Transcript Biotechnology lentikats in food industry

Lentikats Biotechnology
in Food Industry
Content
 Our aim
 What is Lentikats Biocatalyst
 Production of Lentikats Biocatalyst
 Benefits of Lentikats Biotechnology
 Lentikats Biocatalyst in Food Industry
 Our guarantees
 Our partners
 Contacts
LentiKat’s a.s. in Food Industry
 LentiKat’s a.s. follows up on nine year of research and development of Lentikats
Biotechnology.
 Our production know-how for commercial use of our Biocatalyst and for its industrial
applications is patented internationally.
 Our main focus is in intensification of production processes that leads to:
• higher process yields
• enhanced process stability,
• Increased revenue,
• Reduction of environmental footprint.
Biotechnologies are changing
the world…
Lentikats Biotechnology
Unique shape of the matrix and original immobilisation procedure enables to
encapsulate free enzymes or microorganisms (bacteria, yeasts, fungi) inside a
hydrogel porous carrier from polyvinyl alcohol (PVA). The advantages of PVA
matrix are:
• excellent physical and mechanical characteristics that result in a long-term
mechanical stability
• hardly biodegradable
• no toxic effects
Lentikats Biocatalysts enable easy separation of enzymes or microorganisms from
the bulk media and therefore can be used repeatedly.
With biotechnologies to
a better future…
Microencapsulation
vs.
Lentikats
Biotechnology
traditional proven technology
new method based on well-proven principle
spherical shape – limited diffusion to the
“core”
lentil-like shape – no diffusion limitations
„micro“ size
„mili“ size – easier separation
How does the Lentikats Biocatalyst work?
A maximum surface in combination with the minimum volume of Lentikats
Biocatalyst results in a substantial reduction of diffusion limitations
associated with the transfer of substrate into the matrix and the product
out of the matrix.
Why polyvinylalcohol?
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It is an inexpensive material
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It is a non-toxic material
Encapsulation takes place under optimal, cell-sparing conditions
It allows a production of hydrogel with excellent mechanical properties
It does not have any side effects on fermentation
It offers high capacity for the cell loading
The process is stable and easy to control
Biotechnologies are changing
the world…
How is Lentikats Biocatalyst produced?
Lentikats Biocatalysts
• Biocatalyst Types for Use in Lentikats Encapsulation:
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Free Cells
• dead cells
• viable, non growing cells
• viable and growing cells
Free or Immobilised Enzymes.
Lentikats Biotechnology allows to immobilise any type of
enzyme
• The size of Lentikats Biocatalyst pores varies depending on the size
and diameter of the Biocatalyst due to:
• PVA additive type (size of macromolecule)
• additive concentration
• biomass concentration
Lentikats Biocatalysts
Manufacturing Plant
Laboratory Units for Encapsulation
• For low-capacity production of encapsulates (especially for research
purposes), a laboratory device is available
What are the benefits?
Matrix containing high
concentration of
biomass
Higher yields (in comparison to
conventional technologies using
free biomass)
Possibility of repeated
usage
Operational costs
savings
Enhanced resistance of
modified and
immobilised biomass
Higher process
stability
Easy separation of
biomass from reaction
media
Reduced costs for
downstream processes
(separation)
Lower production of
waste and by-products
Environmentally
friendly disposal
Higher
reaction
rates
Reduction of
reaction tanks’
volume
Operational costs
savings (electricity,
heat)
..and also…
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Shortening of the reaction times
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Much higher yields in comparison to conventional technologies (up to 5
– 15 % higher)
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Repetitive use of the Biocatalyst, easy separation from media (sieve
separator)
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Higher productivity of the Biocatalyst
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Lower risk of contamination during the production process
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High stability and easy control of production process
Biotechnologies are changing the world…
Technological set-up
 - inlet of reaction media
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 - medium with Lentikats Biocatalyst
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 - filling of Lentikats Biocatalyst
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 - stirrer
 - sieve separator
 - outlet of separated media

