Transcript Transcription and Translation

Transcription &
Translation
Protein Synthesis
Biology 12
Genes and Proteins


Proteins become structures
such as filaments in muscle
tissue, and enzymes that
control chemical reactions
Sequences of nucleotides in
DNA contain information
that is used in the
production of proteins



Genes direct the production of proteins
that determine the phenotypical
characteristics of organisms.
Genes also direct the production of other
physiologically essential proteins such as
antibodies and hormones.
Proteins drive cellular processes such as
metabolism; determining physical
characteristics and producing genetic
disorders by their absence or presence in
an altered form.
The Central Dogma


An organism’s genome is housed within the
nucleus. Proteins are synthesized outside the
nucleus, in the cytoplasm, on ribosomes.
Since information for protein synthesis is
specified by DNA (called the one gene-one
polypeptide hypothesis), and DNA is not able
to exist outside the nucleus, a problem exists
as to how the blueprint of life is brought to the
ribosomes.
SO…How does a cell
make proteins
anyway?
There are two important
nucleic acids involved in this
process...
DNA
RNA
Let’s Review DNA & RNA




DNA: deoxyribonucleic
acid--contains sugar
deoxyribose.
DNA is double
stranded.
DNA contains bases
adenine, guanine,
cytosine, and thymine.
DNA never leaves the
nucleus.




RNA: ribonucleic acid-contains sugar ribose.
RNA is single
stranded.
RNA contains bases
adenine, guanine,
cytosine and uracil.
RNA can leave the
nucleus.
Ameoba Sisters- DNA vs RNA
There are
three types of RNA

mRNA: messenger RNA
mRNA
tRNA: transfer RNA
rRNA: ribosomal RNA
tRNA
towing
Types of RNA
Genetic information copied from
DNA is transferred to 3 types
of RNA:
Messenger RNA: mRNA
Copy of information in DNA that is
brought to the ribosome and
translated into protein by tRNA &
rRNA
Varies in length , the longer the gene the
longer the mRNA>
Transfer RNA: tRNA
Brings the amino acid to the ribosome
that mRNA coded for.
Ribosomal RNA: rRNA
Most of the RNA in cells is associated
with structures known as ribosomes,
the protein factories of the cells.
Provides the construction site for the
assembly of polypeptides.
It is the site of translation where
genetic information brought by mRNA
is translated into actual proteins.
Transcription & Translation
Overall process of protein
synthesis
transcription
DNA
transcription
Occurs in the
nucleus
translation
RNA
translation
Protein
Occurs in the
cytoplasm
Ameoba Sisters – Protein Synthesis
The Connection Between Genes
and Proteins
Hank again!
Nucleic acids carry information in their nucleotide sequence.
Proteins carry information in their amino acid sequence.
To get from DNA (in nucleic acid language) to protein
(in amino acid language) requires two steps.
1. Transcription- a DNA strand provides a template for
the synthesis of a complementary RNA strand.
This molecule is called mRNA (messenger RNA).
DNA is too valuable to be allowed to exit the nucleus. This
could lead to the death of the cell and possible the
Death of the organism.
- use of mRNA provides protection for the
Genetic information contained in DNA.
- more protein can be made simultaneously
because many mRNA copies of a gene can be
made than if one strand of DNA left the nucleus.
- each mRNA can be translated many times.
mRNA delivers the encoded genetic
material to the ribosomes.
The ribosomes translate the message
into polypeptide chains, which are
processed into proteins.
This entire sequence is described as
the Central Dogma of Molecular
Genetics, first stated by Francis Crick
in 1958.
Central Dogma

In nucleus


Produced in nucleus
Travels to cytoplasm

Produced in cytoplasm
Transcription vs Translation
Transcription involves the copying of the
information in DNA into mRNA.
(copy from one medium to another- think
of a medical or legal stenographer)
Translation involves ribosomes using the
Messenger RNA as a blueprint to synthesize
a protein composed of amino acids.
(converting into a different language, think English
to French)
Transcription
Definition: Transcription
Transcription
Nucleus
Location
DNA
Template
(What is read)
To change DNA into a form that
can make a protein
Purpose
Messenger RNA
(mRNA)
Outcome
(End result)
Definition: Translation
Translation
Location
Cytoplasm (by ribosome)
Template
(What is read)
mRNA
Purpose
Amino acids assembled in particular
order to make a protein
Outcome
(End result)
Protein (polypeptide)
Transcription occurs in 3 steps:


