Transcript AIM - Politecnico di Torino

http://www.composites.polito.it
Research activities on Biomaterials: staff
Permanent Staff
Post-doc
Monica Ferraris
Francesco Baino
Silvia Spriano
Cristina Balagna
Enrica Verné
Marta Miola
Chiara Vitale Brovarone
Sara Ferraris
Giorgia Novajra
PhD students
Research fellows
Matteo Bruno
Guanjun Pan
Xin Zhang
I..M. - Institute Meeting
Marta Gallo
Francesca Tallia
Metallic Biomaterials
(Titanium and
cobalt alloys, NiTi)
[email protected]
Bioactive glasses, coatings
and surface functionalization
Atoxic metal matrixes
for tools
[email protected]
BIOMATERIALS
and TOXICITY
[email protected]
Biomaterials for
magnetic induction hyperthermia
and drug targeting
[email protected]
http://www.composites.polito.it
bioresorbable glasses
for soft and hard
tissue regeneration
[email protected]
Macroporous scaffolds for
bone tissue engineering
and drug delivery
[email protected]
Antibacterial materials
and coatings
[email protected]
Bionica Tech is a spin-off company of Politecnico di Torino.
Born in January 2010.
The MISSION:
Through Bionica Tech patented
ideas acquire a high added value,
turning into processes/products
tailored to meet the specific
requirements of the global
biomedical market.
COMPLETE VALIDATION:
•chemical-physical tests
•mechanical tests
•in-vitro cell cultures
•in-vivo and clinical tests
•validation master plan for the product
certification, etc..)
•detailed business process protocol.
CEO: Davide Caregnato
ASSOCIATES:
Enrica Vernè
Chiara Vitale Brovarone
Silvia Spriano
Pier Vincenzo Pellegrino
Claudio Sguazzini
Andrea Francone
Franco Perona
Antonio Marino
COLLABORATORS:
Marta Miola (R&D orthopaedic bone
cements)
Lucia Pontiroli (R&D spine cements)
Sara Ferraris (R&D titanium alloys)
Patent: Verné E, Miola M, Ferraris S, Massé A, Bistolfi A, Crova M, Maina G,
Composite bone cements with a PMMA matrix, containing bioactive
antibacterial glasses or glass-ceramics.
SilVerre: BIOACTIVE AND ANTIBACTERIAL
COMPOSITE BONE CEMENT
Patent: Vitale-Brovarone C, Verné E, Bergui M, Onida B, Baino F, Miola M,
Ferraris S., Tallia F, Injectable osteoinductive bone cements.
Spine-Bond and Spine-Ghost: INNOVATIVE
BIOACTIVE VERTEBRAL CEMENTS
Patent: Spriano S, Verné E, Ferraris S, Multifunctional titanium surfaces
for bone integration.
MimeTi: BIOMIMETIC TITANIUM
Biomaterials for magnetic induction hyperthermia
E. Verné, M. Miola, S. Ferraris, M. Bruno
MIH uses magnetic materials which can generate heat under an
alternating magnetic field (usually by hysteresis loss and eddy current
loss) to destroy solid tumours.
Materials: magnetite-containing bioactive glass-ceramics as bulk, powders or
disperse phase into a PMMA bone cement.
Bioactivity: ability to chemically bond to the bone tissue (bone substitution
after solid tumours removal).
Biomaterials for Magnetic and Immunological Drug Targeting
E. Verné, M. Miola, S. Ferraris
AIM: preparation of smart nanoparticles with a central core of nanosized
magnetite crystals, embedded into a silica-based amorphous matrix able to
recognize cancer cells (functionalization with proper peptides) for diagnosis and
therapy (localized drug delivery).
MDT: magnetic particles (carriers) to deliver and release the
chemotherapeutic agent to the desired site (tumour).
IDT: inclusion of a targeting ligand (antibody) that binds to a specific
antigen and deliver the drug-loaded nanoparticle to the tumour cells, upon
specific recognition.
PROGETTO S. PAOLO 2011 “Nanovettori intelligenti per la diagnosi e la terapia in Oncologia”.
Antibacterial materials and coatings
M. Ferraris, E. Verné, S. Perero, M. Miola, S. Ferraris, C. Balagna, F. Baino
AIM: to obtain an effective and versatile antibacterial layer
 Stable (thermally, chemically … )
 Applicable to different surfaces
 With tailorable antibacterial activity
 Broad spectrum of activity
 Low resistance development
CO-SPUTTERED SILVER
NANOCLUSTERS-SILICA LAYER
SUBSTRATE
• Regione Piemonte, Italy, with ‘‘NABLA- Nanostructured Antibacterial Layers.’’
• EU Project-NASLA-FP7-SME-2010-1 (Project 262209)
Surface functionalization of silica based biomaterials, for
osteointegration and therapy:
Surface functionalization of bioactive glasses
E. Verné, S. Ferraris, X. Zhang
Natural therapeutic agents (resveratrol)
Drugs (in situ drug delivery)
Peptides/enzymes (biological signals)
Spacer (3-aminopropyltriethoxysilane)
Bioactive glass
Bioactive Surface (inorganic chemical signals)
Surface functionalization for biosensing:
Folic acid grafting to a Silver nanoclusters-silica thin film.
