Transcript campbell biology in focus

CAMPBELL BIOLOGY IN FOCUS
Urry • Cain • Wasserman • Minorsky • Jackson • Reece
14
Gene Expression:
From Gene to Protein
Questions prepared by
Brad Stith, University of Colorado Denver
Louise Paquin, McDaniel College
© 2014 Pearson Education, Inc.
The final product of an expressed gene can be
which of the following? Also note the function
of each.
A. mRNA
B. tRNA
C. rRNA
D. polypeptide
© 2014 Pearson Education, Inc.
The final product of an expressed gene can be
which of the following? Also note the function
of each.
A. mRNA
B. tRNA
C. rRNA
D. polypeptide
© 2014 Pearson Education, Inc.
Of the following, which pertain to replication,
transcription, modification, or translation? Each can
be used once or more than once.
origin
stop codon
peptide
P site
GTP
amino acid synthetase
© 2014 Pearson Education, Inc.
anticodon
promoter
terminator sequence
tRNA
spliceosome
The template strand of a given gene includes the
sequence 3-G C C A C G T A T C A G-5
A. What is the sequence of the nontemplate
strand?
B. What is the mRNA sequence made?
For each one, be sure to indicate 5 and 3 ends.
© 2014 Pearson Education, Inc.
Which of the following is the best example of gene
expression?
A. A frog adapts to variation in its environmental
temperature.
B. Mouse fur color results from pigment formed by
gene-encoded enzymes.
C. DNA is replicated during the S phase of the cell
cycle.
D. The percent of A versus a alleles in a population is
altered by natural selection.
E. Mutation alters the sequence of a region of DNA.
© 2014 Pearson Education, Inc.
Which of the following is the best example of gene
expression?
A. A frog adapts to variation in its environmental
temperature.
B. Mouse fur color results from pigment formed by
gene-encoded enzymes.
C. DNA is replicated during the S phase of the cell
cycle.
D. The percent of A versus a alleles in a population is
altered by natural selection.
E. Mutation alters the sequence of a region of DNA.
© 2014 Pearson Education, Inc.
Give some examples that demonstrate the ability of
genes from one species to be expressed in a different
species. This is possible because of which property of
the genetic code?
© 2014 Pearson Education, Inc.
Describe what is occurring at each of the stages of
transcription (1, 2, and 3 in the figure). How does each
stage move the process forward?
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What components form the transcription complex at
a eukaryotic promoter?
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How and where are modifications made to
eukaryotic mRNA before it goes to the cytosol?
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What is represented at each of the lettered sites,
and what occurs at each of these sites during
translation?
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On this model of a tRNA molecule, identify each of
the following:
A. 5 and 3 ends
B. hydrogen bonds
C. amino acid attachment site
D. anticodon loop
© 2014 Pearson Education, Inc.
1. Aminoacyl-tRNA synthetase is an enzyme
whose function is to ________.
2. To do so, it binds a molecule of amino acid, and
two other molecules: ________ and ________.
3. After dephosphorylation, which two molecules
are joined by a covalent bond?
4. For the appropriate tRNA to be joined to the
amino acid, what must occur?
© 2014 Pearson Education, Inc.