Transcript Diapositiva 1

THE OPTIMIZATION OF ULTRASOUND-ASSISTED
EXTRACTION OF PHENOLIC COMPOUNDS FROM
EUPHORBIA CYPARISSIAS (EUPHORBIACEAE)
Octavian Tudorel Olaru1, Mihaela Petrescu1, Andreea Sălăgean1, Valentina Anuța1,
1“Carol
Davila” University of Medicine and Pharmacy, Bucharest, Romania
Objectives
E. cyparissias contains polyphenolic compounds with anti-inflammatory
and cytotoxic activity. Therefore, we intend to optimize the phenolic
compounds extraction parameters by applying the Box-Behnken design.
Material and methods
In order to study the individual and combined effects of important
variables and the optimization of the extraction process, a factorial plan
reduced by three variables at three levels was used. The variables studied
were the solvent, the temperature, the extraction time and the ratio
solvent/plant material.
The response was represented by the total amount of phenolic compounds,
and was determined by Folin Ciocalteu spectrophotometric method. The
results were expressed as gallic acid equivalents (mg/g).
The validation of the model was performed by phenolic compounds
extraction at optimized parameters, followed by comparison of the
experimental values ​of the dependent variable with those obtained
theoretically by applying the mathematical model.
Fig 1. Euphorbia cyparissias.
Results
The optimum extraction solvent was ethanol 50%. Analysis of variance showed that from all of the variables, only the
temperature and the ratio solvent/plant material significantly influence the process (p = 0.001 and p <0.0001), whereas the
influence of the extraction time was not significant (p = 0.19). Interactions between independent variables were found
statistically insignificant.
a
b
c
Fig 2. Influence of time, temperature and solvent-plant material ratio on the extraction process of the phenolic
compounds from E. cyparissias; a – actual factor: solvent/plant material ratio; b – actual factor: time; c –
actual factor: temperature.
Conclusions
By applying the polynomial equation, which describe the
factors effect on the dependent variable, and response surface
methodology the optimum values ​of the independent variables
were determined. These are: a temperature of 75° C, extraction
time of 14 minutes and the extraction ratio solvent:plant
material of 45,8:1 (volume/weight).
References
1.Olaru OT, Şeremet OC, Petrescu M, Sălăgean A, Velescu BȘ, Nițulescu GM.
Toxicity evaluation and polyphenols assessment of some extracts from
indigenous Euphorbia species. Rom. J. Biopys. 2014; 24 (1): 1-9;
2.Chandrika LP, Fereidoon S. Optimization of extraction of phenolic compounds
from wheat using response surface methodology, Food Chem. 2005; 93 (1):
47-56.