Initiation, Elongation and Termination
Initiation:
RNA polymerase binds to the DNA at
a specific site known as a promotor.
DNA:
RNA:
A T G C A A
U A C G U U
The RNA transcript is known as
elongation.
After the RNA polymerase passes the end
of the gene, it stops transcribing which is termination.
Transcription : ‘to copy’
Initiation:
 RNA polymerase binds to DNA at ‘promoter’
 untwists the double helix 10 to 20 bases at a
time
Elongation:
 RNA polymerase builds mRNA
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
From DNA 3’ end
Uses complimentary base pairing

Remember: thymine (T) is replaced by uracil (U)
Termination:

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
RNA polymerase reaches end of gene.
Stops transcribing
Double helix reforms as mRNA molecule peels
away.
End Result:
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

mRNA breaks away from DNA
mRNA exits nucleus
If there is a high demand for a
protein, the cell can have several RNA
polymerases transcribing the gene at the same
time to produce several mRNA’s.
First, DNA unzips itself...

DNA unzips
itself, exposing
free nitrogen
bases.
First, DNA unzips itself...

DNA unzips
itself, exposing
free nitrogen
bases.
First, DNA unzips itself...

DNA unzips
itself, exposing
free nitrogen
bases.
First, DNA unzips itself...

DNA unzips
itself, exposing
free nitrogen
bases.
First, DNA unzips itself...

When DNA
unzips itself
(exposing free
nitrogen
bases)…
Next, mRNA is made...
mRNA matches
with free DNA
nitrogen bases in a
complementary
fashion
 (mRNA is made
from the DNA
template)

Next, mRNA is made...


mRNA matches
with free DNA
nitrogen bases in a
complementary
fashion
(mRNA is made
from the DNA
template)
Next, mRNA is made...
mRNA matches
with free DNA
nitrogen bases in
a complementary
fashion
 (mRNA is made
from the DNA
template)

Next, mRNA is made...
mRNA matches
with free DNA
nitrogen bases in
a complementary
fashion
 (mRNA is made
from the DNA
template)

Next, mRNA is made...
mRNA matches
with free DNA
nitrogen bases in
a complementary
fashion
 (mRNA is made
from the DNA
template)

Next, mRNA is made...
mRNA matches
with free DNA
nitrogen bases in
a complementary
fashion
 (mRNA is made
from the DNA
template)

Next, mRNA is made...
mRNA matches
with free DNA
nitrogen bases in
a complementary
fashion
 (mRNA is made
from the DNA
template)

Next, mRNA is made...
mRNA matches
with free DNA
nitrogen bases in
a complementary
fashion
 (mRNA is made
from the DNA
template)

Next, mRNA is made...
mRNA matches
with free DNA
nitrogen bases in
a complementary
fashion
 (mRNA is made
from the DNA
template)

Next, mRNA is made...
mRNA matches
with free DNA
nitrogen bases in
a complementary
fashion
 (mRNA is made
from the DNA
template)

Next, mRNA is made...
mRNA matches
with free DNA
nitrogen bases in
a complementary
fashion
 (mRNA is made
from the DNA
template)

Next, mRNA is made...
mRNA matches
with free DNA
nitrogen bases in
a complementary
fashion
 (mRNA is made
from the DNA
template)

Next, mRNA is made...
mRNA matches
with free DNA
nitrogen bases in
a complementary
fashion
 (mRNA is made
from the DNA
template)

Next, mRNA is made...
mRNA matches
with free DNA
nitrogen bases in
a complementary
fashion
 (mRNA is made
from the DNA
template)

Next, mRNA is made...
mRNA matches
with free DNA
nitrogen bases in
a complementary
fashion
 (mRNA is made
from the DNA
template)

Next, mRNA is made...
mRNA matches
with free DNA
nitrogen bases in
a complementary
fashion
 (mRNA is made
from the DNA
template)

Next, mRNA is made...
mRNA matches
with free DNA
nitrogen bases in
a complementary
fashion
 (mRNA is made
from the DNA
template)

Next, mRNA is made...
mRNA matches
with free DNA
nitrogen bases in
a complementary
fashion
 (mRNA is made
from the DNA
template)

Translation: ‘new language’
Initiation:
 Ribosome binds at a specific sequence on
the mRNA.
 The ribosome moves along the mRNA three
nucleotides at a time. This is called a codon.
Each set of three (a codon) codes for an
amino acid. Why?
There are only 4 bases but 20 amino acids.
41 = 4 (1 base=1 acid) 42 = 16 43 = 64
One code
two codes three codes
The codon AUG not only codes for the amino acid
Methionine, but it also indicates the start of a
translation.
Some amino acids are coded for by two or more
codons but a given codon ALWAYS only codes
for one amino acid.
GAA and GAG both code for glutamic acid,
but never mean any other amino acid.
here
mRNA tries to talk to the
ribosomes…

mRNA leaves the I have a message
you! It’s
nucleus and travels forfrom
DNA!
to the cytoplasm,
where the
ribosomes are
located.
Once there, mRNA
meets up with the
ribosomes
What does
he want now?
It’s always
something!
mRNA tries to talk to the ribosomes…but is
unsuccessful.

