Transcript Andrew & Muhammad

Effects of Glucose on the Growth
Curve of Pseudomonas fluorescens
Muhammad Khan & Andrew Passer
November 28, 2011
Our Question:
What effect does the addition of glucose have
on the growth curve of Pseudomonas
fluorescens?
Interest in Glucose
Experiment 2
Experiment 3
?
?
Experiment 4 Supports Experiment 3
Is glucose metabolism in P. fluorescens
acquired or inherited?
We could not “shock” P. fluorescens into increased growth by raising it in a
glucose-rich, tryptone-poor environment prior to inoculation.
Calculating "Growth Points"
What Concentration of Glucose Gives
the Highest Level of Growth?
Average: 3.418% glucose
Conclusion
• Addition of glucose has a positive effect on
the growth of P. fluorescens.
• Glucose metabolism is an inherited trait for
this species.
• Optimum growth occurs at about 3.4%
glucose.
Discussion & Future Directions
• More time needed for P. fluorescens to grow.
• Is the prolonged increase in OD actual
growth?
• What are the waste products of the different
metabolic pathways (amino acid vs. sugar)?
• How do the waste products affect the
stationary phase?
Any questions?