Transcript Biotechnology lentikats in food industry

Lentikats Biotechnology
in Pharmaceutics
Content
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Our focus
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Protein engineering
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What is Lentikats Biocatalyst
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Production of Lentikats Biocatalyst
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Benefits of Lentikats Biotechnology
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Lentikats Biocatalyst in Pharmaceutics
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Our guarantees
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Our know-how, references and experience
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Our partners
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Contacts
LentiKat’s a.s. in Pharmaceutics
• Our main focus in the pharmaceutics is on production
of robust biocatalysts by application of the following
scheme:
1) molecular cloning and production of recombinant proteins,
2) proteins’ activity and stability enhancement by application of
protein engineering methods,
3) immobilisation using Lentikats Biotechnology
• Our approach leads to:
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higher process yields,
enhanced process stability,
improved process economics.
Protein engineering
We offer molecular design and production of any enzyme
in a recombinant host system.
Using state-of-the-art protein engineering technology we gain:
• Higher enzymatic activity
• Improved robustness of the enzyme
Our partners in the field:
• National Centre For Biomolecular Research, Masaryk University
• Protean s.r.o.
Biotechnologies are changing
the world…
Lentikats Biotechnology
Unique shape of the matrix and original immobilisation procedure enables to
encapsulate free enzymes or microorganisms (bacteria, yeasts, fungi) inside a
hydrogel porous carrier from polyvinyl alcohol (PVA). The advantages of PVA
matrix are:
• excellent physical and mechanical characteristics that result in a
long-term mechanical stability,
• hardly biodegradable,
• no toxic effects.
Lentikats Biocatalysts enable easy separation of enzymes or microorganisms from the bulk media
and therefore can be used repeatedly.
With biotechnologies to
a better future…
Microencapsulation
vs.
Lentikats
Biotechnology
traditional proven technology
new method based on well-proven principle
spherical shape – limited diffusion to the
“core”
lentil-like shape – no diffusion limitations
„micro“ size
„mili“ size – easier separation
How does the Lentikats Biocatalyst work?
A maximum surface in combination with the minimum volume of Lentikats
Biocatalyst results in a substantial reduction of diffusion limitations associated
with the transfer of substrate into the matrix and the product out of the
matrix.
Why polyvinylalkohol?
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It is an inexpensive material
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It is a non-toxic material
Encapsulation takes place under optimal, cell-sparing conditions
It allows a production of hydrogel with excellent mechanical properties
It does not have any side effects on fermentation
It offers high capacity for the cell loading
The process is stable and easy to control
Biotechnologies are changing
the world…
How is the Lentikats Biocatalyst produced?
Lentikats Biocatalysts
• Biocatalyst Types for Use in Lentikats Encapsulation:
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Free Cells
• dead cells
• viable, non growing cells
• viable and growing cells.
Free or Immobilised Enzymes.
Lentikats Biotechnology allows to immobilise any
type of enzyme
• The size of Lentikats Biocatalyst pores varies depending on
the size and diameter of the Biocatalyst due to:
• PVA additive type (size of macromolecule)
• additive concentration
• biomass concentration.
Lentikats Biocatalysts
Manufacturing Plant
Laboratory Units for Encapsulation
• For low-capacity production of encapsulates
(especially
for research purposes), a laboratory device is available.
What are the benefits?
Matrix containing high
concentration of
biomass
Higher yields (in comparison to
conventional technologies using
free biomass)
Possibility of repeated
usage
Operational costs
savings
Enhanced resistance of
modified and
immobilised enzyme
Higher protein
resistance and activity
durability
Immobilisation
prevents enzyme
aggregates disruption
Reduced costs for
downstream processes
(separation)
Lower production of
waste and by-products
Environmentally
friendly disposal
Higher
reaction
rates
Reduction of
reaction tanks’
volume
Operational costs
savings (electricity,
heat)
..and also…
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Shortening of the reaction times
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Much higher yields in comparison to conventional technologies
(up to 5 – 15 % higher)
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Repetitive use of the Biocatalyst, easy separation from media
(sieve separator)
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Higher productivity of the Biocatalyst
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Lower risk of contamination during the production process
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High stability and easy control of production process
Biotechnologies are changing the world…
Technological set-up
 - inlet of reaction media
 - filling of Lentikats Biocatalyst
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 - stirrer
 - sieve separator
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 - medium with Lentikats Biocatalyst
 - outlet of separated media
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 - outlet of Lentikats Biocatalyst
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Lentikats Solutions
Lentikats Biotechnology is used in the following pharmaceutical
processes:
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6-aminopenicillanic acid production (6-APK)
(immobilized enzyme with activity of penicillin G-acylase)
7-aminodeacetoxycephalosporanic acid production (7-ADCK)
(immobilized enzyme with penicillin G-acylase activity)
Following products are available for chemical industry:
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production of D-galactose from lactose
(immobilised enzyme β-galactosidase, p.a.)
