Transcript Слайд 1 - Science and Technology Center in Ukraine

GOLD-BEARING PROBIOTIC
DRUG “OKARIN-AU”
Workshop “From Science to Business”
11-12 October, 2006
Kyiv
Valeriya VEMBER
Telephone: +380-44-4241919
[email protected]
F.D.OVCHARENKO INSITUTE OF BIOCOLLOIDAL CHEMISTRY
NATIONAL ACADEMY OF SCIENCES OF UKRAINE
Kyiv
Proprietary information statement
• The technology material presented in this talk is
available for licensing and joint product
development.
• None of the slides contain any confidential or
proprietary information.
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The shape of gold-bearing probiotic
preparation "Okarin-Au"
2
Gastrointestinal tract disorders
deterioration of
ecological situation
increase of stressful factors
quantity and strength
using great amount
of junk food
uncontrolled usage of antibiotics
and other medicines
3
Biotherapy
Probiotic
live microorganisms;
metabolites
produced by some
microorganisms to
stimulate the growth
of other ones, etc.
Prebiotic
substances, which
selectively stimulate
the growth and
biological activity of
microorganisms in
intestines, which
positively influence
on the
microbiocenosis
structure
Probiotic food
foodstuff bearing by
probiotics
4
Main problems as to probiotic drugs’
development and application :
limited antagonistic activity of the strains-producers
to the numerous pathogenic agents;
insufficient growth speed of biomass producing
strains;
high cost of probiotic preparation as a result of
using the expensive growth substances for strainsproducers growing;
non-observance
of
the
microecological
adequacy principle while treatment of different
dysbacteriosis types have been provided
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Probiotic + Prebiotic = Synbiotic
METALS
─ important regulatory environmental compounds and
potential ingredient for Synbiotics
Bringing the appropriate metals into the organism gives good
therapeutic results in case of illnesses, which are characterised by
considerable changes in metal-microelement balance
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ADVISABILITY of PROBIOTIC DRUG
ENRICHMENT by COLLOIDAL Au and Ag
Investigation of metals influence on some
nonspecific physiological and biochemical parameters,
which have been of great importance for all the metabolic processes
that take place in bacterial cell :
Growth processes’ intensity
Cell’s wall permeability
ATP-ase activity
Level of lipid peroxidation
Energy function of bacterial plasma membrane
Antioxidant enzymes activity
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Changes of biological activity of bacterial
association “Okarin” after incubation with
GOLD (10-6 mkg/ml) and SILVER (10-7 mkg/ml)
Investigated indexes
Metals
Au
Ag
Form
Growth
intensity
PermeLevel of
+
ATP-ase
H
ability of
lipid peroxiactivity extrusion the cell
dation
wall
Activity of antioxidant
enzymes
Catalase
SOD
Colloidal