 - outlet of Lentikats Biocatalyst
Lentikats and food industry
We currently offer solutions for the following processes:
 production of lactose free milk
Lentikats Biocatalyst with β-galaktosidase activity
 production of invert sugar
Lentikats Biocatalyst with invertase activity
 whey processing
Lentikats Biocatalyst with β-galaktosidase activity for:
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production of D-galaktose from whey lactose (pharmaceutical quality)
production of galacto-oligosaccharides from whey lactose
 production of special syrups
Lentikats Biocatalyst with invertase activity for production of glucose-fructose syrups
Lentikats Biocatalyst with immobilised glucoamylase for production of glucose from sugar
and starch-based materials or glucose syrups from maltotriose or maltose syrup
Lentikats and food industry
Following solutions are currently under development and testing:
 production of organic acids
Lentikats Biocatalyst with immobilised bacteria Bacillus coagulans for production of lactic
acid from lactose, glucose and sucrose
Lentikats Biocatalyst with immobilised filamentous fungi Aspergillus niger for production
of gluconic acid from sucrose and glucose
Lentikats Biocatalyst with immobilised filamentous fungi Aspergillus terreus for
production of itaconic acid from sucrose and glucose
Biotechnologies are changing the world…
Lentikats Biotechnology - examples
LENTIKATS BIOTECHNOLOGY FOR PRODUCTION
OF INVERT SUGAR:

enzyme: immobilised saccharase, 3-D-fruktofuranosidase for
hydrolysis of sucrose into glucose and fructose.
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optimum temperature 30–35°C
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optimum pH 4,5–5,0
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conversion rate 100 %
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operational stability: 600-1000 repeated batch conversions

application - processing of molasses or sucrose solutions
source
activity
Baker’s yeast
600-1000 g sucrose/ kg LB/hr
Lentikats Biotechnology - examples
LENTIKATS BIOTECHNOLOGY FOR PRODUCTION OF LACTOSE
FREE MILK
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enzyme: immobilised laktase, β-D-galactosid galactohydrolase for lactose hydrolysis.
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optimum temperature 30–50°C
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optimum pH 6,0–6,5
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conversion rate > 90%
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operational stability: 120–160 hours
source
activity
Kluyveromycer lactis
100-300 g GLC/ kg LB/hr
Investment and Investment Return
Guarantees
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You can easily verify our great results by testing our Biocatalyst in your or other
facilities with the material of your choice.
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Sample of Lentikats Biocatalyst free of charge
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Our company provides assistance and full technical support.
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Our company offers a long-term cooperation to companies, whose main concerns
are reduced investment costs and maximal process profitability.
Biotechnologies change the world…
Investment and Investment Return
Guarantees
We guarantee the following:

output parameters

operational
productivity
stability of Lentikats Biocatalyst and its
activity and
Our partners in the field of industrial
applications
Fermenta Biotech, DIL Complex, Ghodbunder
www.fermentabiotech.com
Road, Thane (West) - 400 610, Maharashtra, India
Protean, Pod Lesem 9, 373 16 Dobra Voda u Ceskych
Budejovic, Czech Republic
Pharmix s.r.o., Na Sadkach 2798, 767 01 Kromeriz,
Czech Republic
www.protean.cz
www.pharmix.cz
Our partners in the field of research and
development
Fermenta Biotech, DIL Complex, Ghodbunder Road,
Thane (West) - 400 610, Maharashtra, India
www.fermentabiotech.com
Institute of Microbiology,Academy of Science of the
Czech Republic
Vídeňská 1083, 142 20 Prague 4, Krč, Czech Republic
www.biomed.cas.cz
STU Bratislava, Fakulta chemické a potravinářské
technologie, Ústav biotechnologie a potravinářství,
Radlinského 9, 81237 Bratislava, Slovakia
www.stuba.sk
VŠCHT Praha, Ústav kvasné chemie a bioinženýrství,
Technická 5, 166 28 Praguea 6
www.vscht.cz
University of Alberta, Edmonton Alberta, T6G 2J9
Canada
www.ualberta.ca
Masaryk University, National Centre for Biomolecular
Research, Kamenice 5, 625 00 Brno-Bohunice
www.ncbr.chemi.muni.cz
Thank you for attention
Company Headquarters:
LentiKat´s a.s.
Evropska 423/178
160 00 Prague 6
Czech republic
Tel.:
Fax:
E-mail:
+ 420 224 362 461
+ 420 224 362 463
[email protected]
Production plant and laboratories
LentiKat´s a.s.
Pod Vinicí 83
471 27 Stráž pod Ralskem
Czech republic
Tel.:
Fax:
E-mail:
+ 420 487 851 393
+ 420 487 851 399
[email protected]