production of galacto-oligosaccharides from lactose
(immobilised enzyme β-galactosidase)
Lentikats Solutions
Products that are currently under development and being
tested
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Amoxicillin synthesis (synthesis of amoxicillin from phydroxyphenylglycine methyl ester and 6-aminopenicillanic acid by
immobilized penicillin G acylase)
production of lactic acid from glucose, sucrose and lactose
(immobilised bacteria Lactobacillus coagulans)
production of 1,3 – propanediol
(immobilised bacteria Clostridium butyricum)
production of dihydroxyaceton
(immobilised bacteria Gluconobacter oxydans)
Lentikats Solutions
for Antibiotics Production
LENTIKATS BIOTECHNOLOGY FOR PRODUCTION OF ANTIBIOTICS
Enzyme:
immobilised penicillin G-acylase for production of antibiotics.
Specific Activity: specific activity is determined by use of standard solution and conditions in
batch
test:
- pen G: 8% w/v
- temperature: 28°C
source
activity
- pH: 8.0 (5 mM phosphate buffer)
E.coli
70 U / g LB wet weight
- conversion time: 80-120 min
380 U / g LB dry weight
- conversion rate: 98-99%
Operation Stability: 600-800 repeated batch conversions.
Investment and Investment
Return Guarantees
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You can easily verify our great results by testing our Biocatalyst in your
or other facilities with the material of your choice.
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Sample of Lentikats Biocatalyst free of charge.
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Our company provides assistance and full technical support.
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Our company offers a long-term cooperation to companies, whose main
concerns are reduced investment costs and maximal process profitability.
Biotechnologies change the world…
Investment and Investment
Return Guarantees
We guarantee the following:
1.
delivery of production facilities and devices within
24 months
2.
output parameters
3.
operational stability of Lentikats Biocatalyst and
its activity and productivity
Our know-how, references and
experience in pharmaceutics
• Production and supply of Lentikats Biocatalyst with Penicillin
G-acylase activity for production of antibiotics
Our partners in the field
of industrial applications
Fermenta Biotech, DIL Complex, Ghodbunder
www.fermentabiotech.com
Road, Thane (West) - 400 610, Maharashtra, India
Protean, Pod Lesem 9, 373 16 Dobra Voda u Ceskych
Budejovic, Czech Republic
Pharmix s.r.o., Na Sadkach 2798, 767 01 Kromeriz,
Czech Republic
www.protean.cz
www.pharmix.cz
Our partners in the field of research
and development
Fermenta Biotech, DIL Complex, Ghodbunder Road,
Thane (West) - 400 610, Maharashtra, India
www.fermentabiotech.com
Institute of Microbiology,Academy of Science of the
Czech Republic
Vídeňská 1083, 142 20 Prague 4, Krč, Czech Republic
www.biomed.cas.cz
STU Bratislava, Fakulta chemické a potravinářské
technologie, Ústav biotechnologie a potravinářství,
Radlinského 9, 81237 Bratislava, Slovakia
www.stuba.sk
VŠCHT Praha, Ústav kvasné chemie a bioinženýrství,
Technická 5, 166 28 Praguea 6
www.vscht.cz
University of Alberta, Edmonton Alberta, T6G 2J9
Canada
www.ualberta.ca
Masaryk University, National Centre for Biomolecular
Research, Kamenice 5, 625 00 Brno-Bohunice
www.ncbr.chemi.muni.cz
Contacts
Company Headquarters:
LentiKat´s a.s.
Evropská 423/178
160 00 Praha 6
Czech republic
Tel.:
Fax:
E-mail:
+ 420 224 362 461
+ 420 224 362 463
[email protected]
Production plant and laboratories
LentiKat´s a.s.
Pod Vinicí 83
471 27 Stráž pod Ralskem
Czech republic
Tel.:
Fax:
E-mail:
+ 420 487 851 393
+ 420 487 851 399
[email protected]