Transcript Biodegradable Polymer Nanoparticles for drug delivery through

Nanotech in Biomedicine:
Biodegradable Polymer Nanoparticles
for drug delivery
through mucosal Membranes
By Group #3
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Phillip Keller
Micheal Jones
Krista Melish
James Kancewick
Overview
• Background and Introduction
– Nanotech in Biomedicine
• Problem and Basic Principles
– Nano Particle Immobility/Transport Process
• Experiment and Results
– Polymer Prep/Transport Characteristics
• Improvements and Future Research
Introduction
• Nanotechnology
– Very Small
– Very Powerful
– Many Applications
– Rapid Research
Growth
http://www.redicecreations.com/ul_img/2353nanotechnology.jpg
Introduction: Biomedicine
• Biomedicine
– Drug delivery
– Biodiagnostics
– Biosensors
– Bioimaging
http://www.tlc2.uh.edu/Research/Institutes_Cent
ers/visual_logo.jpg
Drug Delivery
• Selective Targeting
– Organs
– Pathogens
– Cancer Cells
• Protects Deliverable
– Less Medicine Wasted
• Controllable Release
http://web.mit.edu/newsoffice/2008/nanoparticles.jpg
Biodiagnostic
• Detection of Nucleic Acids
– PCR (Polymerase chain reaction)
• Detection of Proteins
– High levels of certain proteins
are indicative of various
cancers and diseases
http://www.npl.co.uk/upload/img_200/
AB_Still_z_0022_prv.jpg
Biosensors
http://life.nctu.edu.tw/~cslin/Tw/Research/Biosensing%20System.files/image003.gif
Bioimaging
• Enhance Current Imaging Techniques
– Compliment MRI/CT
– Provide contrast in previously unobtainable areas
– Reduce need for exploratory surgery
http://www.feilab.org/pictures/facility/Flurosecence_Results.jpg
Problems with Nano Particle Drug Delivery
100X Salival Mucus
http://www.tryingtoconceive.com/store/fern1.jpg
Mucus Penetration
Cervicovaginal Mucus Fibers
• Cystic Fibrosis
– Thickening of lung
mucosal layers
• Eye
• Gut
– Drug delivery to impede
cancer growth
• Female Reproductive
Diseases
http://www.sciencedirect.com/science?_ob=ArticleURL
Mucus Penetration
http://www.sciencedirect.com/science?_ob=MiamiCaptionURL&_method=retrieve&_udi=B6T3R-4V4KPJ9-3&_image=fig6&_ba=6&_user
Particle Biodegradability and Drug Delivery
• No Interaction with Mucus Fibers
• Dense PEGylation of particle surface allows mucus
penetration due to neutral charge distribution
http://www.sciencedirect.com/science?_ob=MiamiCaptionURL&_method=retrieve&_udi=B6T3R4V4KPJ93&_image=fig8&_ba=8&_user=952835&_coverDate=02%2F27%2F2009&_rdoc=1&_fmt=full&_orig=search&_cdi=4953&_issn=0169409X&_pii=S016
9409X08002652&view=c&_acct=C000049198&_version=1&_urlVersion=0&_userid=952835&md5=be4fd7ee848e89aca12a6089c26869d2
Biodegradable Nanoparticle
With Encapsulated Drug
Synthesis of PSA-PEG
•One Step Formulation Process
•Diblock Copolymers
•Covalently Bonded
Mucus-Penetrating Nanoparticle
• Dense Surface Coating
of PEG
• Low Molecular Weight
• Neutral Surface Charge
– Non-Penetrating particles
have highly negative
surface charge
Flexible Drug Delivery
•Controlled Drug Release
•Vary ratio of PEG to PSA to alter particle
degradation rate
Physicochemical Properties of Nanoparticles
• Zai Potential
• Surface charge characteristic
• Dw/Dm
• Ratio of dispersion coefficients for particle in water
compared to human cervical-vaginal mucus
Results & Conclusions
•Rapid Transport
•Can Penetrate CVM Rapidly
Penetration in Cystic Fibrosis Sputum
•Rapid Penetration of CF Sputum
Improvements and Further Research
• Increase Particle Transfer
Rates
– Adapt to more specific
tissues
http://www.intel.com/technology/itj/2008/v12i3/3-paper/figures/Figure6.gif
Improvements and Further Research
• Develop Applications for a Wider
Range of Diseases
– Create nanoparticles capable of
accepting a wider array of drugs
• Develop more chemically gentle
manufacturing processes for coating
particles in PEG
http://www.labgrab.com/files/pictures/users/blogs/HIV.jpg
Improvements and Further Research
• Reduce Cost of
Production
– Make economically
viable
• Increased targeting
reduces dosage and
drug waste
http://www.maserati-rc.org/PicLib/Factory_new_production_line_3.jpg
Questions?
Resources
• Tang, Benjamin, Michelle Dawson, Samuel Lai, Ying-Ying
Wang, Jung Suk, and Ming Yang. "Biodegradable polymer
nanoparticles that rapidly penetrate the human mucus barrier."
Proceedings of the National Academy of Sciences 106.46
(2009): 19268-9273.
• Various websites listed in slides
Rebuttal From Group U3
Micheal Jones
Phillip Keller
Krista Melish
James Kancewick
media.podcasts.ox.ac.uk/mat/nanotechnology/nanotechn
ology_albumcover.jpg
What we did well
– Confidence
– Graphics
– Well Dressed
– Basic Intro
– More in depth article
http://www.ipcom.co.nz/tl_files/images/DiverBusinessman-web.jpg
Areas For Improvement
• Elimination of filler words
• Increasing presentation time
and content
• Better eye contact with the
audience
• Relabeling graphics with small
words
• Increase group understanding
of the article
http://www.ipcom.co.nz/tl_files/images/
Diver-Businessman-web.jpg
Biodegradable Polymer Nanoparticles for drug delivery
through mucosal Membranes
Group U1
• The overall presentation was very good
and interactive with the audience.
•The nanoparticle PSA-PEG showed to be
a very effective polymer since it has good
diffusion through some specific organic
membranes
•Further research can be done on
improving the efficiency in delivering drugs
• Its still a problem to power this particles.
http://science-hub.com/2009/06/platinum-particles-inthe-form-of-japanese-sweets/
•We still have to do work on targeting the
right receptors because in body we may
have several similar mucosal membranes
with similar biological and chemical
properties that are healthy.
Review of Group U3’s Presentation-
By Group U2:
-Kyle Demel
-Keaton Hamm
-Bryan Holekamp
-Rachael Houk
http://dipc.ehu.es/nano2006/Nanoparticles.jpg
http://radio-weblogs.com/0105910/
images/nanoparticles.jpg
The presenters could have improved in the following areas:
•
Presentation too short:
– 30-minute presentation only lasted 15
– Needs elaboration on further research:
• Delivery with viral protein coatings
• Transfer through cellular membranes
– Explain the pictures on the slides; do not
just add pretty pictures because you
have to
http://www.ediblecomputerchips.com/Nano-Tech/Final/Seven-A.jpg
•
Oral delivery
– Practice speech
• Several group members were not
knowledgeable on article
• Excessive use of filler words
– Work on timing and transitions
http://news.softpedia.com/images/news2/How-Dangerous-Are-Nanoparticles-2.jpg
Nanotech in
Biomedicine:
Biodegradable Polymer
Nanoparticles
for drug delivery
through mucosal Membranes
Reviewed by Group U4
PRESENTATION/ORAL REVIEW
Overall, the speakers sounded very confident,
thus enabling the audience to stay attentive to
the information presented as opposed to the flaws
in speech.
 Excellent slides with a good balance of text and
graphics. Both were large enough for the
audience in the back to see clearly
 Nice hands-on example with the
grapefruit/lemons for further explanation of the
topic.

