Transcript 2. Cell Transport Mechanisms

The Plasma Membrane -
Gateway to the Cell
1
Types of Transport
Across Cell
Membranes
2
Simple Diffusion
• Requires NO
energy
• Molecules
move from
area of HIGH
to LOW
concentration
3
DIFFUSION
Diffusion is a
PASSIVE process
which means no
energy is used to
make the
molecules move,
they have a
natural KINETIC
ENERGY
4
Diffusion of Liquids
5
Diffusion through a
Membrane
Cell membrane
Solute moves DOWN concentration gradient (HIGH to
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LOW)
Osmosis
• Diffusion of water
across a
membrane
• Moves from HIGH
water potential
(low solute) to
LOW water
potential (high
solute)
Diffusion across a membrane
Semipermeable
membrane
7
Diffusion of H2O Across A
Membrane
High H2O potential
Low solute concentration
Low H2O potential
8
High solute concentration
Aquaporins
• Water Channels
• Protein pores used during
OSMOSIS
WATER
MOLECULES
9
Cell in Isotonic Solution
10% NaCL
90% H2O
ENVIRONMENT
CELL
10% NaCL
90% H2O
NO NET
MOVEMENT
What is the direction of water movement?
equilibrium
The cell is at _______________.
10
Cell in Hypotonic Solution
10% NaCL
90% H2O
CELL
20% NaCL
80% H2O
What is the direction of water movement?
11
Cell in Hypertonic Solution
15% NaCL
85% H2O
ENVIRONMENT
CELL
5% NaCL
95% H2O
What is the direction of water movement?
12
Cells in Solutions
13
Isotonic Solution
NO NET
MOVEMENT OF
H2O (equal amounts
entering & leaving)
Hypotonic
Solution
CYTOLYSIS
Hypertonic
Solution
PLASMOLYSIS
14
Cytolysis & Plasmolysis
Cytolysis
Plasmolysis
15
Osmosis in Red Blood Cells
Isotonic
Hypotonic
Hypertonic
16
What Happens to Blood Cells?
17
hypotonic
hypertonic
isotonic
hypertonic
isotonic
hypotonic
18
Three Forms of Transport Across the Membrane
19
Passive Transport
Simple Diffusion
 Doesn’t require energy
 Moves high to low
concentration
 Example: Oxygen or
water diffusing into a
cell and carbon dioxide
diffusing out.
20
Passive Transport
Facilitated diffusion
Doesn’t require energy
Uses transport
proteins to move high to
low concentration
Examples: Glucose or
amino acids moving from
blood into a cell.
21
Proteins Are Critical to
Membrane Function
22
Types of Transport Proteins
• Channel proteins are embedded
in the cell membrane & have a
pore for materials to cross
• Carrier proteins can change
shape to move material from
one side of the membrane to
the other
23
Facilitated Diffusion
Molecules will randomly move through
the pores in Channel Proteins.
24
Facilitated Diffusion
• Some Carrier
proteins do not
extend through
the membrane.
• They bond and
drag molecules
through the lipid
bilayer and
release them on
the opposite side.
25
Carrier Proteins
• Other carrier
proteins
change shape
to move
materials
across the cell
membrane
26
Active Transport
Requires energy or
ATP
Moves materials from
LOW to HIGH
concentration
AGAINST
concentration gradient
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Active transport
Examples: Pumping
Na+ (sodium ions)
out and K+
(potassium ions) in
against strong
concentration
gradients.
Called Na+-K+ Pump
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Sodium-Potassium Pump
3 Na+ pumped in for every 2 K+ pumped
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out; creates a membrane potential
Moving the “Big Stuff”
Exocytosis
- moving
things
out.
Molecules are moved out of the cell by vesicles that fuse
with the plasma membrane.
This is how many hormones are secreted and how nerve
30
cells communicate with one another.
Exocytosis
Exocytic
vesicle
immediately
after fusion
with plasma
membrane.
31
Moving the “Big Stuff”
Large molecules move materials into the cell by
one of three forms of endocytosis.
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Pinocytosis
Most common form of endocytosis.
Takes in dissolved molecules as a vesicle
33 .
Pinocytosis
• Cell forms an
invagination
• Materials
dissolve in
water to be
brought into cell
• Called “Cell
Drinking”
34
Example of Pinocytosis
pinocytic vesicles forming
mature transport vesicle
Transport across a capillary cell (blue).
35
Receptor-Mediated Endocytosis
Some integral proteins have receptors
on their surface to recognize & take in
hormones, cholesterol, etc.
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Receptor-Mediated Endocytosis
37
38
Endocytosis – Phagocytosis
Used to engulf large particles such as
food, bacteria, etc. into vesicles
Called “Cell Eating”
39
40
Phagocytosis About to Occur
41
Phagocytosis
- Capture
of a Yeast
Cell (yellow)
by
Membrane
Extensions
of an
Immune
System Cell
(blue)
42
Exocytosis
The opposite of endocytosis is exocytosis. Large
molecules that are manufactured in the cell are
released through the cell membrane.
Inside Cell
Cell environment
43