FA
Ag
Ag
FA
Ag
Ag
FA
Absorbance [a.u]
M. Ferraris, E. Verné, S. Perero, S. Ferraris
Characteristic SPR
absorption
New SPR
absorption
Ag
Silver nanoclusters-silica
composites thin films
Wavelength [nm]
Silver nanoclusters silica thin film posses a characteristic SPR absorption that can
be employed for sensing. Surface grafting of molecules able to specific bind to
characteristic markers (for example Folic Acid, which is over expressed by several
cancer cells)  The specific reaction can cause a readable shift in the SPR
absorption peak  detection.
BIOACTIVE BONE CEMENTS WITH ANTIBACTERIAL
PROPERTIES
E. Vernè, M. Miola
PROBLEM: DEVELOPMENT OF POST-SURGICAL INFECTIONS
SOLUTION: To develop an innovative PMMA-based bone cement
for orthopaedic prostheses fixation or for temporary prostheses
able to reduce the infections development and promote tissue
integration.
Ca
O
P
C
NaMg
I.F.M. - Institute Meeting
Surface treatments of Ti and Ti alloys
(S.Spriano-S.Ferraris):
1. Nano-textured and hydroxylated surfaces presenting high
osteointegration ability (patented by Polito-licensed to Bionica
Tech: MimeTi)
2. Functionalized Ti surfaces (patented by Polito)
Aim: chemical and thermal treatments in order to induce an
inorganic and biological bioactivity on Ti (apatite precipitation-cell
adhesion and differentiation)=> rapid bone healing
Applications: dental and orthopaedic implants
3. Anti-bacterial behaviour of modified Ti surfaces
(patented by Polito)
Aim:
Nanoparticles
of
antibacterial metals are tightly
incorporated
in
the
nanostructured oxide layer
allowing a gradual release of
the antibacterial agent into the
physiological media..
Applications: dental and
orthopaedic implants
NEAR NET-SHAPE CASTING OF SHORT NITINOL
TUBES FOR BIOMEDICAL APPLICATIONS
TINET Regional Project
(S.Spriano-C.Balagna-G.Pan)
Aim: The production of short Nitinol tubes responding to the
requirements of the cardiovascular field (High homogeneity and
reproducibility of the thermal and mechanical properties, low
content of precipitates and inclusions, super-elastic behaviour at
body temperature)
Applications: tubes for vascular stents
TANTALUM COATING ON Co-Cr BIOMEDICAL ALLOYS
Patented by Polito
(S.Spriano-C.Balagna)
Ta HCP
Ta2C
TaC
CoCrMo
0.1 mm3
Ta-based coating
CoCrMo
substrate
0.2 mm3
Aim: A surface heat treatment in molten salts in order to get a
higher wear resistance and biocompatibility on Co based alloys
(formation of Ta carbides)
Applications: hip and knee arthroprosthesis
3D- scaffolds for bone substitution
(C. Vitale-Brovarone, F. Baino)
AIMS:
-Bioactivity (ability to chemical bond with the surrounding bone tissue and to
stimulate bone regeneration)
-Structure with open and interconnected pores (analogous to spongious bone)
-Mechanical strength (2-12 MPa)
-Bioresorbability (temporary devices)…phosphate glasses
I.F.M. - Institute Meeting
Osteoblast colonisation and
calcium nodule deposition
MATCh “Mononlock Acetabular cup with Trabecular-like
Coating” FP7-SME-2011 C. Vitale-Brovarone
AIM: To realize a monoblock acetabular cup
able to osteointegrate with the pelvis bone
APPLICATION: Orthopaedics
Analogous concept can be applied for
knee prosthesis
PATENT: WO 2008/146322 A2
17
BIOACTIVE GLASS/POLYMER COMPOSITE SCAFFOLDS
FOR OSTEOCHONDRAL TISSUE ENGINEERING
(C. Vitale Brovarone, F. Baino, G. Ciardelli)
AIM: Regeneration at the interface of bone/cartilage
DIMEAS
DISAT
Bioactive glass (CEL2)
Polymer (chitosan + gelatin)
Freeze-drying
Composite scaffold
Periostium derived precursor
cells
18
BIORESORBABLE PHOSPHATE GLASSES
(C. Vitale Brovarone, G. Novajra, F. Baino)
AIM: To obtain temporary materials able to guide the physiological regeneration
of the tissue
APPLICATION: bone substitution
Bioerosion in contact with physiological fluids
with kinetics that can vary from days up to years.
Gradual enhance of the porosity during
resorption
Resorbable glass fibers for nerve regeneration
(C. Vitale Brovarone, G. Novajra, D. Milanese, J.
Lousteau)
AIM: To guide the axon growth
APPLICATION: regeneration of pheripheral nerve and
growth factor/drug delivery
Hollow fibres/PCL tube
Hollow fibers for
biomolecules
delivery
Collaboration with DIMEAS
(Ciardelli)
Collaboration with UNITO
(Fornaro, Geuna, Raimondi)
Mesoporous glasses
(C. Vitale Brovarone, L. Pontiroli F. Baino, B. Onida)
High surface area (300 m2/g, nanopores of controlled
size and geometry (2-50 nm)
AIMS: Drug delivery system (in combination with glassceramic scaffolds or as disperse phase in a polymeric
matrix)
APPLICATIONS: bone substitution, muscle and liver
regeneration…
AIMS: injectable cement for spinal surgery
(resorbable or not)
APPLICATION: vertebroplasty
WO2011141889
(A1)
cement patent by POLITO
Injectable
bone