Transcript PPT - GCP21

Characterization of cassava
Germplasm core collection in
Rayong Field Crops Research (RYFCRC),
Department of Agriculture (DOA)
The cassava germplasm collection in Thailand
Rayong Field Crops Research (RYFCRC),
Department of Agriculture (DOA) collected the varieties
by ourselves more than 200 accessions
Table 1. The cassava germplasm collection in Rayong
Field Crops Research (RYFCRC)
collected the varieties by ourselves (279)
Sources
Local
Wild
Introduce
Virgin Island
Indonesia
Vietnam
China
Laos
CIAT
Promising
No. accessions
10
1
17
5
6
5
2
48
185
Scientific names
Manihot esculenta Grantz.
M. giaziovii
M. esculenta
M. esculenta
M. esculenta
M. esculenta
M. esculenta
M. esculenta
M. esculenta
In vitro conservation
2001,CIAT, Colombia 600 accessions.
RYFCRC collect and conserve in 2 conditions
1. In the laboratory, subculture every 3-4 months
2. In the field condition
Table 2. Cassava germplasm core collection from CIAT
variental # accessions variental # accessions variental # accessions
name
name
name
CG
12
MDOM
3
MPAN
9
CM
18
MECU
30
MPAR
39
CR
21
MFJI
2
MPER
73
MARG
8
MGUA
15
MPHI
2
MBOL
3
MHMC
1
MPTR
6
MBRA
99
MIND
7
MTAI
4
MCHN
2
MMAL
15
MUSA
4
MCOL
160
MMEX
19
MVEN
52
MCUB
18
MNGA
3
SG
2
Total 628 accessions
Propagation by tissue culture
Subculture
0-7 days
1.5-2 months
3 months
Transfer to
green house
At room temperature at 1 week
Coconut fifer
25-30 days
Field Collection
1.5 months
3-4 months
12 months
Characterization of cassava germplasm
core collection
Descriptors to be scored at 3 months after
planting
Color of Apical Leaves
Color of Apical Leaves
Record the most frequent occurrence
Color of Apical
Leaves
Descriptors to be scored at 6 months and 9
months after planting
- Petiole color
- Color of leaf vein
- Leaf color
- Color of stem cortex
- Number of leaf lobes
- Color of stem epidermis
- Length of leaf lobe
- Color of stem exterior
- Width of leaf lobe
- Distance between leaf scars
- Lobe margins
- Growth habit of stem
- Petiole length
Dark purple
purple
red
Record the most frequent occurrence
green
Redish-green
Greenish-purple
Petiole Color
Orientation of
petiole
Inclined upwards
Horizontal
Irregular
Inclined downwards
Observe from
the middle of the
plant
Descriptors to be scored at 6 months after planting Leaf Color
A leaf from the middle of the plant
Record the most frequent occurrence
purple
Purple-green
Green-purple
Green -white
Light green
Dark green
Record the most frequent occurrence
straight
Oblong-lanceolate
ovoid
Obovate-lanceolate
Shape of central leaflet
lanceolate
Elliptic-lanceolate
lanceolate
red
pink
Reddish white
white
Color of leaf vein
Descriptors to be scored at 6 months after
planting
Number of Leaf Lobes
3 lobes
5 lobes
7 lobes
7 lobes
Observe a leaf from the middle of the plant ( 5 leaves )
9 lobes
Descriptors to be scored at 6 months after
planting
Length and Width of Leaf Lobe
Measure 3 leaves from the middle of the plant ( cm )
Descriptors to be scored at 6 months after
planting
Petiole Length
Short
(≤15cm.)
Medium
(16-25cm.)
Long
(>25cm.)
Observe from the middle of the plant
Descriptors to be scored at 6 months after
planting
Flowering
Absent
Present
Descriptors to be scored at 9 months after
planting
Distance between leaf scars
Measure from the middle of stem and plant ( cm )
Divide the distance by the number of node
Short
Medium
Long
Color of stem exterior
observe on the middle of the plant ( cm )
Growth habit of stem
Zig-zag
Straight
The Descriptors to be scored at harvest
such as
- Plant height
- Height to first branching
- Levels of branching
- Branching habit
- Angle of branching
- Dry matter content
- Starch content
- Harvest index
120 CM
310 CM
Level of Branching
Plant height
Height to first branching
Record measurements from 3 plants
No branch
Branch and angle
Angle of branching- measure at first primary branching
From 3 plants
Fruit
Absent
Present
yellow
Dark brown
External color of storage root
Record the most frequent occurrence
Number of storage
roots/plant
Light brown
cream
Record the root from three
plant
Extent of root peduncle
Sessile
Pedunculate
Color of root cortex
Record the most frequent occurrence
Color of root pulp (parenchyma)
Record the most frequent occurrence
Starch content (%)
Table 3 The Morphological for characterization the
Cassava core germplasm
Character
Min
Max
Mean
1
Plant ht.(cm)
120.0
310.0
208.0
2
Stem/Plant
1.0
6.0
3.5
3
Leaf lobes/Leaf
5.0
10.0
7.0
4
Root/Plant
1.0
22.0
9.0
5
Root wt.(Kg/Plant)
0.2
8.7
2.2
6
% Starch
0.4
30.4
16.0
Table 4 Yield (t/ha) : The cassava germplasm core
collection in RYFCRC
clone
root/plant
% starch
root wt.(t./ha)
CM 3306-4
6
31
39.38
MVEN 90
10
31
25.82
CM 3306-9
10
31
25.69
MCOL 2215
10
30
30.69
MMEX 8
8
29
32.69
MCOL 2638
8
29
26.63
MCOL 1805
7
29
25.88
MCOL 1516
11
28
38.44
Rayong 7
13
32
39.38
Rayong 9
13
32
38,00
Acknowleagements
CIAT, Dr. Daniel Debouck
Dr. Hernan Ceballos
Dr. Reinhardt Hoewler
Dr. Martin Fregene
Rayong Field Crops Research center
(RYFCRC)
Department of Agriculture (DOA) , Thiland
Thank
you
for your
listening