Transcript Acetylcholine Receptor Binding Toxins

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Acetylcholine Receptor Binding Toxins
Naja naja:
α-cobra toxin source
Hypothesis:
Similar functioning proteins that bind the ACh
receptors will have similar amino acid sequences.
Bungarus multicinctus:
κ-bungarotoxin toxin source
Results:
*Overlay: α-cobra toxin a (Y), κ-bungarotoxin (B),
Cobratoxin (G), Erabutoxin (O)
ACh Receptor
*Phylogenetic Tree:
Conclusion:
Four (4) toxins that affect ACh
receptors were found to be
closely related evolutionarily
resulting in proteins that have
structural areas of similarity in
their amino acid sequences.
TLECHNQQSSQTPTTTGCSGGETNCYKKRWRD----HRGYRTERGCG--CPSVKN-GIEINCCTTDRCNN-*Associated Amino Acid Residue Sequences:
-RICFNHQSSQPQTTKTCSPGESSCYNKQWSD----FRGTIIERGCG--CPTVKP-GIKLSCCESEVCNN-α-bungarotoxin
TIVCHTTATS-PISAVTCPPGENLCYRKMWCDAFCSSRGKVVELGCAATCPSKKP-YEEVTCCSTDKCNPHP
κ-bungarotoxin
TRTCLISPSS---TPQTCPNGQDICFLKAQCDKFCSIRGPVIEQGCVATCPQFRSNYRSLLCCTTDNCNH-Cobratoxin
Erabutoxin
TLECHNQQSSQTPTTTGCSGGETNCYKKRWRD----HRGYRTERGCG--CPSVKN-GIEINCCTTDRCNN--RICFNHQSSQPQTTKTCSPGESSCYNKQWSD----FRGTIIERGCG--CPTVKP-GIKLSCCESEVCNN-TIVCHTTATS-PISAVTCPPGENLCYRKMWCDAFCSSRGKVVELGCAATCPSKKP-YEEVTCCSTDKCNPHP