Transcript PowerPoint Presentation - Cell Architecture: The Nucleus

The Nucleus
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Interphase Nuclear Dynamics
Time Lapse Video
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DIC Brightfield
Euchromatin
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EM
thin
section
view
Chromatin organization
early replication -> late replication
lamin B
and
late
replicated
DNA
chromosomal organization one cell cycle later
lamin B
and
early
replicated
DNA
40x condensation
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Packing
it all in
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146 bp; 6x condensation
The Histone
Code
Anchoring the genome
LAPs
(Lamin
associated
proteins)
anchor the
lamina to
the inner
envelope
Freeze etch view of the lamina
The nuclear matrix
Diversity of nuclear shapes
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Eosinophil nuclei are constricted
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Chromosomal domains
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Nuclear compartments
Promyelocytic leukemia (PML)
nuclear bodies
Video
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Total RNA production
(fluorouridine pulse)
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Nucleolar Substructure
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Mammalian cell
Yeast cell
Polymerase I transcribes
rDNA in nucleolar fibrillar centers
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500 nm
Gene Expression Factories
Euchromatin (acH3), DNA (DAPI), Spliceosomal protein SC-35
Animated
reconstruction
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Spliceosomes
contain
poly-A RNA
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3 Video Sequences
Time lapse sequences
show SC-35 protein
over 30 min periods
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Nuclear speckle
dynamics
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Collagen A1 mRNA
Nuclear
mRNA
localizations
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mRNA (red)
Gene (green)
Epstein-Barr Viral mRNAs
Nuclear traffic
Negative Stain
Freeze
Fracture
Visualizing
Nuclear
Pores
Thin Section
CryoEM Tomographic Reconstruction
Annulate lamellae store pores
400 nm
Nuclear
pores
In vitro
regulation
by Ca++
Transport of
nucleoplasmincoated gold
particles
It takes two (or three) to tango
Digitonin-permeabilized cells incubated with NLS*BSA*TRITC
-cytosol
+cytosol
+cytosol, GTP
Nup358
Nup214
Nup153
NLS*BSA
+ cytosolic factors
identifies
nucleoporins on a
Western-style blot
Nup 98
Nup 62
Nuclear Transport
Exportin
NES
Ran*GTP
Ran-GAP
Exportin
Ran*GTP
Ran-GEF
Ran*GDP
NLS
Importin
Cytoplasmic compartment
NES
Ran*GDP
NLS
Importin
Nuclear compartment
FG affinity transport
Follow the FG-repeats
Follow the FG-repeats
Follow, follow, follow, follow
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Animation
The nucleus - not just a place to
find DNA anymore
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