Transcript Q01to05
Questions 1 -5 General Introductory Stuff 1. Overview of Metabolism • 15 4 6 11 186 Which of the following is an ANABOLIC reaction which occurs in humans? A. Net fixation of carbon dioxide into carbohydrate ANABOLIC BUT NOT IN HUMANS B. Hydrolysis of DNA into nucleotides CATABOLIC C. Proteolysis CATABOLIC D. Glycogenolysis CATABOLIC E. Lipogenesis ANABOLIC 2. ATP • Which statement is most CORRECT? A. 13 10 179 11 7 B. C. 50 g in a person, turnover 65 kg/day 1 kg of human tissue, on average, contains somewhere between 0.5 and 5 mg ATP In a healthy cell, the [ATP] is always much less than the [ADP] ATP = 4.8, ADP = 0.2, AMP in uM The total adenine nucleotide pool ([ATP] + [ADP] + [AMP]) in cells is about 5 mM ATP = 4.8, ADP = 0.2, AMP in uM D. ATP can be produced in the mitochondria of liver cells and transported in the blood for use by the muscle ATP doesn’t move out of cells E. At room temperature, a 5 mM solution of ATP will completely hydrolyse into ADP and phosphate within 1 minute. ATP is stable 3. General FA oxidation • Which statement about fatty acid oxidation is CORRECT? A. 11 186 11 11 2 Carnitine is a protein embedded in the cell membrane that allows fatty acids to enter from the bloodstream carnitine small molecule, transport across inner mito membrane B. Fatty acids are covalently attached to Coenzyme A during the fatty acid oxidation FAD/NAD catalysed oxidation reactions is on FA-CoA C. The oxidation reactions involving FAD/NAD occur only in the cytoplasm everything is in the mitochondria D. E. Fatty acids attached to Coenzyme A can move freely across the mitochondrial membrane CoA is the ultimate trapper! Carnitine is consumed (two carbons at a time) during fatty acid oxidation carnitine recycled – passes back into cytoplasm 4. Compartmentation • Which process occurs in the CYTOPLASM? A. Conversion of fatty acyl-CoA to acetyl-CoA ATP fatty acid oxidation – mitochondrial 16 156 6 16 23 B. Conversion of acetyl-CoA to malonyl-CoA Fatty acid synthesis - Cytoplasm C. Conversion of pyruvate to acetyl-CoA pyruvate dehydrogenase – mitochondrial D. Conversion of oxaloacetate and acetyl-CoA to citrate Krebs Cycle – mitochondrial E. Conversion of acetyl-CoA into ketone bodies ketone body formation – liver mitochondrial 5. General Krebs Cycle • Which description of the operation of the Krebs Cycle is MOST CORRECT? A. 7 14 32 144 21 not directly. but GTP made! The cycle turns acetyl-CoA into ATP B. The pathway is located in both the cytoplasm and the mitochondria only matrix and inner mito memb C. The cycle reacts fuel molecules with oxygen to produce carbon dioxide oxygen doesn’t come in ‘till electron transport acetyl CoA in, CoA and lots of Hs out D. The cycle generates CoA and NADH E. Most of the ATP in the cell is made directly by enzymes of the Krebs Cycle by substrate level phosphorylation. most ATP made by oxidative phosphorylation