and quiz.
5. Don’t be pretentious when you learn well and don’t be
disappointed, either, when you have difficulty (In this case,
believe yourself you can catch up)
I am very happy to be your course instructor
Let’s work together to make this course
a great experience in your life
I am looking forward to having
a wonderful semester with all of you
Hope that you all enjoy the course
I am sure I will