However, the
ribosomes cannot
understand the
message mRNA is
carrying.
Why don’t they
get it???
@%$!!
Why can’t we tell
what he’s saying?
We need a
translator!
tRNA Saves the Day!
Methionine
The boss will NOT
be happy about
this...
We won’t work until
we know what to do!
Where is that
translator?
Looks like trouble
for this cell…
I’d better help!
Now the cell
can make a
protein!
tRNA: Transfer RNA
Methionine
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Chemically, tRNA is
clover-leaf shaped.
At one end, it
carries an amino
acid.
At the other end, it
has a three letter
code known as an
anticodon.
Anticodon? What’s that?


This anticodon is the
complement to the
codons contained
within mRNA.
Can you find the
mRNA complement to
the anticodon on
tRNA?
Methionine
Elongation:
Ribosome moves along mRNA
 From mRNA 5’ end
 3 nucleotides of mRNA = codon = amino acid
 The “interpreter” tRNA delivers the proper complimentary base
to the ribosome. Anticodons are blocks of 3 tDNA bases that
actually attach to the correct protein.
 The anticodon( tRNA) binds by complimentary base pairing to the
nucleotides of the codon.
 Example: if the codon on a mRNA is UUU,
a tRNA with an AAA anticodon will bind to it.
The ribosome links adjacent amino acids with a peptide bond, causing
the amino acid to let go of it tRNA.
The finished protein has a sequence of amino acids that have been
determined by the mRNA base sequence which has been translated
by the tRNA.

The
Whole
Picture
Next amino
acid to be
added to
polypeptide
Growing
polypeptide
tRNA
mRNA


The ribosome then adds each amino acid
and the polypeptide chain is elongated.
Elongation occurs until a stop signal occurs.
Termination:
 Ribosome reaches stop codon
 Stops translating
End Result:
 Ribosome falls off mRNA
 Protein (polypeptide chain) is released
Start and Stop Codons
Start Codon:
 Begins translation

AUG (universal start codon)


ALSO Codes for methionine (Met)
Sometimes GUG or UUG
Stop Codon:
 Ends translation

UGA, UAA, UAG
Third letter
Codon Chart
Example

DNA template:
3’ TAC ACA CGG AAT GGG TAA AAA ACT 5’

Complimentary DNA


mRNA codon


Read from DNA template (start reading at 3’)
tRNA anticodon


Read from DNA template (start reading at 3’)
Read from mRNA
Amino Acids (protein)

Read from mRNA
ReviewFrom
DNA to
Protein
RNA types

mRNA

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

Messenger RNA
End product of
transcription
Takes message from
DNA into cytoplasm
Used by ribosome to
make protein

tRNA



Transfer RNA
Delivers amino acid
to ribosome
rRNA


Ribosomal RNA
Helps form and
maintain ribosomes
Transcription/Translation
Transcription
 Purpose:
 To make mRNA
from DNA
 Location:
 Nucleus
Translation
 Purpose:
 To make a
specific protein
from mRNA
 Location:
 Cytoplasm
(ribosome)
Stop vs. Start Codon
Start Codon
 mRNA code
 Tells ribosome to
begin translation
 Example:
 AUG
Also codes for
methionine
And: UUG, GUG


Stop Codon
 mRNA code
 Stops translation of
that specific amino
acid chain
 Examples:
 UAA, UAG, UGA
Transcribe to mRNA

DNA:
GGA TCA GGT CCA GGC AAT
TTA GCA TGC CCC AA

*mRNA*:
CCU AGU CCA GGU CCG UUA
AAU CGU ACG GGG UU
Translate to Amino Acids

mRNA sequence divided into codons:
GGC AUG GGA CAU UAU UUU GCC
CGU UGU GGU GGG GCG UGA

*Protein translation*:
Gly Met(start) Gly His Tyr Phe Ala
Arg Cys Gly Gly Ala (stop)
Transcribe to mRNA

DNA:
TAC TAC GGT AGG TAT A

*mRNA*:
AUG AUG CCA UCC AUA U
Change in 3rd Base May Not
Result in Error

Why not?


Amino acids have more than one codon
Example: proline


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Codons CCU, CCC, CCA, and CCG
CC - always codes for proline
Third base/nucleotide does not matter