0

Ion
0
—
—
0
0

0
Colloidal
0
—
—

0
0

Ion

—
—




–
Kind of changes :
positive;
«—» – investigation is not conducted.
– negative;
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Tetracyclin
Rifampicin
Cefaleksin
Polymicsin
Cefaclor
Kanamycin
Cefazolin
Cefotaksim
Gentamycin
Ampicillin
Levomycetin
Carbenicillin
Diameter of the zone of
growth inhibition, mm
Resistance of “Okarin” and
“Okarin-Au” to the antibiotics
25
20
15
10
5
0
"Okarin-Au"
"Okarin"
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Antagonistic activity of bacterial association “Okarin”
and gold-bearing “Okarin-Au”
Microorganisms
(test object)
Size of lysis
zone after
24 hours,
mm
Quantity of test-culture (pieces) (Х ± m)
Control
after 12 hours
after 24
hours
after 72
hours
Okarin
OkarinAu
Okarin
OkarinAu
Okarin
OkarinAu
Okarin
OkarinAu
Okarin
OkarinAu
Shigella flexneri 1а
20
—
159 ± 9
—
128 ± 14
—
84 ± 7
—
12 ± 2
—
Sh. flexneri 1в
20
—
132 ± 12
—
111 ± 8
—
58 ± 7
—
14 ± 5
—
Sh. flexneri 2а
20
20
107 ± 16 107  16
92 ± 4
96  7
59 ± 6
64  8
27 ± 8
91
Sh. flexneri VI
20
20
183 ± 5
183  5
18 ± 3
73
Sh. sonnei
20
20
120 ± 7
120  7
92 ± 3
89  6
76 ± 11 51  6
25 ± 4
18  1
Salmonella enteritidis
20
20
100 ± 14 100  14
86 ± 7
78  3
51 ± 2
47  7
29 ± 8
14  3
S. thyphimurium
15
20
127 ± 16 127  16
120 ± 7
85  5 78 ± 12 68  2
25 ± 7
16  5
Staphylococcus aureus
15
20
116 ± 6
116  6
109 ± 8
73  4 59 ± 11 60  10
24 ± 10
24  5
Pseudomonas aeruginosa
15
20
—
104 ± 9 8111 79 ± 3 64  9
—
—
105 ± 2
85 ± 7
36 ± 8
31  2
—
Ps. vulgaris
*
Not find
110 2
—
122 ± 7
110 ± 2
131 ± 11 101  10 81 ± 10 47  4
─ behaviors, which show the improvement of initial preparation
“Okarin” by colloidal gold.
34 ± 9
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Innovative Aspect
and Main Advantages
 Wide
range
of
antagonistic
activity
to
pathogenic
microorganisms causing dysbacteriosis and especially
dangerous infection diseases including anthrax, rabbit-fever,
salmonellosis, brucellosis etc. (according to results of clinical trials);
 Selective bactericidal activity to pathogenic microflora and
simultaneous stimulation of normoflora. This feature is
intensified owing to the presence of colloidal gold particles;
 Low cost of the treatment course in a comparison with other probiotic
drugs which are available on the market today;
 Ability to increase the lysis of atypical pathological cells
treatment
application opportunity for oncological patients due to high resistance of
probiotic's microbial cultures to antibiotics and chematherapeutic agents;
 Non-specific immune-resistance stimulation ability of the whole organism;
 Suitable producing shape and application simplicity.
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Clinical trials’ results of
bacteriological preparation "Okarin-Au"
Dysbiosis Standard PG Standard PGT
Therapy
+ Okarin-Au
State
The number of microorganisms, lg C
45
NORMAL
State
Pathogenous species:
Staphylococcus aureus,
Enterobacter aerogenes,
Klebsiella pneumoniae,
Candida albicans
30
Normal flora of GIT:
15
0
Bifidobacterium,
Lactobacillus,
Escherichia coli
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Competitive Matrix of Probiotic
Probiotic’s
Name Okarin-Au
(IBCC-UkrInkom,
Probiotic’s
Ukraine)
Characteristic
Preparation
Composition
Antagonistic
activity (AA)
Colibacterinum
Hylak
Forte
(Biofarma,
Ukraine)
(Ratiopharm,
Germany)
E. coli (3 strains) +
E. coli (strain M-17)
Metabolites of E. coli,
Enterococcus faecalis (dried (dried alive cultures) – Streptococcus faecalis,
alive cultures, enriched by
1*1010 CFU/dose.
Lactobacillus acidophilus
colloidal Gold) – 1:1:1:3.
Ampoules.
and L. helveticus +
Capsules.
growth factors (aqueous
solution) – 2:1:1:4.
Bottles.
Shigella flexneri, Sh. sonnei, Mixed infection of
Salmonella enteritidis, S.
GIT and
Mixed infection of
thyphimurium, StaphylococSalmonellosis.
GIT and
cus aureus, Pseudomonas
aeruginosa, Ps. vulgaris,
Salmonellosis
Enterobacter aerogenes,
Prohibited to use in the
Klebsiela pneumonia,
case of Escherichiosis
Candida albicans
!!!
Linex
Bifiform
(LekPharma,
Slovakia)
(Ferrosan,
Denmark)
Lactobacillus
Bifidobacterium
acidophilus,
longum and
Bifidobacterium infantis,
Enterococcus
Enterococcus faecium faecium (dried alive
(dried alive cultures) –
cultures) – 1*107
1,2*107 CFU/dose (each CFU/dose (each of
of strains). Capsules. strains). Capsules.
Wide range
Wide range
of AA
of AA
(accordance to (accordance to
preparation
preparation
Instruction)
Instruction
Dosage regimen
(per 24 hours)
2-3 capsules
6-12 doses
120-180 drops
6 capsules
2-4 capsules
Cost of treatment
for 10 days
$ 10
$7
$ 11
$ 15
$ 12,5
―
―
Complications and
peculiarities of
application
―
1) Require preliminary
preparation;
2) Prohibited to use at
the same time with
Antibiotics
1) Require preliminary
measuring;
2) Prohibited to use at
the same time with Milk
and Dairy Produce
13
Opportunities
• We seek for potential partners interested in
new type of combined (and effective!) probiotic
preparation and partners, who can help us in
putting them on the market
• We are ready for collaboration with the
purpose of creation of different synbiotic
drugs which include wide range of colloidal
metals and have different therapeutic effects
14
Contact Information :
Prof. Zoya ULBERG
Telephone: +380 – 44 – 424 80 78
e-mail: [email protected]
F.D.OVCHARENKO INSITUTE OF BIOCOLLOIDAL CHEMISTRY
NATIONAL ACADEMY OF SCIENCES OF UKRAINE
Kyiv
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