Transcript to view or the PHOTOSYNTHESIS Presentation

Extracting Chlorophyll
Using paper chromatography
The chemical
formula for water =
H2O
Hydrogen = H
H2 = 2 atoms of Hydrogen
Oxygen = O
H2 O
Extracting Chlorophyll
Using paper chromatography
The chemical formula
for carbon dioxide =
CO2
Carbon = C
Oxygen = O
O2 = 2 atoms of Oxygen
CO2
Extracting Chlorophyll
Using paper chromatography
Scientists write equations to show
chemical reactions
The formation of water from
hydrogen and oxygen is written as:
2H2 + O2
2H2O + energy
Extracting Chlorophyll
Using paper chromatography
Scientists write equations to show
chemical reactions
2H2 + O2
Two
molecules of
water are
formed
2H2O + energy
Energy is
released
Extracting Chlorophyll
Using paper chromatography
The most important chemical
reaction in the world is…
PHOTOSYNTHESIS
The chemical reaction
for photosynthesis is
6CO2 + 6H2O + energy
C6H12O6 + 6O2
Extracting Chlorophyll
Using paper chromatography
PHOTOSYNTHESIS
Carbon Dioxide
6CO2 + 6H2O + energy
Water
Sunlight
Sugar
C6H12O6 + 6O2
Oxygen
Extracting Chlorophyll
Using paper chromatography
PHOTOSYNTHESIS
Plants possess a light
trapping molecule called
Chlorophyll
Extracting Chlorophyll
Using paper chromatography
PHOTOSYNTHESIS
Chlorophyll
Captures light energy and
uses it to make food
Gives leaves their green color
Extracting Chlorophyll
Using paper chromatography
PHOTOSYNTHESIS
Chlorophyll
Gives leaves their green color
Comes in many colors and
two forms
Chlorophyll a
Chlorophyll b
Extracting Chlorophyll
Using paper chromatography
PHOTOSYNTHESIS
Chlorophyll
Chlorophyll a
Chlorophyll b
Both forms absorb different
wavelengths of light
Extracting Chlorophyll
Using paper chromatography
PHOTOSYNTHESIS
Chlorophyll
In this paper chromatography experiment
Chlorophyll a
Will appear as a bright
yellow-green band
Chlorophyll b
Will appear as a dull
green band
Extracting Chlorophyll
Using paper chromatography
Accessory Pigments that help
transfer light to the chlorophyll
Carotene
Will appear as an
orange band
Xanthophyll
Will appear as a
yellow band
Anthocyanin
Will appear as a redbrown band
(acts as plant
sunscreen)
Extracting Chlorophyll
Using paper chromatography
Materials
Filter paper cut into strips
Pennies
Straw or stirrer
Beaker
Rubbing alcohol
Tape
Ruler
Plant leaves
Extracting Chlorophyll
Using paper chromatography
Procedure
Draw a line across the paper strip,
2 cm from one end
Place the leaf you want to test on
top of the filter paper strip,
remembering where the line is
Extracting Chlorophyll
Using paper chromatography
Procedure
Using the edge of a penny, trace
the penny across the leaf right on
top of where you drew the line
Push down hard enough to leave a
green smear on the paper on top
of the pencil mark
Extracting Chlorophyll
Using paper chromatography
Procedure
Repeat 2 more times with fresh
sections of leaf to make the smear
darker
Try to keep the smear as close to
the original pencil line as possible
Extracting Chlorophyll
Using paper chromatography
Procedure
Fill the beaker to a depth of 1 cm
with rubbing alcohol
Carefully lower the filter paper
strip, green smear end in first, into
the beaker until the bottom just
touches the alcohol
Extracting Chlorophyll
Using paper chromatography
Procedure
Make sure that the green smear
does not actually touch the
alcohol, only the tip of the paper
Lay the stick across the mouth of
the beaker like a bridge from one
edge to the other
Extracting Chlorophyll
Using paper chromatography
Procedure
Tape the filter paper strip to the
stick so that the paper is held in
place just touching the alcohol but
not touching the sides of the
beaker
Extracting Chlorophyll
Using paper chromatography
Extracting Chlorophyll
Using paper chromatography
Procedure
Wait. The alcohol should gradually
move up the paper, bringing many
of the pigments along with it
Remove the stick and paper when
the alcohol has almost reached
the stick
Extracting Chlorophyll
Using paper chromatography
Procedure
Mark how far up the paper the
alcohol traveled with a pencil
Determine how many bands of
pigment you have, what color they
are, and measure how far each
band traveled from the origin line
Extracting Chlorophyll
Using paper chromatography
Procedure
Identify the pigments you found
based on their colors
Draw a color chart of what you found
Prepare a report out for the class