Transcript Tissue Bioreactor

Tissue Bioreactor
by
Karen Chen, Rachel Mosher, Dustin Gardner, Richard Bamberg
Client: Susan Thibeault
Advisor: Brenda Ogle
(March 9, 2007)
Outline
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Problem Statement
Background
Summary PDS
Design Alternatives and Matrix
Future Work
Conclusion
Problem Statement
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Improve Current Design:
 No contact between vibrating strips
 Inconsistent distribution of vibration
 Tecoflex is a porous substrate
“More accurately simulate human vocal folds”
Research: growth of vocal fold fibroblasts
Background – Vocal Folds
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2 mucous membranes
Extracellular matrix
Cartilage
Horizontally stretched
across larynx
Vibration ranging 0400Hz
Modulate airflow from
lungs during phonation
Background – Vocal folds
Background - Bioreactor
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Supports/mimics a
biological system
Similar to vocal fold
environment
Cell culturing/seeded
Tecoflex strips
Vibration
Closed system
Fluid provides nutrition
Client Requirements
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Concerted bar vibration (0-400Hz frequency)
Contact between strips during vibration
Cartilaginous structures around strips
Easy to sterilize with disposable parts
Tecoflex substrate alternative
Target Cost $5,000
Bioreactor Specifications
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Culture vocal fold fibroblasts for research
Research fibroblast reactions to stimuli (vibrations)
Environment monitored by software interface
Manual replacement and sterilization of components
Size: Slightly larger than T-75 flask.
Weight: Function of bioreactor unaffected
Operating Environment and
Considerations
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Effective simulation of vocal fold environment
Uniform vibrations to cell-seeded strips
Temperature range: Incubator (37°C)
Pressure: Negligible, ΔP = ½psi
Corrosion: Fluids retained in T-flask only
Bioreactor Lifespan
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Long shelf life
Vibration motor (actuator) is life-limiting
T-flask properly sealed and disposable
T-flask disposed after use (several weeks)
Periodic cleaning of mechanical components
Minor sterilization of some components
Project Objectives
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Improve previous design for research
Only one prototype will be constructed
Simultaneous use of multiple bioreactors
Research purposes (several dozen nationwide)
Original bioreactor was $15,000
No intent to patent design
Design Alternatives
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Double-sided vibration, angled strips
Double-sided vibration, parallel strips
Single-sided vibration, angled strips
Single-sided vibration, parallel strips
Single-Sided Vibration
Pros
 Easier to fabricate
 Less expensive
Cons
 May not provide even
vibratory distribution
Parallel Strips
Pros
 Strips make contact
across entire length
 Easier to implement than
angled strips
Cons
 Adjustable angled strips
more realistic
Design Matrix
Cost
Effectiveness (110)
Frequency
Distribution
(1-30)
Realistic
Imitation of
Environment
(1-20)
Feasibility /
Practicality of
Design
(1-30)
Client
Contentedness (110)
Total
(100)
Two-sided
Vibration
V-shape Strips
5
21
18
12
10
66
Two-sided
Vibration
Parallel Strips
7
23
16
16
7
69
One-sided
Vibration
V-shape Strips
7
18
14
18
7
64
10
18
14
24
7
73
One-sided
Vibration
Parallel Strips
Future Work
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Order custom parts
Fabricate parts ourselves
Put prototype together
Testing and Analysis
Conclusion
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Bioreactor has many complicated components
Research vocal folds and drug therapy
Proper vibratory stimulus essential
Single-sided vibration of parallel strips
Parts need ordering and fabrication
Credits