Transcript 3.Presentation Template Dr.Diya Alsafadi3x

Utilizing Science & Technology and Innovation
for Development
Study the interaction of
nanoparticles with proteins
Marriott Hotel- Amman, August 13th, 2015
Project Team
•
Dr. Mayyas Al-Remawi
Taif University/KSA
•
Dr. Diya Alsafadi
Royal Scientific Society/Jordan
Brief Description
The development in the nanotechnology in the last decade has
dramatically widened the prospect of using nanoparticles (NPs)
with unique properties.
Brief Description
- Due to size, nanoparticles more easily taken up
by the human body and can cross biological
membranes, cells, tissues and organs more
efficiently than larger particles.
- Less than 100nm enter cell, less than 40nm enter
nucleus, less than 35nm pass Blood Brain
Barrier.
Problem
 Disposal and environmental fate?
 Potential toxicity & Interactions with living
cells
Nanoparticle-Protein Corona
 Nano- particles in
physiological fluids
interact initially with
the proteins.
 Formation of the
corona is a dynamic,
competitive process
Justifications
100 nm
Chitosan Nanoparticles
Used as drug delivery
Silver Nanoparticles
BSA & HSA Used in electronics, biosensing, clothing, paints,
cosmetics and medical devices
Objectives
<Overall objective> Evaluate the biological hazard
of nanoparticles by investigating its interaction
with proteins.
<Specific objective> Assess the effect of silver and
chitosan nanoparticles on serum proteins using
spectroscopic techniques.
Scope of work/Duration
Estimated Budget
Scope of work: Nanosafety and nanomedicine sciences.
Duration: 24 months
Estimated Budget : 210,000 JD
Methodology of Implementation
Nanoparticle synthesis
In vivo effects
Dispersion and
characterisation
Protein Corona
Functional impacts /
toxicity
Expected output
By understanding the bionanointeractions, we will
be able to
•Deliver
medicine/ treatment to new sites
in the body and cure people.
•Avoid harming people through the
application of nanotechnology.
Impact
• The project will provide a significant contribution to
the implementation of community environmental
policy and legislation in the field of nanosafety and
nanotoxicity.
• The outcomes and recommendations of the project
will be sent to Ministry of Environment, Ministry of
Health and JFDA.
Sustainability
Institutional sustainability
 Build a life communications plan between the academy
and industry.
 Prepare and market short courses related to project out
comes in collaboration with public and private sectors.
Policy level sustainability
Through proper channels we will create communications
with the policy makers to approve legislations to encourage
public and industrial sector to be aware for the using of
NPs.
Action Plan
In vivo Functional
effects impacts /
toxicity
NP- Protein Corona
synthesis &characterization
NP-synthesis &
characterization
Q1
Q2
Q3
Q4
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Chitosan
NPs synthesis
SEM, TEM, Zeta Potential
Ag-NPs synthesis
XRD, AA and ICP
Chitosan NP-protein
corona
Ag-NP-protein
corona
UV.VIS,CD and FTIR
Fluorescence spectroscopy,
SDS-PAGE & Gel filtration
Functional impacts
/ toxicity
In vivo effects
Taif University
Royal Scientific Society
Thank You