Transcript Synthetic Organelles

Physical insulation of
pathways or functions
How to build complex
synthetic circuits?
• Electronic hard wired: reusability of
components.
• Biology is diffusive: crosstalk
We need to find a way to insulate the
different components circuits.
Pathway insulation methods
Orthogonality (chemical insulation)
Family of orthogonal parts
Physical insulation
Scaffolding
Synthetic organelles
Synthetic ecosystems
PROJECT 2:
BUILDING A NEW ORGANELLE
FOR SYNTHETIC BIOLOGY
A quick recap
wild-type cell
create “synthesome”
use “synthesome”
STEP 1: Create spatially
distinct membrane
compartment that has
unique molecular identity
STEP 2: Recruit proteins to
carryout any synbio
process of choice (e.g.
drug or biofuel factory)
An external organelle
External organelles
producing cell
Targetting
Components of
a pathway
to secretion
External organelles
producing cell
An external organelle
External
organelle
Chassi
s
(Feeder)
An external organelle
Attachment
transmembrane
proteins
Chassi
s
(Feeder)
External organelle
Chassi
s
(Feeder)
External organelle
Chassi
s
(Feeder)
communication
External organelle composition
(1)
Function?
Receiver
factory
Chassis
(Feeder)
Chassi
s
(Feeder)
sender
Advantage: part reusability
Example: multicolor Hasty oscillator
State output
Chassis
(Feeder)
Chassi
s
(Feeder)
output
External organelle composition
(2)
pathway
product
Starting
product
Chassi
s
(Feeder)
Natural example: Nanoarchaeum
equitans
QuickTime™ and a
BMP decompressor
are needed to see this picture.
Nanoarchaeum: ABC
transporters
Chassi
s
(Feeder)
Communication:
Within eukaryotes, ABC-transporters mainly
transport molecules to the outside of the plasma
membrane or into membrane-bound organelles
such as the endoplasmic reticulum,
Natural examples
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Gap junctions
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Bacterial conjugation