TECHNICAL REVIEW
Perhaps this is because this research is so new,
but we would have liked to have a more in depth
description on how the mucus penetration would
work in a variety of applications.
 Also, maybe devote a little time to the future of
this technology(different diseases it could fight,
and different barriers the nanoparticle could help
break through other than the mucus)
 Overall however, good presentation that left us
with few question. The questions we did have
were answered well.

Nanotech in Biomedicine :
Biodegradable Polymer Nanoparticles
for drug delivery through mucosal Membranes
 Review of Team U3 by
Team U5 – Jaynesh
Shah, Greg Pudewell,
Edwin L. Youmsi Pete
and John Pack.
Oral and Quality of Slides
Review
 Speakers did a good job in
the oral aspect of the
presentation. They were
loud and clear confident.
Implying they had done a
good amount of research on
the topic.
 The slides clear, visible and
not overcrowded. Team U5
made good use of several
pictures, images and graphs.
Technical Review
 The presentation was
satisfying from a
technical standpoint.
 We would have
however liked them to
discuss the use of
biosensors in a little
more details with
respect to the issue of
drug delivery
Presented By Group #3
Phillip Keller
Micheal Jones
Krista Melish
James Kancewick
Critiqued by: Group U6 - Pavitra Timbalia, Michael Trevathan, Jared Walker
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The slides were well balanced with graphics and
text.
All presenters were well dressed and maintained an
appropriate tone.
The demonstration involving the fruit to
describe the receptors of drugs helped to
open the presentation and get the audiences
attention.
A few of the graphs were difficult to read and
understand, and were not explained carefully.
The evolution leading to the development
of PEG wasn’t explained until someone asked
about it during the questions section.
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This presentation dealt with the delivery of drugs
through the use of nano-particles engineered
specifically to be able to penetrate through thick
mucus that was previously not possible.
Future research needs to be done to test the
particles ability to deliver drugs
◦ The tests done for the paper only showed
that the particles were able to penetrate the
mucus - they didn’t contain any drug payload.

This technology could help to treat many diseases
such as cystic fibrosis, provided future research is
done to reduce the cost of production.
Review for U3
from G6
Jung Hwan Woo
• The table on the top left
shows the diffusivity of the
nanoparticle in mucus
compared to the diffusivity
in water. It is used to
indicate the rate at which
the nanoparticle travels in
the body through mucus.
• What are the factors that
affects these rates?
• What can be done to
improve the particle
transfer rate as suggested
on the bottom left?
Jung Hwan Woo